Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.3389/fmicb.2017.01681 http://hdl.handle.net/1843/56305 https://orcid.org/0000-0002-0604-8510 https://orcid.org/0000-0001-9669-7890 https://orcid.org/0000-0002-6176-4069 https://orcid.org/0000-0003-0381-9225 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0001-9540-8946 |
Resumo: | The intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops. To evaluate the safety of fungal biological agents for human health, tests to detect potentially adverse effects, such as allergenicity, toxicity, infectivity and pathogenicity, are crucial. In addition, identifying possible immunomodulating properties of fungal biocontrol agents merits further investigation. Thus, the aim of this study was to evaluate the effects of T. asperelloides spores in the internalization of Candida parapsilosis yeast by mice phagocytes, in order to elucidate the cellular and molecular mechanism of this interaction, as a model to understand possible in vivo effects of this fungus. For this, mice were exposed to a fungal spore suspension through-intraperitoneal injection, euthanized and cells from the peripheral blood and peritoneal cavity were collected for functional, quantitative and phenotypic analysis, throughout analysis of membrane receptors gene expression, phagocytosis ability and cells immunophenotyping M1 (CCR7 and CD86) and M2 (CCR2 and CD206). Our analyses showed that phagocytes exposed to fungal spores had reduced phagocytic capacity, as well as a decrease in the quantity of neutrophils and monocytes in the peripheral blood and peritoneal cavity. Moreover, macrophages exposed to T. asperelloides spores did not display the phenotypic profile M1/M2, and had reduced expression of pattern recognition receptors, such as TLR2, dectin-1 and dectin-2, all involved in the first line of defense against clinically important yeasts. Our data could infer that T. asperelloides spores may confer susceptibility to infection by C. parapsilosis. |
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2023-07-14T20:38:14Z2023-07-14T20:38:14Z20178https://doi.org/10.3389/fmicb.2017.016811664-302Xhttp://hdl.handle.net/1843/56305https://orcid.org/0000-0002-0604-8510https://orcid.org/0000-0001-9669-7890https://orcid.org/0000-0002-6176-4069https://orcid.org/0000-0003-0381-9225https://orcid.org/0000-0002-1108-5666https://orcid.org/0000-0001-9540-8946The intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops. To evaluate the safety of fungal biological agents for human health, tests to detect potentially adverse effects, such as allergenicity, toxicity, infectivity and pathogenicity, are crucial. In addition, identifying possible immunomodulating properties of fungal biocontrol agents merits further investigation. Thus, the aim of this study was to evaluate the effects of T. asperelloides spores in the internalization of Candida parapsilosis yeast by mice phagocytes, in order to elucidate the cellular and molecular mechanism of this interaction, as a model to understand possible in vivo effects of this fungus. For this, mice were exposed to a fungal spore suspension through-intraperitoneal injection, euthanized and cells from the peripheral blood and peritoneal cavity were collected for functional, quantitative and phenotypic analysis, throughout analysis of membrane receptors gene expression, phagocytosis ability and cells immunophenotyping M1 (CCR7 and CD86) and M2 (CCR2 and CD206). Our analyses showed that phagocytes exposed to fungal spores had reduced phagocytic capacity, as well as a decrease in the quantity of neutrophils and monocytes in the peripheral blood and peritoneal cavity. Moreover, macrophages exposed to T. asperelloides spores did not display the phenotypic profile M1/M2, and had reduced expression of pattern recognition receptors, such as TLR2, dectin-1 and dectin-2, all involved in the first line of defense against clinically important yeasts. Our data could infer that T. asperelloides spores may confer susceptibility to infection by C. parapsilosis.O uso intensivo de agrotóxicos para o controle de pragas na agricultura tem promovido diversos problemas relacionados ao meio ambiente. Como os pesticidas químicos permanecem controversos, os agentes de biocontrole originários de fungos podem ser uma alternativa. Dentre eles, destacam-se os agentes de biocontrole derivados dos fungos do gênero Trichoderma, que têm sido documentados na limitação do crescimento de outros fungos fitopatogênicos nas raízes e