Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune

Detalhes bibliográficos
Autor(a) principal: Viviane de Paula Lima
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMG
Texto Completo: http://hdl.handle.net/1843/ZMRO-89LN6H
Resumo: Furcation perforation is a mechanical or pathologic communication between the root canal system and the external tooth surface. Nowadays, MTA is the best material to treat perforation because it is biocompatible and has a good sealing ability. The aim of this study was to evaluate the expression of cytokines in the presence of MTA used to treat an induced furcation perforation in mouse. BALB/c mouse were used (n=5). The first upper molar had its furcation perforated and treated with MTA in the left side (experimental group) while in the right side, the furcation was not treated (control group). The animals were killed in 7, 14 and 21 days after the intervention. The teeth and the around tissue were extracted and mashed. Then, the RNA was extracted. The expressions of the cytokines IFN-, TNF-, IL-10, IL-4, TGF- e RANKL were investigated by real time PCR. Comparing the experimental group with the control group, only IL-4 exhibited statistical difference (p<0.05). In 07 days, there was a lower expression (p<0.05) of TNF- and IL-4 comparing to 14 days. The expression of RANKL, IFN- and TNF- demonstrated to be statistically higher (p<0.05) in 14 days comparing to 21 days. IL-10 expressed increased (p<0.05) in 21 days. The expression of TGF- did not exhibit results with statistic relevance. So, it seems that MTA favored the expression of pro-inflammatory cytokines in an intermediate phase of imuno-inflammatory response (14 days) and a reduction of these cytokines in the later phase of the response, probably due to an IL-10 imunoregulation.
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spelling Perfurações experimentais de furca tratadas com MTA: Análise da resposta imunecitocinas inflamatóriasperfuraçãoMTACimentos dentáriosCanal radicular TratamentoBiocompatibilidadeRelação dose-resposta imunológicaMateriais restauradores do canal radicularCitocinasFurcation perforation is a mechanical or pathologic communication between the root canal system and the external tooth surface. Nowadays, MTA is the best material to treat perforation because it is biocompatible and has a good sealing ability. The aim of this study was to evaluate the expression of cytokines in the presence of MTA used to treat an induced furcation perforation in mouse. BALB/c mouse were used (n=5). The first upper molar had its furcation perforated and treated with MTA in the left side (experimental group) while in the right side, the furcation was not treated (control group). The animals were killed in 7, 14 and 21 days after the intervention. The teeth and the around tissue were extracted and mashed. Then, the RNA was extracted. The expressions of the cytokines IFN-, TNF-, IL-10, IL-4, TGF- e RANKL were investigated by real time PCR. Comparing the experimental group with the control group, only IL-4 exhibited statistical difference (p<0.05). In 07 days, there was a lower expression (p<0.05) of TNF- and IL-4 comparing to 14 days. The expression of RANKL, IFN- and TNF- demonstrated to be statistically higher (p<0.05) in 14 days comparing to 21 days. IL-10 expressed increased (p<0.05) in 21 days. The expression of TGF- did not exhibit results with statistic relevance. So, it seems that MTA favored the expression of pro-inflammatory cytokines in an intermediate phase of imuno-inflammatory response (14 days) and a reduction of these cytokines in the later phase of the response, probably due to an IL-10 imunoregulation.A perfuração de furca é uma comunicação mecânica ou patológica entre o sistema de canais radiculares e a superfície externa do dente. Atualmente, o MTA é o material mais indicado no tratamento dessas perfurações, por ser biocompatível e apresentar bom selamento. O objetivo desse estudo foi avaliar a expressão de citocinas inflamatórias na presença de MTA em perfurações de furca induzidas em camundongos. O modelo de estudo, foram utilizados camundongos BALB/c (n=5). O primeiro molar superior teve a furca perfurada e tratada com MTA no lado esquerdo (grupo experimental) e, no lado direito, a furca foi perfurada e não tratada (grupo controle). Os animais foram sacrificados com 07, 14 e 21 dias após a intervenção. Os tecidos perirradiculares adjacentes à lesão foram extraídos e macerados, fazendo-se, a seguir, a extração do RNA. Dosaram-se as expressões das citocinas IFN-, TNF-, IL-10, IL-4, TGF- e