Association of haemostatic and inflammatory biomarkers with nephropathy in type 1 diabetes mellitus

• Autor(a) principal: Caroline Pereira Domingueti
• Data de Publicação: 2016
• Outros Autores: Rodrigo Bastos Fóscolo, Janice Sepúlveda Reis, Fernanda Magalhães Freire Campos, Luci Maria SantAna Dusse, Maria das Graças Carvalho, Karina Braga Gomes Borges, Ana Paula Salles Moura Fernandes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1155/2016/2315260; http://hdl.handle.net/1843/57740
• topic: Diabetes mellitus; Diabetic nephropathies; Renal insufficiency, chronic; Biomarkers; Diabetes mellitus; Nefropatias diabéticas; Insuficiência renal crônica; Biomarcadores

This study aimed at investigating the association between haemostatic biomarkers, proinflammatory, and anti-inflammatory cytokines with chronic kidney disease in type 1 diabetic patients. Patients were divided into two groups: with nephropathy (albuminuria ≥ 30 mg/g and/or GFR < 60 mL/min/1.73 m2 ), = 65; and without nephropathy (albuminuria < 30 mg/g and GFR ≥ 60 mL/min/1.73 m2 ), = 60. INF-, IL-6, IL-10, and TNF- plasma levels were determined by flow cytometry. VWF, ADAMTS13 antigen, and D-Dimer plasma levels were determined by enzyme-linked immunosorbent assay and ADAMTS13 activity was assessed by fluorescence resonance energy transfer assay. Elevated levels of INF-, VWF, ADAMTS13 antigen, D-Dimer, and reduced ADAMTS13 activity/antigen ratio were observed in patients with nephropathy as compared to those without nephropathy ( = 0.001, < 0.001, < 0.001, < 0.001, and < 0.001, resp.). Cytokines and haemostatic biomarkers remained associated with nephropathy after adjustments (use of statin, acetylsalicylic acid, angiotensin converting enzyme inhibitor, and angiotensin antagonist). INF-, TNF-, and IL-10 significantly correlated with haemostatic biomarkers. Inflammatory and hypercoagulability status are associated with nephropathy in type 1 diabetes mellitus and an interrelationship between them may play an important role in pathogenesis of diabetic nephropathy.