ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/0103-8478cr20141203 http://hdl.handle.net/1843/58657 https://orcid.org/0000-0003-0603-0342 https://orcid.org/0000-0003-0795-5320 https://orcid.org/0000-0001-7819-4253 https://orcid.org/0000-0001-8524-3949 https://orcid.org/0000-0003-3630-6776 https://orcid.org/0000-0002-6082-930X https://orcid.org/0000-0002-8820-1063 |
Resumo: | This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats. |
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2023-09-14T00:34:02Z2023-09-14T00:34:02Z2016461150156https://doi.org/10.1590/0103-8478cr201412031678-4596http://hdl.handle.net/1843/58657https://orcid.org/0000-0003-0603-0342https://orcid.org/0000-0003-0795-5320https://orcid.org/0000-0001-7819-4253https://orcid.org/0000-0001-8524-3949https://orcid.org/0000-0003-3630-6776https://orcid.org/0000-0002-6082-930Xhttps://orcid.org/0000-0002-8820-1063This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats.Objetivou-se investigar o efeito neuroprotetor da aplicação intralesional da MVIIA em ratos submetidos ao trauma medular. Foram utilizados ratos Wistar, machos, com peso entre 300g±23.4, distribuídos em cinco grupos: controle negativo (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA e 20µM MVIIA. Após a laminectomia da vértebra torácica 12 (SHAM), os grupos PLA, 5µM MVIIA, 10µM MVIIA e 20µM MVIIA foram submetidos ao trauma medular agudo compressivo por cinco minutos e, cinco minutos após o trauma, receberam o tratamento específico em volume total padrão de 2µL, pela via intralesional, sendo utilizado como placebo o PBS estéril. A atividade locomotora foi avaliada pela escala proposta por Basso Beattie Bresnahan (BBB), com intuito de mostrar a padronização do trauma medular. Com 48 horas do trauma, os animais foram submetidos à eutanásia, coletou-se amostra do líquor no espaço atlantooccipital e um segmento medular, incluindo o epicentro e região caudal à lesão. Foram realizados ensaios de viabilidade mitocondrial, dosagem de glutamato, produção de espécies reativas de oxigênio (ERO) e peroxidação lipídica (PL). O delineamento do estudo foi inteiramente casualizado e os dados submetidos ao ANOVA, com comparação de médias pelo teste de SNK e os dados do teste BBB foram comparados utilizando o teste Kruskal-Wallis (P<0.05). Em relação aos escores do BBB, não houve diferença entre os grupos. A MVIIA não promoveu a diminuição dos níveis do glutamato, ERO, PL e não preservou a mitocôndria na aplicação intralesional, cinco minutos após o trauma medular em ratos.porUniversidade Federal de Minas GeraisUFMGBrasilVET - DEPARTAMENTO DE CLÍNICA E CIRURGIACiência RuralÔmega-ConotoxinasCaramujo ConusSobrevivência celularGlutamato desidrogenaseEspécies reativas de oxigênioPeroxidação de lipídeosMVIIACone snailCell viabilityGlutamateReactive oxygen speciesLipid peroxidationω-conotoxin MVIIA intralesional injection in spinal cord injury in ratsAplicação intralesional da ω-conotoxina MVIIA no trauma medular em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/cr/a/BCLHDTPsFVjy7q5tzzFQKtG/?lang=en#Karen Maciel de oliveiraNancy Scardua BindaMário Sérgio Lima LavorCarla Maria Osório SilvaIsabel Rodrigues RosadoTatiana Malagoli TaguchiEndrigo Gabellini Leonel AlvesMarília Martins MeloMarcus Vinícius GomezEliane Gonçalves de Meloapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/58657/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALω-conotoxin MVIIA intralesional injection in spinal cord injury in rats.pdfω-conotoxin MVIIA intralesional injection in spinal cord injury in rats.pdfapplication/pdf301407https://repositorio.ufmg.br/bitstream/1843/58657/2/%cf%89-conotoxin%20MVIIA%20intralesional%20injection%20in%20spinal%20cord%20injury%20in%20rats.pdf4fbd4ec07690cf1cc2834934f7b162e4MD521843/586572023-09-13 21:34:02.89oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-09-14T00:34:02Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
dc.title.alternative.pt_BR.fl_str_mv |
Aplicação intralesional da ω-conotoxina MVIIA no trauma medular em ratos |
title |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
spellingShingle |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats Karen Maciel de oliveira MVIIA Cone snail Cell viability Glutamate Reactive oxygen species Lipid peroxidation Ômega-Conotoxinas Caramujo Conus Sobrevivência celular Glutamato desidrogenase Espécies reativas de oxigênio Peroxidação de lipídeos |
title_short |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_full |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_fullStr |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_full_unstemmed |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_sort |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
author |
Karen Maciel de oliveira |
author_facet |
Karen Maciel de oliveira Nancy Scardua Binda Mário Sérgio Lima Lavor Carla Maria Osório Silva Isabel Rodrigues Rosado Tatiana Malagoli Taguchi Endrigo Gabellini Leonel Alves Marília Martins Melo Marcus Vinícius Gomez Eliane Gonçalves de Melo |
author_role |
author |
author2 |
Nancy Scardua Binda Mário Sérgio Lima Lavor Carla Maria Osório Silva Isabel Rodrigues Rosado Tatiana Malagoli Taguchi Endrigo Gabellini Leonel Alves Marília Martins Melo Marcus Vinícius Gomez Eliane Gonçalves de Melo |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Karen Maciel de oliveira Nancy Scardua Binda Mário Sérgio Lima Lavor Carla Maria Osório Silva Isabel Rodrigues Rosado Tatiana Malagoli Taguchi Endrigo Gabellini Leonel Alves Marília Martins Melo Marcus Vinícius Gomez Eliane Gonçalves de Melo |
dc.subject.por.fl_str_mv |
MVIIA Cone snail Cell viability Glutamate Reactive oxygen species Lipid peroxidation |
topic |
MVIIA Cone snail Cell viability Glutamate Reactive oxygen species Lipid peroxidation Ômega-Conotoxinas Caramujo Conus Sobrevivência celular Glutamato desidrogenase Espécies reativas de oxigênio Peroxidação de lipídeos |
dc.subject.other.pt_BR.fl_str_mv |
Ômega-Conotoxinas Caramujo Conus Sobrevivência celular Glutamato desidrogenase Espécies reativas de oxigênio Peroxidação de lipídeos |
description |
This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2023-09-14T00:34:02Z |
dc.date.available.fl_str_mv |
2023-09-14T00:34:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/58657 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/0103-8478cr20141203 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-4596 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0003-0603-0342 https://orcid.org/0000-0003-0795-5320 https://orcid.org/0000-0001-7819-4253 https://orcid.org/0000-0001-8524-3949 https://orcid.org/0000-0003-3630-6776 https://orcid.org/0000-0002-6082-930X https://orcid.org/0000-0002-8820-1063 |
url |
https://doi.org/10.1590/0103-8478cr20141203 http://hdl.handle.net/1843/58657 https://orcid.org/0000-0003-0603-0342 https://orcid.org/0000-0003-0795-5320 https://orcid.org/0000-0001-7819-4253 https://orcid.org/0000-0001-8524-3949 https://orcid.org/0000-0003-3630-6776 https://orcid.org/0000-0002-6082-930X https://orcid.org/0000-0002-8820-1063 |
identifier_str_mv |
1678-4596 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Ciência Rural |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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