Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model

Detalhes bibliográficos
Autor(a) principal: Warley Gomes Dos Santos
Data de Publicação: 2019
Outros Autores: Tatiana L. Silveira, Aparecida Tatiane Fiúza, Ana Flávia Machado Botelho, Ivy Nayra N. Gonçalves, Ênio Ferreira, Benito Soto-blanco, Marília Martins Melo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1590/1678-4162-10105
http://hdl.handle.net/1843/56437
https://orcid.org/0000-0001-5204-0512
https://orcid.org/0000-0002-1835-0303
https://orcid.org/0000-0003-3861-1838
https://orcid.org/0000-0003-3630-6776
Resumo: This study aims to report the occurrence of in vivo apoptosis induced by Bothrops alternatus snake venom in skeletal muscle cells. Five adult male rabbits received 150μg/kg of venom in the vastus lateralis muscle, while another five animals received 0.1% BSA diluted in PBS in the same place. After 12 hours, the animals were euthanized, and samples from the inoculation site were collected for histopathological analysis. Necrosis and hemorrhage were evidenced in the muscle cells. In addition, immunohistochemical analysis to identify activated caspase-3 revealed granular and aggregated markings in the cytoplasm of muscle cells, compatible with the apoptosis process. This is the first report confirming that B. alternatus venom causes apoptosis in vivo in skeletal muscle cells.
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spelling 2023-07-17T18:50:46Z2023-07-17T18:50:46Z2019711349352https://doi.org/10.1590/1678-4162-101051678-4162http://hdl.handle.net/1843/56437https://orcid.org/0000-0001-5204-0512https://orcid.org/0000-0002-1835-0303https://orcid.org/0000-0003-3861-1838https://orcid.org/0000-0003-3630-6776This study aims to report the occurrence of in vivo apoptosis induced by Bothrops alternatus snake venom in skeletal muscle cells. Five adult male rabbits received 150μg/kg of venom in the vastus lateralis muscle, while another five animals received 0.1% BSA diluted in PBS in the same place. After 12 hours, the animals were euthanized, and samples from the inoculation site were collected for histopathological analysis. Necrosis and hemorrhage were evidenced in the muscle cells. In addition, immunohistochemical analysis to identify activated caspase-3 revealed granular and aggregated markings in the cytoplasm of muscle cells, compatible with the apoptosis process. This is the first report confirming that B. alternatus venom causes apoptosis in vivo in skeletal muscle cells.Este estudo objetiva reportar a ocorrência de apoptose in vivo induzida pelo veneno da serpente Bothrops alternatus em células musculares esqueléticas. Cinco coelhos machos, adultos, receberam 150μg/kg de veneno no músculo vasto lateral, enquanto outros cinco animais receberam 0,1% de BSA diluído em PBS no mesmo local. Após 12 horas, os animais foram eutanasiados, e amostras do local de inoculação foram coletadas para análise histopatológica. Foram evidenciadas necrose e hemorragia nas células musculares. Além disso, a análise imuno-histoquímica para identificação de caspase-3 ativada revelou marcações granulares e agregadas no citoplasma das células musculares, compatíveis com o processo de apoptose. Este é o primeiro relato que confirma o veneno de B. alternatus como causador de apoptose in vivo em células musculares esqueléticas.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoengUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE PATOLOGIAVET - DEPARTAMENTO DE CLÍNICA E CIRURGIAArquivo Brasileiro de Medicina Veterinária e ZootecniaVenenos de SerpentesApoptoseToxicologiaCoelhosSnake venomBotropic venomApoptosisToxicologyRabbitBothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit modelO veneno da serpente Bothrops alternatus induz apoptose nas células musculares de coelhosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/abmvz/a/LJjGKG7Y3cgvhHTs3qF6MFr/?lang=en#Warley Gomes Dos SantosTatiana L. SilveiraAparecida Tatiane FiúzaAna Flávia Machado BotelhoIvy Nayra N. GonçalvesÊnio FerreiraBenito Soto-blancoMarília Martins Meloapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56437/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALBothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model.pdfBothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model.pdfapplication/pdf263536https://repositorio.ufmg.br/bitstream/1843/56437/2/Bothrops%20alternatus%20snake%20venom%20induces%20apoptosis%20of%20skeletal%20muscle%20cells%20in%20a%20rabbit%20model.pdf7c903fde1ae85ad59b2528170133cde6MD521843/564372023-07-17 15:50:46.168oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-17T18:50:46Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
