Screening and characterization of prophages in desulfovibrio genomes

• Autor(a) principal: Josicelli Souza Crispim
• Data de Publicação: 2018
• Outros Autores: Roberto Sousa Dias, Pedro Vidigal, Maíra Paula de Sousa, Cynthia Canêdo da Silva, Mateus Ferreira Santana, Sérgio Oliveira de Paula
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: http://hdl.handle.net/1843/43349
• topic: Bactérias Redutoras de Sulfato (SRB); Desulfovibrio; Bactérias

Bacteria of the genus Desulfovibrio belong to the group of Sulphate Reducing Bacteria (SRB). SRB generate signifcant liabilities in the petroleum industry, mainly due to their ability to microbiologically induce corrosion, bioflm formation and H2S production. Bacteriophages are an alternative control method for SRB, whose information for this group of bacteria however, is scarce. The present study developed a workfow for the identifcation of complete prophages in Desulfovibrio. Poly-lysogenesis was shown to be common in Desulfovibrio. In the 47 genomes analyzed 53 complete prophages were identifed. These were classifed within the order Caudovirales, with 69.82% belonging to the Myoviridade family. More than half the prophages identifed have genes coding for lysozyme or holin. Four of the analyzed bacterial genomes present prophages with identity above 50% in the same strain, whose comparative analysis demonstrated the existence of colinearity between the sequences. Of the 17 closed bacterial genomes analyzed, 6 have the CRISPR-Cas system classifed as inactive. The identifcation of bacterial poly-lysogeny, the proximity between the complete prophages and the possible inactivity of the CRISPR-Cas in closed bacterial genomes analyzed allowed the choice of polylysogenic strains with prophages belonging to the Myoviridae family for the isolation of prophages and testing of related strains for subsequent studies.