FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters

Detalhes bibliográficos
Autor(a) principal: Gregório Guilherme Almeida
Data de Publicação: 2021
Outros Autores: Fernanda Morcatti Coura, Jonata de Melo Barbieri, Ana Carolina Junqueira Moura, Fabiola de Oliveira Paes Leme, Adriane Pimenta da Costa-Val
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1155/2021/3192960
http://hdl.handle.net/1843/67437
https://orcid.org/0000-0003-1108-1160
https://orcid.org/0000-0003-2243-3125
https://orcid.org/0000-0003-1044-3473
https://orcid.org/0000-0001-7555-1956
https://orcid.org/0000-0002-4013-0147
https://orcid.org/0000-0001-8995-9805
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling 2024-04-18T20:40:46Z2024-04-18T20:40:46Z20212021https://doi.org/10.1155/2021/31929602314-6141http://hdl.handle.net/1843/67437https://orcid.org/0000-0003-1108-1160https://orcid.org/0000-0003-2243-3125https://orcid.org/0000-0003-1044-3473https://orcid.org/0000-0001-7555-1956https://orcid.org/0000-0002-4013-0147https://orcid.org/0000-0001-8995-9805CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoDogs are the main reservoir of Leishmania infantum in endemic regions. Canine leishmaniasis, caused by L. infantum, can progress to a chronic disease resulting in death. Vaccines have been developed with a certain degree of success. The pathogenesis of this disease is not completely understood, especially in previously vaccinated dogs. We herein described clinical data, parasite load, serum levels of cytokines, and the reservoir potential in vdogs vaccinated with the fucose-mannose ligand (FML)/QuilA saponin vaccine (Leishmune™) naturally infected (Vi) and compared to vaccinated not infected dogs (Vn). Thirty-four dogs from private owners were divided into two groups: vaccinated/infected and vaccinated/uninfected. Clinical evaluation, hematological and biochemical parameters, and serum levels of cytokines were measured by conventional methods. The parasite burden in the bone marrow was measured by quantitative real-time PCR, and the transmissibility of parasites to sand flies was assessed by xenodiagnosis. Clinical, biochemical, and hematological parameters of vaccinated infected dogs were mostly normal. Vi dogs developed mild disease with low clinical scores. Serum levels of IL-10 were higher in Vi dogs, and a strong correlation was observed in IL-4 levels and the A/G ratio in Vi dogs. These results suggest a role of TH2 response in Vi dogs, although more data is needed to better understand the disease in vaccinated dogs.engUniversidade Federal de Minas GeraisUFMGBrasilVET - DEPARTAMENTO DE CLÍNICA E CIRURGIABioMed Research InternationalLeishmania infantumVacinasCitocinasCãesLeishmania infantumVaccinesCytokinesVaccinated dogsFML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parametersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.hindawi.com/journals/bmri/2021/3192960/Gregório Guilherme AlmeidaFernanda Morcatti CouraJonata de Melo BarbieriAna Carolina Junqueira MouraFabiola de Oliveira Paes LemeAdriane Pimenta da Costa-Valinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/67437/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALFML_QuilA-vaccinated dogs naturally infected with Leishmania infantum_ serum cytokines, clinicopathological profile, and parasitological parameters.pdfFML_QuilA-vaccinated dogs naturally infected with Leishmania infantum_ serum cytokines, clinicopathological profile, and parasitological parameters.pdfapplication/pdf247341https://repositorio.ufmg.br/bitstream/1843/67437/2/FML_QuilA-vaccinated%20dogs%20naturally%20infected%20with%20Leishmania%20infantum_%20serum%20cytokines%2c%20clinicopathological%20profile%2c%20and%20parasitological%20parameters.pdfd236be620411d14ef8f994ea95b3e1b3MD521843/674372024-04-18 17:40:47.288oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2024-04-18T20:40:47Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
title FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
spellingShingle FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
Gregório Guilherme Almeida
Leishmania infantum
Vaccines
Cytokines
Vaccinated dogs
Leishmania infantum
Vacinas
Citocinas
Cães
title_short FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
title_full FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
title_fullStr FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
title_full_unstemmed FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
title_sort FML/QuilA-vaccinated dogs naturally infected with Leishmania infantum: serum cytokines, clinicopathological profile, and parasitological parameters
author Gregório Guilherme Almeida
author_facet Gregório Guilherme Almeida
Fernanda Morcatti Coura
Jonata de Melo Barbieri
Ana Carolina Junqueira Moura
Fabiola de Oliveira Paes Leme
Adriane Pimenta da Costa-Val
author_role author
author2 Fernanda Morcatti Coura
Jonata de Melo Barbieri
Ana Carolina Junqueira Moura
Fabiola de Oliveira Paes Leme
Adriane Pimenta da Costa-Val
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gregório Guilherme Almeida
Fernanda Morcatti Coura
Jonata de Melo Barbieri
Ana Carolina Junqueira Moura
Fabiola de Oliveira Paes Leme
Adriane Pimenta da Costa-Val
dc.subject.por.fl_str_mv Leishmania infantum
Vaccines
Cytokines
Vaccinated dogs
topic Leishmania infantum
Vaccines
Cytokines
Vaccinated dogs
Leishmania infantum
Vacinas
Citocinas
Cães
dc.subject.other.pt_BR.fl_str_mv Leishmania infantum
Vacinas
Citocinas
Cães
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2024-04-18T20:40:46Z
dc.date.available.fl_str_mv 2024-04-18T20:40:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/67437
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1155/2021/3192960
dc.identifier.issn.pt_BR.fl_str_mv 2314-6141
dc.identifier.orcid.pt_BR.fl_str_mv https://orcid.org/0000-0003-1108-1160
https://orcid.org/0000-0003-2243-3125
https://orcid.org/0000-0003-1044-3473
https://orcid.org/0000-0001-7555-1956
https://orcid.org/0000-0002-4013-0147
https://orcid.org/0000-0001-8995-9805
url https://doi.org/10.1155/2021/3192960
http://hdl.handle.net/1843/67437
https://orcid.org/0000-0003-1108-1160
https://orcid.org/0000-0003-2243-3125
https://orcid.org/0000-0003-1044-3473
https://orcid.org/0000-0001-7555-1956
https://orcid.org/0000-0002-4013-0147
https://orcid.org/0000-0001-8995-9805
identifier_str_mv 2314-6141
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv BioMed Research International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/67437/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/67437/2/FML_QuilA-vaccinated%20dogs%20naturally%20infected%20with%20Leishmania%20infantum_%20serum%20cytokines%2c%20clinicopathological%20profile%2c%20and%20parasitological%20parameters.pdf
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