Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/54960 |
Resumo: | Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4C or CD8C cell were assessed by flow cytometry. The CD4C cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8C cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN gammaC cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies. |
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2023-06-15T19:22:47Z2023-06-15T19:22:47Z2017-02-211351040105010.1080/21645515.2016.126454721645515http://hdl.handle.net/1843/54960Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4C or CD8C cell were assessed by flow cytometry. The CD4C cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8C cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN gammaC cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIAMED - DEPARTAMENTO DE CLÍNICA MÉDICAHuman Vaccines & ImmunotherapeuticsInterferon betaInterleucina-10TuberculoseVacciniaInterferon betaInterleucina-10TuberculosisVacineMycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.tandfonline.com/doi/full/10.1080/21645515.2016.1264547Pryscilla Fanini WowkFlávia Dias Coelho SilvaSolange Alves VinhasOlindo Assis Martins FilhoMoisés PalaciCélio Lopes SilvaVânia Luiza Deperon BonatoLuís Henrique FrancoDenise Morais da FonsecaMarina Oliveira PaulaÉlcio Dos Santos Oliveira ViannaAna Paula Barbosa WedlingValéria Maria AugustoSilvana Maria Elói SantosAndréa Teixeira Carvalhoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/54960/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALMycobacterial Hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients pdfa.pdfMycobacterial Hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients pdfa.pdfapplication/pdf3365727https://repositorio.ufmg.br/bitstream/1843/54960/2/Mycobacterial%20Hsp65%20antigen%20upregulates%20the%20cellular%20immune%20response%20of%20healthy%20individuals%20compared%20with%20tuberculosis%20patients%20pdfa.pdfa9bacb60c96cef0701ced237ee1dcdffMD521843/549602023-06-15 16:55:14.871oai:repositorio.ufmg.br:1843/54960TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-06-15T19:55:14Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients |
title |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients |
spellingShingle |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients Pryscilla Fanini Wowk Interferon beta Interleucina-10 Tuberculosis Vacine Interferon beta Interleucina-10 Tuberculose Vaccinia |
title_short |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients |
title_full |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients |
title_fullStr |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients |
title_full_unstemmed |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients |
title_sort |
Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients |
author |
Pryscilla Fanini Wowk |
author_facet |
Pryscilla Fanini Wowk Flávia Dias Coelho Silva Solange Alves Vinhas Olindo Assis Martins Filho Moisés Palaci Célio Lopes Silva Vânia Luiza Deperon Bonato Luís Henrique Franco Denise Morais da Fonseca Marina Oliveira Paula Élcio Dos Santos Oliveira Vianna Ana Paula Barbosa Wedling Valéria Maria Augusto Silvana Maria Elói Santos Andréa Teixeira Carvalho |
author_role |
author |
author2 |
Flávia Dias Coelho Silva Solange Alves Vinhas Olindo Assis Martins Filho Moisés Palaci Célio Lopes Silva Vânia Luiza Deperon Bonato Luís Henrique Franco Denise Morais da Fonseca Marina Oliveira Paula Élcio Dos Santos Oliveira Vianna Ana Paula Barbosa Wedling Valéria Maria Augusto Silvana Maria Elói Santos Andréa Teixeira Carvalho |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pryscilla Fanini Wowk Flávia Dias Coelho Silva Solange Alves Vinhas Olindo Assis Martins Filho Moisés Palaci Célio Lopes Silva Vânia Luiza Deperon Bonato Luís Henrique Franco Denise Morais da Fonseca Marina Oliveira Paula Élcio Dos Santos Oliveira Vianna Ana Paula Barbosa Wedling Valéria Maria Augusto Silvana Maria Elói Santos Andréa Teixeira Carvalho |
dc.subject.por.fl_str_mv |
Interferon beta Interleucina-10 Tuberculosis Vacine |
topic |
Interferon beta Interleucina-10 Tuberculosis Vacine Interferon beta Interleucina-10 Tuberculose Vaccinia |
dc.subject.other.pt_BR.fl_str_mv |
Interferon beta Interleucina-10 Tuberculose Vaccinia |
description |
Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4C or CD8C cell were assessed by flow cytometry. The CD4C cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8C cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN gammaC cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-02-21 |
dc.date.accessioned.fl_str_mv |
2023-06-15T19:22:47Z |
dc.date.available.fl_str_mv |
2023-06-15T19:22:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/54960 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1080/21645515.2016.1264547 |
dc.identifier.issn.pt_BR.fl_str_mv |
21645515 |
identifier_str_mv |
10.1080/21645515.2016.1264547 21645515 |
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http://hdl.handle.net/1843/54960 |
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eng |
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eng |
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Human Vaccines & Immunotherapeutics |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
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Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA MED - DEPARTAMENTO DE CLÍNICA MÉDICA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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