folhas de diversas espécies vegetais. Um membro importante desse gênero é o Trichoderma asperelloides, cujos agentes de biocontrole têm sido usados para promover o crescimento de plantas e, ao mesmo tempo, tratar doenças do solo causadas por microrganismos em estufas e cultivos ao ar livre. Para avaliar a segurança de agentes biológicos fúngicos para a saúde humana, testes para detectar efeitos potencialmente adversos, como alergenicidade, toxicidade, infectividade e patogenicidade, são cruciais. Além disso, a identificação de possíveis propriedades imunomoduladoras de agentes de biocontrole fúngico merece uma investigação mais aprofundada. Assim, o objetivo deste estudo foi avaliar os efeitos dos esporos de T. asperelloides na internalização da levedura Candida parapsilosis por fagócitos de camundongos, a fim de elucidar o mecanismo celular e molecular dessa interação, como um modelo para compreender possíveis efeitos in vivo deste fungo. Para isso, camundongos foram expostos a uma suspensão de esporos fúngicos por meio de injeção intraperitoneal, eutanasiados e células do sangue periférico e da cavidade peritoneal foram coletadas para análise funcional, quantitativa e fenotípica, através da análise da expressão gênica de receptores de membrana, capacidade de fagocitose e imunofenotipagem de células M1 (CCR7 e CD86) e M2 (CCR2 e CD206). Nossas análises mostraram que fagócitos expostos a esporos fúngicos apresentaram redução da capacidade fagocitária, bem como diminuição da quantidade de neutrófilos e monócitos no sangue periférico e na cavidade peritoneal. Além disso, macrófagos expostos a esporos de T. asperelloides não apresentaram o perfil fenotípico M1/M2, e tiveram expressão reduzida de receptores de reconhecimento de padrões, como TLR2, dectina-1 e dectina-2, todos envolvidos na primeira linha de defesa contra infecções clínicas leveduras importantes. Nossos dados podem inferir que esporos de T. asperelloides podem conferir suscetibilidade à infecção por C. parapsilosis.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorengUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIAICB - DEPARTAMENTO DE MICROBIOLOGIAFrontiers in MicrobiologyAgentes de controle biológicoFagocitoseEsporos fúngicosCandida parapsilosisBiocontrol agentPhagocytosisConidiaPRRCandida parapsilosisTrichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarizationEsporos de Trichoderma asperelloides regulam negativamente os receptores dectina1/2 e TLR2 de macrófagos de camundongos e diminuem a fagocitose de Candida parapsilosis independente da polarização M1/M2info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.frontiersin.org/articles/10.3389/fmicb.2017.01681/fullAndréa Gonçalves dos SantosÉrica Araújo MendesRafael P. de OliveiraAna Maria Caetano FariaAurizangela O. de SousaCarlos Priminho PirovaniFernanda F. de AraújoAndréa T. de CarvalhoMarliete Carvalho CostaDaniel de Assis SantosQuimi V. MontoyaAndre RodriguesJane Lima Santosapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56305/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALTrichoderma asperelloides spores downregulate dectin1_2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1_M2 polarization.pdfTrichoderma asperelloides spores downregulate dectin1_2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1_M2 polarization.pdfapplication/pdf2710724https://repositorio.ufmg.br/bitstream/1843/56305/2/Trichoderma%20asperelloides%20spores%20downregulate%20dectin1_2%20and%20TLR2%20receptors%20of%20mice%20macrophages%20and%20decrease%20Candida%20parapsilosis%20phagocytosis%20independent%20of%20the%20M1_M2%20polarization.pdff31e8c84fb2f9ff33a403d3a7c5c2f68MD521843/563052023-07-14 17:38:14.745oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T20:38:14Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization |
dc.title.alternative.pt_BR.fl_str_mv |
Esporos de Trichoderma asperelloides regulam negativamente os receptores dectina1/2 e TLR2 de macrófagos de camundongos e diminuem a fagocitose de Candida parapsilosis independente da polarização M1/M2 |
title |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization |
spellingShingle |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization Andréa Gonçalves dos Santos Biocontrol agent Phagocytosis Conidia PRR Candida parapsilosis Agentes de controle biológico Fagocitose Esporos fúngicos Candida parapsilosis |
title_short |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization |
title_full |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization |
