Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/41454 |
Resumo: | Traumatic spinal cord injury results in severe neurological deficits, mostly irreversible. The cell therapy represents a strategy for treatment particularly with the use of stem cells with satisfactory results in several experimental models. The aim of the study was to compare the treatment of spinal cord injury (SCI) with and without mesenchymal stem cells (MSC), to investigate whether MSCs migrate and/or remain at the site of injury, and to analyze the effects of MSCs on inflammation, astrocytic reactivity and activation of endogenous stem cells. Three hours after SCI, animals received bone marrow-derived MSCs (1×107 in 1mL PBS, IV). Animals were euthanized 24 hours, 7 and 21 days post-injury. The MSC were not present in the site of the lesion and the immunofluorescent evaluation showed significant attenuation of inflammatory response with reduction in macrophages labeled with anti-CD68 antibody (ED1), decreased immunoreactivity of astrocytes (GFAP+) and greater activation of endogenous stem cells (nestin+) in the treated groups. Therefore, cell transplantation have a positive effect on recovery from traumatic spinal cord injury possibly due to the potential of MSCs to attenuate the immune response. |
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2022-05-06T22:59:25Z2022-05-06T22:59:25Z2016373135513681676-546Xhttp://hdl.handle.net/1843/41454Traumatic spinal cord injury results in severe neurological deficits, mostly irreversible. The cell therapy represents a strategy for treatment particularly with the use of stem cells with satisfactory results in several experimental models. The aim of the study was to compare the treatment of spinal cord injury (SCI) with and without mesenchymal stem cells (MSC), to investigate whether MSCs migrate and/or remain at the site of injury, and to analyze the effects of MSCs on inflammation, astrocytic reactivity and activation of endogenous stem cells. Three hours after SCI, animals received bone marrow-derived MSCs (1×107 in 1mL PBS, IV). Animals were euthanized 24 hours, 7 and 21 days post-injury. The MSC were not present in the site of the lesion and the immunofluorescent evaluation showed significant attenuation of inflammatory response with reduction in macrophages labeled with anti-CD68 antibody (ED1), decreased immunoreactivity of astrocytes (GFAP+) and greater activation of endogenous stem cells (nestin+) in the treated groups. Therefore, cell transplantation have a positive effect on recovery from traumatic spinal cord injury possibly due to the potential of MSCs to attenuate the immune response.A lesão traumática da medula espinhal resulta em déficits neurológicos graves, em sua maioria irreversíveis. A terapia celular representa uma estratégia de tratamento principalmente com o uso de células-tronco com resultados satisfatórios em diversos modelos experimentais. O objetivo do estudo foi comparar o tratamento de lesão medular (LM) com e sem células-tronco mesenquimais (MSC), investigar se as MSCs migram e/ou permanecem no local da lesão e analisar os efeitos das MSCs na inflamação, reatividade astrocítica e ativação de células-tronco endógenas. Três horas após a SCI, os animais receberam MSCs derivadas da medula óssea (1×107 em 1mL de PBS, IV). Os animais foram eutanasiados 24 horas, 7 e 21 dias após a lesão. As CTMs não estavam presentes no local da lesão e a avaliação imunofluorescente mostrou atenuação significativa da resposta inflamatória com redução de macrófagos marcados com anticorpo anti-CD68 (ED1), diminuição da imunorreatividade dos astrócitos (GFAP+) e maior ativação de células-tronco endógenas. nestin+) nos grupos tratados. Portanto, o transplante de células tem um efeito positivo na recuperação da lesão medular traumática possivelmente devido ao potencial das CTMs em atenuar a resposta imuneengUniversidade Federal de Minas GeraisUFMGBrasilICB - INSTITUTO DE CIÊNCIAS BIOLOGICASSemina: Ciências AgráriasTerapia celularNeurocirurgiaCélula troncoCellular therapyNeurosurgeryStem cellMesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of ratsCélulas-tronco mesenquimais promovem resposta aumentada de células-tronco neurais endógenas na lesão medular de ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21596Marta Rocha AraújoPablo Herthel de CarvalhoTaís Silva de PaulaBárbara Silva OkanoRicardo Junqueira Del CarloRômulo Dias NovaesDaise