Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1371/journal.pone.0164946 http://hdl.handle.net/1843/40109 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2022-03-15T17:53:25Z2022-03-15T17:53:25Z20161110115https://doi.org/10.1371/journal.pone.01649461932-6203http://hdl.handle.net/1843/40109CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)The zoonotic potential of giardiasis, as proposed by WHO since the late 70's, has been largely confirmed in this century. The genetic assemblages A and B of Giardia duodenalis are frequently isolated from human and canine hosts. Most of the assemblage A strains are not infective to adult mice, which can limit the range of studies regarding to biology of G. duodenalis, including virulence factors and the interaction with host immune system. This study aimed to determine the infectivity in mice of an assemblage A Giardia duodenalis strain (BHFC1) isolated from a dog and to classify the strain in sub-assemblages (AI, AII, AIII) through the phylogenetic analysis of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. In addition, the proteomic profile of soluble and insoluble protein fractions of trophozoites was analyzed by 2D-electrophoresis. Accordingly, trophozoites of BHFC1 were highly infective to Swiss mice. The phylogenetic analysis of tpi and gdh revealed that BHFC1 clustered to sub-assemblage AI. The proteomic map of soluble and insoluble protein fractions led to the identification of 187 proteins of G. duodenalis, 27 of them corresponding to hypothetical proteins. Considering both soluble and soluble fractions, the vast majority of the identified proteins (n = 82) were classified as metabolic proteins, mainly associated with carbon and lipid metabolism, including 53 proteins with catalytic activity. Some of the identified proteins correspond to antigens while others can be correlated with virulence. Besides a significant complementation to the proteomic data of G. duodenalis, these data provide an important source of information for future studies on various aspects of the biology of this parasite, such as virulence factors and host and pathogen interactions.engUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASICB - DEPARTAMENTO DE MICROBIOLOGIAICB - DEPARTAMENTO DE PARASITOLOGIAPlos OneGiardíaseGiardia lambliaProteômicaTrofozoítosFilogeniaGenotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164946Camila Henriques CoelhoAdriana Oliveira CostaAna Carolina Carvalho SilvaMaíra Mazzoni PucciÂngela Vieira SerufoHaendel Gonçalves Nogueira Oliveira BusattiMaurício DuriganJonas Enrique Aguilar PeralesDonat Alexander de ChapeaurougeDaniel Almeida da Silva e SilvaMaria Aparecida GomesJuliano Simões ToledoSteven M. SingerRosiane Aparecida da Silva PereiraAna Paula Salles Moura Fernandesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/40109/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALGenotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice.pdfGenotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice.pdfapplication/pdf1474625https://repositorio.ufmg.br/bitstream/1843/40109/2/Genotyping%20and%20descriptive%20proteomics%20of%20a%20potential%20zoonotic%20canine%20strain%20of%20Giardia%20duodenalis%2c%20infective%20to%20mice.pdf8251afc9e1ce14a5cdb9802601367aafMD521843/401092022-03-15 14:53:25.42oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-03-15T17:53:25Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice |
title |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice |
spellingShingle |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice Camila Henriques Coelho Giardíase Giardia lamblia Proteômica Trofozoítos Filogenia |
title_short |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice |
title_full |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice |
title_fullStr |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice |
title_full_unstemmed |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice |
title_sort |
Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice |
author |
Camila Henriques Coelho |
author_facet |
Camila Henriques Coelho Adriana Oliveira Costa Ana Carolina Carvalho Silva Maíra Mazzoni Pucci Ângela Vieira Serufo Haendel Gonçalves Nogueira Oliveira Busatti Maurício Durigan Jonas Enrique Aguilar Perales Donat Alexander de Chapeaurouge Daniel Almeida da Silva e Silva Maria Aparecida Gomes Juliano Simões Toledo Steven M. Singer Rosiane Aparecida da Silva Pereira Ana Paula Salles Moura Fernandes |
author_role |
author |
author2 |
Adriana Oliveira Costa Ana Carolina Carvalho Silva Maíra Mazzoni Pucci Ângela Vieira Serufo Haendel Gonçalves Nogueira Oliveira Busatti Maurício Durigan Jonas Enrique Aguilar Perales Donat Alexander de Chapeaurouge Daniel Almeida da Silva e Silva Maria Aparecida Gomes Juliano Simões Toledo Steven M. Singer Rosiane Aparecida da Silva Pereira Ana Paula Salles Moura Fernandes |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Camila Henriques Coelho Adriana Oliveira Costa Ana Carolina Carvalho Silva Maíra Mazzoni Pucci Ângela Vieira Serufo Haendel Gonçalves Nogueira Oliveira Busatti Maurício Durigan Jonas Enrique Aguilar Perales Donat Alexander de Chapeaurouge Daniel Almeida da Silva e Silva Maria Aparecida Gomes Juliano Simões Toledo Steven M. Singer Rosiane Aparecida da Silva Pereira Ana Paula Salles Moura Fernandes |
dc.subject.other.pt_BR.fl_str_mv |
Giardíase Giardia lamblia Proteômica Trofozoítos Filogenia |
topic |
Giardíase Giardia lamblia Proteômica Trofozoítos Filogenia |
description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2022-03-15T17:53:25Z |
dc.date.available.fl_str_mv |
2022-03-15T17:53:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/40109 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1371/journal.pone.0164946 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
url |
https://doi.org/10.1371/journal.pone.0164946 http://hdl.handle.net/1843/40109 |
identifier_str_mv |
1932-6203 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS ICB - DEPARTAMENTO DE MICROBIOLOGIA ICB - DEPARTAMENTO DE PARASITOLOGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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Repositório Institucional da UFMG |
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