Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | http://hdl.handle.net/1843/51232 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2023-03-27T12:14:06Z2023-03-27T12:14:06Z2021-09-1826342543010.17957/IJAB/15.18521814-9596http://hdl.handle.net/1843/51232CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaDiarrhea is one of the most important diseases in calves, resulting in high mortality along with the high costs of antimicrobial agents. For alternative control with probiotics, it is essential to isolate autochthonous bacteria that are effective against Escherichia coli. The aim of this study was to select lactic acid bacteria (LAB) showing probiotic potential from dairy calves raised in a semiarid tropical region. The feces of ten healthy weaned Girolando calves were sampled, and after dilution were incubated on solid de Man–Rogosa–Sharpe medium, in a microaerophilic atmosphere. A high population of LAB was observed in samples from each calf evaluated (average 2.8 × 109 colony-forming units g−. Of the 31 LAB isolates, all of which were negative for catalase production and and 61.3% were Gram-positive rods were Gram-positive rods, compatible with the Lactobacillus genus. However, only seven of these isolates showed milk coagulation capacity after successive peak and were pre-selected. In the test for screening resistance to bile salts at 1% and to pH4, growth was observed in three (42.8%) of the seven LAB. After 16S rRNA gene sequencing and proteomic analysis, a selected LAB identified as Lactobacillus plantarum enabled higher inhibition of E. coli of bovine origin than other isolates, presenting probiotic potential for the control of colibacillosis.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASInternational Journal of Agriculture and BiologyGirolando (Bovino)Escherichia coliColibaciloseDiarreia em bovinosDiarreia em bezerroSelection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid regioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.fspublishers.org/html_issue.php?i_id=41675Eduardo Robson DuarteVanessa de Araújo VelosoAniele de Cássia Rodrigues VelosoFernanda GuimarãesHugo Calixto FonsecaDanielle Soares MalveiraThiago Gomes dos Santos BrazMaximiliano Soares Pintoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/51232/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALSelection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region.pdfSelection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region.pdfapplication/pdf274078https://repositorio.ufmg.br/bitstream/1843/51232/2/Selection%20and%20molecular%20identification%20of%20lactic%20acid%20bacteria%20with%20probiotic%20potential%20from%20girolando%20calves%20raised%20in%20a%20semiarid%20region.pdf5c126d478dbb94bc57aa4bf7c60c60a2MD521843/512322023-03-27 16:49:00.022oai:repositorio.ufmg.br:1843/51232TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-03-27T19:49Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region |
| title |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region |
| spellingShingle |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region Eduardo Robson Duarte Girolando (Bovino) Escherichia coli Colibacilose Diarreia em bovinos Diarreia em bezerro |
| title_short |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region |
| title_full |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region |
| title_fullStr |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region |
| title_full_unstemmed |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region |
| title_sort |
Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region |
| author |
Eduardo Robson Duarte |
| author_facet |
Eduardo Robson Duarte Vanessa de Araújo Veloso Aniele de Cássia Rodrigues Veloso Fernanda Guimarães Hugo Calixto Fonseca Danielle Soares Malveira Thiago Gomes dos Santos Braz Maximiliano Soares Pinto |
| author_role |
author |
| author2 |
Vanessa de Araújo Veloso Aniele de Cássia Rodrigues Veloso Fernanda Guimarães Hugo Calixto Fonseca Danielle Soares Malveira Thiago Gomes dos Santos Braz Maximiliano Soares Pinto |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Eduardo Robson Duarte Vanessa de Araújo Veloso Aniele de Cássia Rodrigues Veloso Fernanda Guimarães Hugo Calixto Fonseca Danielle Soares Malveira Thiago Gomes dos Santos Braz Maximiliano Soares Pinto |
| dc.subject.other.pt_BR.fl_str_mv |
Girolando (Bovino) Escherichia coli Colibacilose Diarreia em bovinos Diarreia em bezerro |
| topic |
Girolando (Bovino) Escherichia coli Colibacilose Diarreia em bovinos Diarreia em bezerro |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2021 |
| dc.date.issued.fl_str_mv |
2021-09-18 |
| dc.date.accessioned.fl_str_mv |
2023-03-27T12:14:06Z |
| dc.date.available.fl_str_mv |
2023-03-27T12:14:06Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/1843/51232 |
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10.17957/IJAB/15.1852 |
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1814-9596 |
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10.17957/IJAB/15.1852 1814-9596 |
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http://hdl.handle.net/1843/51232 |
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eng |
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eng |
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International Journal of Agriculture and Biology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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