Nunes Queiroz da CunhaClóvis Andrade Nevesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/41454/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINAL2016_Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats.pdf2016_Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats.pdfapplication/pdf1079391https://repositorio.ufmg.br/bitstream/1843/41454/2/2016_Mesenchymal%20stem%20cells%20promote%20augmented%20response%20of%20endogenous%20neural%20stem%20cells%20in%20spinal%20cord%20injury%20of%20rats.pdf3b63b9a5e634c9837875f6cfcb1cc903MD521843/414542022-05-06 19:59:25.993oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-05-06T22:59:25Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
dc.title.alternative.pt_BR.fl_str_mv |
Células-tronco mesenquimais promovem resposta aumentada de células-tronco neurais endógenas na lesão medular de ratos |
title |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
spellingShingle |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats Marta Rocha Araújo Cellular therapy Neurosurgery Stem cell Terapia celular Neurocirurgia Célula tronco |
title_short |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
title_full |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
title_fullStr |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
title_full_unstemmed |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
title_sort |
Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats |
author |
Marta Rocha Araújo |
author_facet |
Marta Rocha Araújo Pablo Herthel de Carvalho Taís Silva de Paula Bárbara Silva Okano Ricardo Junqueira Del Carlo Rômulo Dias Novaes Daise Nunes Queiroz da Cunha Clóvis Andrade Neves |
author_role |
author |
author2 |
Pablo Herthel de Carvalho Taís Silva de Paula Bárbara Silva Okano Ricardo Junqueira Del Carlo Rômulo Dias Novaes Daise Nunes Queiroz da Cunha Clóvis Andrade Neves |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Marta Rocha Araújo Pablo Herthel de Carvalho Taís Silva de Paula Bárbara Silva Okano Ricardo Junqueira Del Carlo Rômulo Dias Novaes Daise Nunes Queiroz da Cunha Clóvis Andrade Neves |
dc.subject.por.fl_str_mv |
Cellular therapy Neurosurgery Stem cell |
topic |
Cellular therapy Neurosurgery Stem cell Terapia celular Neurocirurgia Célula tronco |
dc.subject.other.pt_BR.fl_str_mv |
Terapia celular Neurocirurgia Célula tronco |
description |
Traumatic spinal cord injury results in severe neurological deficits, mostly irreversible. The cell therapy represents a strategy for treatment particularly with the use of stem cells with satisfactory results in several experimental models. The aim of the study was to compare the treatment of spinal cord injury (SCI) with and without mesenchymal stem cells (MSC), to investigate whether MSCs migrate and/or remain at the site of injury, and to analyze the effects of MSCs on inflammation, astrocytic reactivity and activation of endogenous stem cells. Three hours after SCI, animals received bone marrow-derived MSCs (1×107 in 1mL PBS, IV). Animals were euthanized 24 hours, 7 and 21 days post-injury. The MSC were not present in the site of the lesion and the immunofluorescent evaluation showed significant attenuation of inflammatory response with reduction in macrophages labeled with anti-CD68 antibody (ED1), decreased immunoreactivity of astrocytes (GFAP+) and greater activation of endogenous stem cells (nestin+) in the treated groups. Therefore, cell transplantation have a positive effect on recovery from traumatic spinal cord injury possibly due to the potential of MSCs to attenuate the immune response. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2022-05-06T22:59:25Z |
dc.date.available.fl_str_mv |
2022-05-06T22:59:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
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http://hdl.handle.net/1843/41454 |
dc.identifier.issn.pt_BR.fl_str_mv |
1676-546X |
identifier_str_mv |
1676-546X |
url |
http://hdl.handle.net/1843/41454 |
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eng |
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eng |
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Semina: Ciências Agrárias |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS |
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Universidade Federal de Minas Gerais |
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UFMG |
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