Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/36825 |
Resumo: | Cholesterol is an important component of cell plasma membrane. Due to its chemical composition (long rigid hydrophobic chain and a small polar hydroxyl group), it fits most of its structure into the lipid bilayer, where its steroid rings are in close proximity and attracted to the hydrocarbon chains of neighboring lipids. This gives a condensing effect on the packing of lipids in cell membranes creating cholesterol-enriched regions called membrane rafts, which also congregate a lot of specific proteins. Membrane rafts have been shown to work as platforms involved with signaling in diverse cellular processes, such as immune regulation, cell cycle control, membrane trafficking and fusion events. A series of studies in the last two decades have linked many of these functions with the effects of membrane cholesterol content and rafts integrity on actin cytoskeleton organization, as well as its consequences in cellular biomechanics. This was possible by using microscopy techniques before and after manipulation of cholesterol content from cell plasma membrane, using agents that are able to sequester these molecules, such as cyclodextrins. In this review we’ll give a personal perspective on these studies and how microscopy techniques were important to unravel the effects of cholesterol on actin and cellular mechanics. We will also discuss how actin and cholesterol contributes to control cell secretion and vesicular trafficking. |
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2021-07-21T13:51:07Z2021-07-21T13:51:07Z20165s1S101S11710.3233/BSI-16015722128808http://hdl.handle.net/1843/36825Cholesterol is an important component of cell plasma membrane. Due to its chemical composition (long rigid hydrophobic chain and a small polar hydroxyl group), it fits most of its structure into the lipid bilayer, where its steroid rings are in close proximity and attracted to the hydrocarbon chains of neighboring lipids. This gives a condensing effect on the packing of lipids in cell membranes creating cholesterol-enriched regions called membrane rafts, which also congregate a lot of specific proteins. Membrane rafts have been shown to work as platforms involved with signaling in diverse cellular processes, such as immune regulation, cell cycle control, membrane trafficking and fusion events. A series of studies in the last two decades have linked many of these functions with the effects of membrane cholesterol content and rafts integrity on actin cytoskeleton organization, as well as its consequences in cellular biomechanics. This was possible by using microscopy techniques before and after manipulation of cholesterol content from cell plasma membrane, using agents that are able to sequester these molecules, such as cyclodextrins. In this review we’ll give a personal perspective on these studies and how microscopy techniques were important to unravel the effects of cholesterol on actin and cellular mechanics. We will also discuss how actin and cholesterol contributes to control cell secretion and vesicular trafficking.O colesterol é um componente importante da membrana plasmática da célula. Devido à sua composição química (longa cadeia hidrofóbica rígida e um pequeno grupo hidroxila polar), ele se encaixa a maior parte de sua estrutura na bicamada lipídica, onde estão seus anéis esteróides em estreita proximidade e atraídos para as cadeias de hidrocarbonetos de lipídios vizinhos. Isso dá um efeito de condensação na embalagem de lipídios nas membranas celulares, criando regiões enriquecidas com colesterol chamadas jangadas de membrana, que também reúnem uma grande quantidade de proteínas. Já foi demonstrado que jangadas de membrana funcionam como plataformas envolvidas com a sinalização em diversos processos celulares, como regulação imunológica, controle do ciclo celular, tráfego de membrana e eventos de fusão. Uma série de estudos nas últimas duas décadas têm relacionou muitas dessas funções com os efeitos do conteúdo de colesterol da membrana e integridade das jangadas na organização do citoesqueleto de actina, bem como suas consequências na biomecânica celular. Isso foi possível usando técnicas de microscopia antes e após a manipulação do conteúdo de colesterol da membrana plasmática da célula, usando agentes que são capazes de sequestrar essas moléculas, como ciclodextrinas. Nesta revisão, daremos uma perspectiva pessoal sobre esses estudos e como as técnicas de microscopia eram importante para desvendar os efeitos do colesterol na actina e na mecânica celular. Também discutiremos como a actina e o colesterol