title_fullStr |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization |
title_full_unstemmed |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization |
title_sort |
Trichoderma asperelloides spores downregulate dectin1/2 and TLR2 receptors of mice macrophages and decrease Candida parapsilosis phagocytosis independent of the M1/M2 polarization |
author |
Andréa Gonçalves dos Santos |
author_facet |
Andréa Gonçalves dos Santos Érica Araújo Mendes Rafael P. de Oliveira Ana Maria Caetano Faria Aurizangela O. de Sousa Carlos Priminho Pirovani Fernanda F. de Araújo Andréa T. de Carvalho Marliete Carvalho Costa Daniel de Assis Santos Quimi V. Montoya Andre Rodrigues Jane Lima Santos |
author_role |
author |
author2 |
Érica Araújo Mendes Rafael P. de Oliveira Ana Maria Caetano Faria Aurizangela O. de Sousa Carlos Priminho Pirovani Fernanda F. de Araújo Andréa T. de Carvalho Marliete Carvalho Costa Daniel de Assis Santos Quimi V. Montoya Andre Rodrigues Jane Lima Santos |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Andréa Gonçalves dos Santos Érica Araújo Mendes Rafael P. de Oliveira Ana Maria Caetano Faria Aurizangela O. de Sousa Carlos Priminho Pirovani Fernanda F. de Araújo Andréa T. de Carvalho Marliete Carvalho Costa Daniel de Assis Santos Quimi V. Montoya Andre Rodrigues Jane Lima Santos |
dc.subject.por.fl_str_mv |
Biocontrol agent Phagocytosis Conidia PRR Candida parapsilosis |
topic |
Biocontrol agent Phagocytosis Conidia PRR Candida parapsilosis Agentes de controle biológico Fagocitose Esporos fúngicos Candida parapsilosis |
dc.subject.other.pt_BR.fl_str_mv |
Agentes de controle biológico Fagocitose Esporos fúngicos Candida parapsilosis |
description |
The intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops. To evaluate the safety of fungal biological agents for human health, tests to detect potentially adverse effects, such as allergenicity, toxicity, infectivity and pathogenicity, are crucial. In addition, identifying possible immunomodulating properties of fungal biocontrol agents merits further investigation. Thus, the aim of this study was to evaluate the effects of T. asperelloides spores in the internalization of Candida parapsilosis yeast by mice phagocytes, in order to elucidate the cellular and molecular mechanism of this interaction, as a model to understand possible in vivo effects of this fungus. For this, mice were exposed to a fungal spore suspension through-intraperitoneal injection, euthanized and cells from the peripheral blood and peritoneal cavity were collected for functional, quantitative and phenotypic analysis, throughout analysis of membrane receptors gene expression, phagocytosis ability and cells immunophenotyping M1 (CCR7 and CD86) and M2 (CCR2 and CD206). Our analyses showed that phagocytes exposed to fungal spores had reduced phagocytic capacity, as well as a decrease in the quantity of neutrophils and monocytes in the peripheral blood and peritoneal cavity. Moreover, macrophages exposed to T. asperelloides spores did not display the phenotypic profile M1/M2, and had reduced expression of pattern recognition receptors, such as TLR2, dectin-1 and dectin-2, all involved in the first line of defense against clinically important yeasts. Our data could infer that T. asperelloides spores may confer susceptibility to infection by C. parapsilosis. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2023-07-14T20:38:14Z |
dc.date.available.fl_str_mv |
2023-07-14T20:38:14Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56305 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.3389/fmicb.2017.01681 |
dc.identifier.issn.pt_BR.fl_str_mv |
1664-302X |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-0604-8510 https://orcid.org/0000-0001-9669-7890 https://orcid.org/0000-0002-6176-4069 https://orcid.org/0000-0003-0381-9225 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0001-9540-8946 |
url |
https://doi.org/10.3389/fmicb.2017.01681 http://hdl.handle.net/1843/56305 https://orcid.org/0000-0002-0604-8510 https://orcid.org/0000-0001-9669-7890 https://orcid.org/0000-0002-6176-4069 https://orcid.org/0000-0003-0381-9225 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0001-9540-8946 |
identifier_str_mv |
1664-302X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in Microbiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE MICROBIOLOGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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