dc.title.alternative.pt_BR.fl_str_mv O veneno da serpente Bothrops alternatus induz apoptose nas células musculares de coelhos
title Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
spellingShingle Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
Warley Gomes Dos Santos
Snake venom
Botropic venom
Apoptosis
Toxicology
Rabbit
Venenos de Serpentes
Apoptose
Toxicologia
Coelhos
title_short Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
title_full Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
title_fullStr Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
title_full_unstemmed Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
title_sort Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
author Warley Gomes Dos Santos
author_facet Warley Gomes Dos Santos
Tatiana L. Silveira
Aparecida Tatiane Fiúza
Ana Flávia Machado Botelho
Ivy Nayra N. Gonçalves
Ênio Ferreira
Benito Soto-blanco
Marília Martins Melo
author_role author
author2 Tatiana L. Silveira
Aparecida Tatiane Fiúza
Ana Flávia Machado Botelho
Ivy Nayra N. Gonçalves
Ênio Ferreira
Benito Soto-blanco
Marília Martins Melo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Warley Gomes Dos Santos
Tatiana L. Silveira
Aparecida Tatiane Fiúza
Ana Flávia Machado Botelho
Ivy Nayra N. Gonçalves
Ênio Ferreira
Benito Soto-blanco
Marília Martins Melo
dc.subject.por.fl_str_mv Snake venom
Botropic venom
Apoptosis
Toxicology
Rabbit
topic Snake venom
Botropic venom
Apoptosis
Toxicology
Rabbit
Venenos de Serpentes
Apoptose
Toxicologia
Coelhos
dc.subject.other.pt_BR.fl_str_mv Venenos de Serpentes
Apoptose
Toxicologia
Coelhos
description This study aims to report the occurrence of in vivo apoptosis induced by Bothrops alternatus snake venom in skeletal muscle cells. Five adult male rabbits received 150μg/kg of venom in the vastus lateralis muscle, while another five animals received 0.1% BSA diluted in PBS in the same place. After 12 hours, the animals were euthanized, and samples from the inoculation site were collected for histopathological analysis. Necrosis and hemorrhage were evidenced in the muscle cells. In addition, immunohistochemical analysis to identify activated caspase-3 revealed granular and aggregated markings in the cytoplasm of muscle cells, compatible with the apoptosis process. This is the first report confirming that B. alternatus venom causes apoptosis in vivo in skeletal muscle cells.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2023-07-17T18:50:46Z
dc.date.available.fl_str_mv 2023-07-17T18:50:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/56437
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1590/1678-4162-10105
dc.identifier.issn.pt_BR.fl_str_mv 1678-4162
dc.identifier.orcid.pt_BR.fl_str_mv https://orcid.org/0000-0001-5204-0512
https://orcid.org/0000-0002-1835-0303
https://orcid.org/0000-0003-3861-1838
https://orcid.org/0000-0003-3630-6776
url https://doi.org/10.1590/1678-4162-10105
http://hdl.handle.net/1843/56437
https://orcid.org/0000-0001-5204-0512
https://orcid.org/0000-0002-1835-0303
https://orcid.org/0000-0003-3861-1838
https://orcid.org/0000-0003-3630-6776
identifier_str_mv 1678-4162
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICB - DEPARTAMENTO DE PATOLOGIA
VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
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https://repositorio.ufmg.br/bitstream/1843/56437/2/Bothrops%20alternatus%20snake%20venom%20induces%20apoptosis%20of%20skeletal%20muscle%20cells%20in%20a%20rabbit%20model.pdf
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