contribui para o controle da secreção celular e do tráfico vesicular. Mais sobre o texto originalÉ necessário fornecer o texto original para ver mais informações sobre a tradução Enviar feedback Painéis lateraisporUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MORFOLOGIABiomedical spectroscopy and imagingColesterolBiomecânicaFisiologia celularcholesterolvesicular traffickingcell biomechanicsUnderstanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imagingCompreendendo o papel do colesterol na biomecânica celular e na regulação do tráfego vesicular: o poder da imageminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLuciana de Oliveira Andradeapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/36825/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALbsi_2016_5-S1_bsi-5-s1-bsi157_bsi-5-bsi157.pdfbsi_2016_5-S1_bsi-5-s1-bsi157_bsi-5-bsi157.pdfapplication/pdf828448https://repositorio.ufmg.br/bitstream/1843/36825/2/bsi_2016_5-S1_bsi-5-s1-bsi157_bsi-5-bsi157.pdfa792a024f94b4af53c9aff8af60a1a0bMD521843/368252021-07-21 10:51:07.398oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2021-07-21T13:51:07Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging |
dc.title.alternative.pt_BR.fl_str_mv |
Compreendendo o papel do colesterol na biomecânica celular e na regulação do tráfego vesicular: o poder da imagem |
title |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging |
spellingShingle |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging Luciana de Oliveira Andrade cholesterol vesicular trafficking cell biomechanics Colesterol Biomecânica Fisiologia celular |
title_short |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging |
title_full |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging |
title_fullStr |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging |
title_full_unstemmed |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging |
title_sort |
Understanding the role of cholesterol in cellular biomechanics and regulation of vesicular trafficking: the power of imaging |
author |
Luciana de Oliveira Andrade |
author_facet |
Luciana de Oliveira Andrade |
author_role |
author |
dc.contributor.author.fl_str_mv |
Luciana de Oliveira Andrade |
dc.subject.por.fl_str_mv |
cholesterol vesicular trafficking cell biomechanics |
topic |
cholesterol vesicular trafficking cell biomechanics Colesterol Biomecânica Fisiologia celular |
dc.subject.other.pt_BR.fl_str_mv |
Colesterol Biomecânica Fisiologia celular |
description |
Cholesterol is an important component of cell plasma membrane. Due to its chemical composition (long rigid hydrophobic chain and a small polar hydroxyl group), it fits most of its structure into the lipid bilayer, where its steroid rings are in close proximity and attracted to the hydrocarbon chains of neighboring lipids. This gives a condensing effect on the packing of lipids in cell membranes creating cholesterol-enriched regions called membrane rafts, which also congregate a lot of specific proteins. Membrane rafts have been shown to work as platforms involved with signaling in diverse cellular processes, such as immune regulation, cell cycle control, membrane trafficking and fusion events. A series of studies in the last two decades have linked many of these functions with the effects of membrane cholesterol content and rafts integrity on actin cytoskeleton organization, as well as its consequences in cellular biomechanics. This was possible by using microscopy techniques before and after manipulation of cholesterol content from cell plasma membrane, using agents that are able to sequester these molecules, such as cyclodextrins. In this review we’ll give a personal perspective on these studies and how microscopy techniques were important to unravel the effects of cholesterol on actin and cellular mechanics. We will also discuss how actin and cholesterol contributes to control cell secretion and vesicular trafficking. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2021-07-21T13:51:07Z |
dc.date.available.fl_str_mv |
2021-07-21T13:51:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/36825 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.3233/BSI-160157 |
dc.identifier.issn.pt_BR.fl_str_mv |
22128808 |
identifier_str_mv |
10.3233/BSI-160157 22128808 |
url |
http://hdl.handle.net/1843/36825 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Biomedical spectroscopy and imaging |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE MORFOLOGIA |
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Universidade Federal de Minas Gerais |
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