Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1126/sciadv.abn7424 http://hdl.handle.net/1843/60527 https://orcid.org/0000-0003-3357-9816 https://orcid.org/0000-0002-7852-0300 https://orcid.org/0000-0003-1578-6678 https://orcid.org/0000-0002-9663-4731 https://orcid.org/0000-0002-7531-0607 https://orcid.org/0000-0002-7922-0422 https://orcid.org/0000-0002-1416-8694 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2023-11-06T20:47:11Z2023-11-06T20:47:11Z2022-05-13819eabn74243https://doi.org/10.1126/sciadv.abn74242571-9262http://hdl.handle.net/1843/60527https://orcid.org/0000-0003-3357-9816https://orcid.org/0000-0002-7852-0300https://orcid.org/0000-0003-1578-6678https://orcid.org/0000-0002-9663-4731https://orcid.org/0000-0002-7531-0607https://orcid.org/0000-0002-7922-0422https://orcid.org/0000-0002-1416-8694CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorSerum-based ELISA (enzyme-linked immunosorbent assay) has been widely used to detect anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. However, to date, no study has investigated patient urine as a biological sample to detect SARS-CoV-2 virus-specific antibodies. An in-house urine-based ELISA was developed using recombinant SARS-CoV-2 nucleocapsid protein. The presence of SARS-CoV-2 antibodies in urine was established, with 94% sensitivity and 100% specificity for the detection of anti–SARS-CoV-2 antibodies with the urine-based ELISA and 88% sensitivity and 100% specificity with a paired serum-based ELISA. The urine-based ELISA that detects anti–SARS-CoV-2 antibodies is a noninvasive method with potential application as a facile COVID-19 immunodiagnostic platform, which can be used to report the extent of exposure at the population level and/or to assess the risk of infection at the individual level.engUniversidade Federal de Minas GeraisUFMGBrasilMED - DEPARTAMENTO DE CLÍNICA MÉDICAMED - DEPARTAMENTO DE MEDICINA PREVENTIVA SOCIALMEDICINA - FACULDADE DE MEDICINAScience AdvancesImunologiaBiotecnologiaCOVID-19UrinaDiagnósticoDiagnósticoCOVID-19UrinaDetecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.science.org/doi/10.1126/sciadv.abn7424Fernanda LudolfFernanda Fonseca RamosEduardo Antonio Ferraz CoelhoFlávia F. BagnoJoão A. Oliveira-da-SilvaThiago A. R. ReisMyron ChristodoulidesPaula F. VassalloCecilia G. RavettiVandack NobreFlavio G. da Fonsecaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/60527/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALDetecting anti–SARS-CoV-2 antibodies in urine samples_ A noninvasive and sensitive way to assay COVID-19 immune conversion.pdfDetecting anti–SARS-CoV-2 antibodies in urine samples_ A noninvasive and sensitive way to assay COVID-19 immune conversion.pdfapplication/pdf714509https://repositorio.ufmg.br/bitstream/1843/60527/2/Detecting%20anti%e2%80%93SARS-CoV-2%20antibodies%20in%20urine%20samples_%20A%20noninvasive%20and%20sensitive%20way%20to%20assay%20COVID-19%20immune%20conversion.pdf032db2e5d1a47e504177c1bbe2dd8b8dMD521843/605272023-11-06 17:47:11.408oai:repositorio.ufmg.br:1843/60527Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-11-06T20:47:11Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| title |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| spellingShingle |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion Fernanda Ludolf Diagnóstico COVID-19 Urina Imunologia Biotecnologia COVID-19 Urina Diagnóstico |
| title_short |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| title_full |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| title_fullStr |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| title_full_unstemmed |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| title_sort |
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| author |
Fernanda Ludolf |
| author_facet |
Fernanda Ludolf Fernanda Fonseca Ramos Eduardo Antonio Ferraz Coelho Flávia F. Bagno João A. Oliveira-da-Silva Thiago A. R. Reis Myron Christodoulides Paula F. Vassallo Cecilia G. Ravetti Vandack Nobre Flavio G. da Fonseca |
| author_role |
author |
| author2 |
Fernanda Fonseca Ramos Eduardo Antonio Ferraz Coelho Flávia F. Bagno João A. Oliveira-da-Silva Thiago A. R. Reis Myron Christodoulides Paula F. Vassallo Cecilia G. Ravetti Vandack Nobre Flavio G. da Fonseca |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fernanda Ludolf Fernanda Fonseca Ramos Eduardo Antonio Ferraz Coelho Flávia F. Bagno João A. Oliveira-da-Silva Thiago A. R. Reis Myron Christodoulides Paula F. Vassallo Cecilia G. Ravetti Vandack Nobre Flavio G. da Fonseca |
| dc.subject.por.fl_str_mv |
Diagnóstico COVID-19 Urina |
| topic |
Diagnóstico COVID-19 Urina Imunologia Biotecnologia COVID-19 Urina Diagnóstico |
| dc.subject.other.pt_BR.fl_str_mv |
Imunologia Biotecnologia COVID-19 Urina Diagnóstico |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2022 |
| dc.date.issued.fl_str_mv |
2022-05-13 |
| dc.date.accessioned.fl_str_mv |
2023-11-06T20:47:11Z |
| dc.date.available.fl_str_mv |
2023-11-06T20:47:11Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/60527 |
| dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1126/sciadv.abn7424 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2571-9262 |
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https://orcid.org/0000-0003-3357-9816 https://orcid.org/0000-0002-7852-0300 https://orcid.org/0000-0003-1578-6678 https://orcid.org/0000-0002-9663-4731 https://orcid.org/0000-0002-7531-0607 https://orcid.org/0000-0002-7922-0422 https://orcid.org/0000-0002-1416-8694 |
| url |
https://doi.org/10.1126/sciadv.abn7424 http://hdl.handle.net/1843/60527 https://orcid.org/0000-0003-3357-9816 https://orcid.org/0000-0002-7852-0300 https://orcid.org/0000-0003-1578-6678 https://orcid.org/0000-0002-9663-4731 https://orcid.org/0000-0002-7531-0607 https://orcid.org/0000-0002-7922-0422 https://orcid.org/0000-0002-1416-8694 |
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2571-9262 |
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eng |
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eng |
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Science Advances |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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MED - DEPARTAMENTO DE CLÍNICA MÉDICA MED - DEPARTAMENTO DE MEDICINA PREVENTIVA SOCIAL MEDICINA - FACULDADE DE MEDICINA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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