Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/1678-4162-10105 http://hdl.handle.net/1843/56437 https://orcid.org/0000-0001-5204-0512 https://orcid.org/0000-0002-1835-0303 https://orcid.org/0000-0003-3861-1838 https://orcid.org/0000-0003-3630-6776 |
Resumo: | This study aims to report the occurrence of in vivo apoptosis induced by Bothrops alternatus snake venom in skeletal muscle cells. Five adult male rabbits received 150μg/kg of venom in the vastus lateralis muscle, while another five animals received 0.1% BSA diluted in PBS in the same place. After 12 hours, the animals were euthanized, and samples from the inoculation site were collected for histopathological analysis. Necrosis and hemorrhage were evidenced in the muscle cells. In addition, immunohistochemical analysis to identify activated caspase-3 revealed granular and aggregated markings in the cytoplasm of muscle cells, compatible with the apoptosis process. This is the first report confirming that B. alternatus venom causes apoptosis in vivo in skeletal muscle cells. |
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2023-07-17T18:50:46Z2023-07-17T18:50:46Z2019711349352https://doi.org/10.1590/1678-4162-101051678-4162http://hdl.handle.net/1843/56437https://orcid.org/0000-0001-5204-0512https://orcid.org/0000-0002-1835-0303https://orcid.org/0000-0003-3861-1838https://orcid.org/0000-0003-3630-6776This study aims to report the occurrence of in vivo apoptosis induced by Bothrops alternatus snake venom in skeletal muscle cells. Five adult male rabbits received 150μg/kg of venom in the vastus lateralis muscle, while another five animals received 0.1% BSA diluted in PBS in the same place. After 12 hours, the animals were euthanized, and samples from the inoculation site were collected for histopathological analysis. Necrosis and hemorrhage were evidenced in the muscle cells. In addition, immunohistochemical analysis to identify activated caspase-3 revealed granular and aggregated markings in the cytoplasm of muscle cells, compatible with the apoptosis process. This is the first report confirming that B. alternatus venom causes apoptosis in vivo in skeletal muscle cells.Este estudo objetiva reportar a ocorrência de apoptose in vivo induzida pelo veneno da serpente Bothrops alternatus em células musculares esqueléticas. Cinco coelhos machos, adultos, receberam 150μg/kg de veneno no músculo vasto lateral, enquanto outros cinco animais receberam 0,1% de BSA diluído em PBS no mesmo local. Após 12 horas, os animais foram eutanasiados, e amostras do local de inoculação foram coletadas para análise histopatológica. Foram evidenciadas necrose e hemorragia nas células musculares. Além disso, a análise imuno-histoquímica para identificação de caspase-3 ativada revelou marcações granulares e agregadas no citoplasma das células musculares, compatíveis com o processo de apoptose. Este é o primeiro relato que confirma o veneno de B. alternatus como causador de apoptose in vivo em células musculares esqueléticas.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoengUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE PATOLOGIAVET - DEPARTAMENTO DE CLÍNICA E CIRURGIAArquivo Brasileiro de Medicina Veterinária e ZootecniaVenenos de SerpentesApoptoseToxicologiaCoelhosSnake venomBotropic venomApoptosisToxicologyRabbitBothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit modelO veneno da serpente Bothrops alternatus induz apoptose nas células musculares de coelhosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/abmvz/a/LJjGKG7Y3cgvhHTs3qF6MFr/?lang=en#Warley Gomes Dos SantosTatiana L. SilveiraAparecida Tatiane FiúzaAna Flávia Machado BotelhoIvy Nayra N. GonçalvesÊnio FerreiraBenito Soto-blancoMarília Martins Meloapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56437/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALBothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model.pdfBothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model.pdfapplication/pdf263536https://repositorio.ufmg.br/bitstream/1843/56437/2/Bothrops%20alternatus%20snake%20venom%20induces%20apoptosis%20of%20skeletal%20muscle%20cells%20in%20a%20rabbit%20model.pdf7c903fde1ae85ad59b2528170133cde6MD521843/564372023-07-17 15:50:46.168oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-17T18:50:46Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model |
dc.title.alternative.pt_BR.fl_str_mv |
O veneno da serpente Bothrops alternatus induz apoptose nas células musculares de coelhos |
title |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model |
spellingShingle |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model Warley Gomes Dos Santos Snake venom Botropic venom Apoptosis Toxicology Rabbit Venenos de Serpentes Apoptose Toxicologia Coelhos |
title_short |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model |
title_full |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model |
title_fullStr |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model |
title_full_unstemmed |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model |
title_sort |
Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model |
author |
Warley Gomes Dos Santos |
author_facet |
Warley Gomes Dos Santos Tatiana L. Silveira Aparecida Tatiane Fiúza Ana Flávia Machado Botelho Ivy Nayra N. Gonçalves Ênio Ferreira Benito Soto-blanco Marília Martins Melo |
author_role |
author |
author2 |
Tatiana L. Silveira Aparecida Tatiane Fiúza Ana Flávia Machado Botelho Ivy Nayra N. Gonçalves Ênio Ferreira Benito Soto-blanco Marília Martins Melo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Warley Gomes Dos Santos Tatiana L. Silveira Aparecida Tatiane Fiúza Ana Flávia Machado Botelho Ivy Nayra N. Gonçalves Ênio Ferreira Benito Soto-blanco Marília Martins Melo |
dc.subject.por.fl_str_mv |
Snake venom Botropic venom Apoptosis Toxicology Rabbit |
topic |
Snake venom Botropic venom Apoptosis Toxicology Rabbit Venenos de Serpentes Apoptose Toxicologia Coelhos |
dc.subject.other.pt_BR.fl_str_mv |
Venenos de Serpentes Apoptose Toxicologia Coelhos |
description |
This study aims to report the occurrence of in vivo apoptosis induced by Bothrops alternatus snake venom in skeletal muscle cells. Five adult male rabbits received 150μg/kg of venom in the vastus lateralis muscle, while another five animals received 0.1% BSA diluted in PBS in the same place. After 12 hours, the animals were euthanized, and samples from the inoculation site were collected for histopathological analysis. Necrosis and hemorrhage were evidenced in the muscle cells. In addition, immunohistochemical analysis to identify activated caspase-3 revealed granular and aggregated markings in the cytoplasm of muscle cells, compatible with the apoptosis process. This is the first report confirming that B. alternatus venom causes apoptosis in vivo in skeletal muscle cells. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2023-07-17T18:50:46Z |
dc.date.available.fl_str_mv |
2023-07-17T18:50:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56437 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/1678-4162-10105 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-4162 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0001-5204-0512 https://orcid.org/0000-0002-1835-0303 https://orcid.org/0000-0003-3861-1838 https://orcid.org/0000-0003-3630-6776 |
url |
https://doi.org/10.1590/1678-4162-10105 http://hdl.handle.net/1843/56437 https://orcid.org/0000-0001-5204-0512 https://orcid.org/0000-0002-1835-0303 https://orcid.org/0000-0003-3861-1838 https://orcid.org/0000-0003-3630-6776 |
identifier_str_mv |
1678-4162 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE PATOLOGIA VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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