RANKL por real time PCR. Ao se comparar o grupo experimental com o controle, somente a IL-4 apresentou diferença estatística (p<0,05). Com 07 dias, observou-se menor expressão (p<0,05) do TNF- e IL-4 comparados com 14 dias. A expressão do RANKL, IFN- e TNF- demonstrou-se maior estatisticamente (p<0,05) com 14 dias ao compará-la com 21 dias. A IL-10 apresentou aumento estatístico (p<0,05) em 21 dias. A expressão do TGF- não apresentou resultados com relevância estatística. Assim, parece que o MTA favoreceu a expressão de citocinas pró-inflamatórias em uma fase intermediária da resposta imuno-inflamatória (14 dias) e redução da expressão dessas citocinas na fase tardia da resposta (21 dias), provavelmente devido a imunoregulação pela IL-10.Universidade Federal de Minas GeraisUFMGAntonio Paulino Ribeiro SobrinhoTaia Maria Berto RezendeMaria Guiomar de Azevedo BahiaMarcelo Jose Barbosa SilvaViviane de Paula Lima2019-08-14T14:11:11Z2019-08-14T14:11:11Z2010-07-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/1843/ZMRO-89LN6Hinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2019-11-14T17:38:49Zoai:repositorio.ufmg.br:1843/ZMRO-89LN6HRepositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2019-11-14T17:38:49Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
title Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
spellingShingle Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
Viviane de Paula Lima
citocinas inflamatórias
perfuração
MTA
Cimentos dentários
Canal radicular Tratamento
Biocompatibilidade
Relação dose-resposta imunológica
Materiais restauradores do canal radicular
Citocinas
title_short Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
title_full Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
title_fullStr Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
title_full_unstemmed Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
title_sort Perfurações experimentais de furca tratadas com MTA: Análise da resposta imune
author Viviane de Paula Lima
author_facet Viviane de Paula Lima
author_role author
dc.contributor.none.fl_str_mv Antonio Paulino Ribeiro Sobrinho
Taia Maria Berto Rezende
Maria Guiomar de Azevedo Bahia
Marcelo Jose Barbosa Silva
dc.contributor.author.fl_str_mv Viviane de Paula Lima
dc.subject.por.fl_str_mv citocinas inflamatórias
perfuração
MTA
Cimentos dentários
Canal radicular Tratamento
Biocompatibilidade
Relação dose-resposta imunológica
Materiais restauradores do canal radicular
Citocinas
topic citocinas inflamatórias
perfuração
MTA
Cimentos dentários
Canal radicular Tratamento
Biocompatibilidade
Relação dose-resposta imunológica
Materiais restauradores do canal radicular
Citocinas
description Furcation perforation is a mechanical or pathologic communication between the root canal system and the external tooth surface. Nowadays, MTA is the best material to treat perforation because it is biocompatible and has a good sealing ability. The aim of this study was to evaluate the expression of cytokines in the presence of MTA used to treat an induced furcation perforation in mouse. BALB/c mouse were used (n=5). The first upper molar had its furcation perforated and treated with MTA in the left side (experimental group) while in the right side, the furcation was not treated (control group). The animals were killed in 7, 14 and 21 days after the intervention. The teeth and the around tissue were extracted and mashed. Then, the RNA was extracted. The expressions of the cytokines IFN-, TNF-, IL-10, IL-4, TGF- e RANKL were investigated by real time PCR. Comparing the experimental group with the control group, only IL-4 exhibited statistical difference (p<0.05). In 07 days, there was a lower expression (p<0.05) of TNF- and IL-4 comparing to 14 days. The expression of RANKL, IFN- and TNF- demonstrated to be statistically higher (p<0.05) in 14 days comparing to 21 days. IL-10 expressed increased (p<0.05) in 21 days. The expression of TGF- did not exhibit results with statistic relevance. So, it seems that MTA favored the expression of pro-inflammatory cytokines in an intermediate phase of imuno-inflammatory response (14 days) and a reduction of these cytokines in the later phase of the response, probably due to an IL-10 imunoregulation.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-26
2019-08-14T14:11:11Z
2019-08-14T14:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/ZMRO-89LN6H
url http://hdl.handle.net/1843/ZMRO-89LN6H
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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