Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/54842 |
Resumo: | Rapid tests (RTs), also known as point-of-care tests, usually release results within 30 min utes with no need for a qualified staff, equipment, or laboratory structure. The Brazilian Ministry of Health published a resolution in 2013, recommending the use of RTs for the diagnosis of HIV infection, where one positive RT must be followed by another different RT. This was meant to increase the chance of proper diagnosis in specific settings and special populations. However, data comparing and validating the different HIV RTs available in Brazil are scarce. Therefore, the present study seeks to evaluate eight anti-HIV RTs available in the Brazilian market regarding their analytical performance: sensitivity, specificity, positive and negative predictive values, positive and negative likelihood ratios, and accuracy. We also evaluated the agreement between kits (Kappa index) and the quality of the reading pat tern of the tests. This was an observational, analytical, and concordance study, in which pre viously defined positive and negative samples, based on their serological pattern for anti HIV antibodies (chemiluminescent immunoassay—ECLIA—used as screening and West ern Blot used as the confirmatory test) were tested. Analytical performance and Kappa index were calculated, considering a 95% CI and p<0.05. This study identified differences in the performances of the eight tested kits. Six out of eight RTs showed good performance and can be used in the routine laboratory and health care units as screening tests. Regard ing the quality of the RT band reading pattern, two brands had several samples showing quite faint bands, thus compromising its use in clinical and laboratory settings. |
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2023-06-12T20:13:01Z2023-06-12T20:13:01Z2020-08-131511110.1371/journal. pone.023743819326203http://hdl.handle.net/1843/54842Rapid tests (RTs), also known as point-of-care tests, usually release results within 30 min utes with no need for a qualified staff, equipment, or laboratory structure. The Brazilian Ministry of Health published a resolution in 2013, recommending the use of RTs for the diagnosis of HIV infection, where one positive RT must be followed by another different RT. This was meant to increase the chance of proper diagnosis in specific settings and special populations. However, data comparing and validating the different HIV RTs available in Brazil are scarce. Therefore, the present study seeks to evaluate eight anti-HIV RTs available in the Brazilian market regarding their analytical performance: sensitivity, specificity, positive and negative predictive values, positive and negative likelihood ratios, and accuracy. We also evaluated the agreement between kits (Kappa index) and the quality of the reading pat tern of the tests. This was an observational, analytical, and concordance study, in which pre viously defined positive and negative samples, based on their serological pattern for anti HIV antibodies (chemiluminescent immunoassay—ECLIA—used as screening and West ern Blot used as the confirmatory test) were tested. Analytical performance and Kappa index were calculated, considering a 95% CI and p<0.05. This study identified differences in the performances of the eight tested kits. Six out of eight RTs showed good performance and can be used in the routine laboratory and health care units as screening tests. Regard ing the quality of the RT band reading pattern, two brands had several samples showing quite faint bands, thus compromising its use in clinical and laboratory settings.engUniversidade Federal de Minas GeraisUFMGBrasilMED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTARPLOS ONEHIVSensibilidade e EspecificidadeSoropositividade para HIVUnidades de Diagnóstico RápidoHIVSensibilidade e Especificidadeteste laboratorial remotoTeste rápidoPerformance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0237438Feliciana Lage de Oliveira MarinhoNelson Luiz Linon Dos SantosSuzane Pretti Figueiredo NevesLeonardo de Souza Vasconcellosapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/54842/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALPerfomance evaluation of eight rapid tests to detect HIV infection A comparative study from Brazil pdfa.pdfPerfomance evaluation of eight rapid tests to detect HIV infection A comparative study from Brazil pdfa.pdfapplication/pdf377892https://repositorio.ufmg.br/bitstream/1843/54842/2/Perfomance%20evaluation%20of%20eight%20rapid%20tests%20to%20detect%20HIV%20infection%20A%20comparative%20study%20from%20Brazil%20pdfa.pdf62727885991fe0afbe9c6254f0b91a6eMD521843/548422023-06-12 18:24:57.192oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-06-12T21:24:57Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil |
title |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil |
spellingShingle |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil Feliciana Lage de Oliveira Marinho HIV Sensibilidade e Especificidade teste laboratorial remoto Teste rápido HIV Sensibilidade e Especificidade Soropositividade para HIV Unidades de Diagnóstico Rápido |
title_short |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil |
title_full |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil |
title_fullStr |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil |
title_full_unstemmed |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil |
title_sort |
Performance evaluation of eight rapid tests to detect hiv infection: a comparative study from Brazil |
author |
Feliciana Lage de Oliveira Marinho |
author_facet |
Feliciana Lage de Oliveira Marinho Nelson Luiz Linon Dos Santos Suzane Pretti Figueiredo Neves Leonardo de Souza Vasconcellos |
author_role |
author |
author2 |
Nelson Luiz Linon Dos Santos Suzane Pretti Figueiredo Neves Leonardo de Souza Vasconcellos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Feliciana Lage de Oliveira Marinho Nelson Luiz Linon Dos Santos Suzane Pretti Figueiredo Neves Leonardo de Souza Vasconcellos |
dc.subject.por.fl_str_mv |
HIV Sensibilidade e Especificidade teste laboratorial remoto Teste rápido |
topic |
HIV Sensibilidade e Especificidade teste laboratorial remoto Teste rápido HIV Sensibilidade e Especificidade Soropositividade para HIV Unidades de Diagnóstico Rápido |
dc.subject.other.pt_BR.fl_str_mv |
HIV Sensibilidade e Especificidade Soropositividade para HIV Unidades de Diagnóstico Rápido |
description |
Rapid tests (RTs), also known as point-of-care tests, usually release results within 30 min utes with no need for a qualified staff, equipment, or laboratory structure. The Brazilian Ministry of Health published a resolution in 2013, recommending the use of RTs for the diagnosis of HIV infection, where one positive RT must be followed by another different RT. This was meant to increase the chance of proper diagnosis in specific settings and special populations. However, data comparing and validating the different HIV RTs available in Brazil are scarce. Therefore, the present study seeks to evaluate eight anti-HIV RTs available in the Brazilian market regarding their analytical performance: sensitivity, specificity, positive and negative predictive values, positive and negative likelihood ratios, and accuracy. We also evaluated the agreement between kits (Kappa index) and the quality of the reading pat tern of the tests. This was an observational, analytical, and concordance study, in which pre viously defined positive and negative samples, based on their serological pattern for anti HIV antibodies (chemiluminescent immunoassay—ECLIA—used as screening and West ern Blot used as the confirmatory test) were tested. Analytical performance and Kappa index were calculated, considering a 95% CI and p<0.05. This study identified differences in the performances of the eight tested kits. Six out of eight RTs showed good performance and can be used in the routine laboratory and health care units as screening tests. Regard ing the quality of the RT band reading pattern, two brands had several samples showing quite faint bands, thus compromising its use in clinical and laboratory settings. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-08-13 |
dc.date.accessioned.fl_str_mv |
2023-06-12T20:13:01Z |
dc.date.available.fl_str_mv |
2023-06-12T20:13:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/54842 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal. pone.0237438 |
dc.identifier.issn.pt_BR.fl_str_mv |
19326203 |
identifier_str_mv |
10.1371/journal. pone.0237438 19326203 |
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http://hdl.handle.net/1843/54842 |
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eng |
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eng |
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PLOS ONE |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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MED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTAR |
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Universidade Federal de Minas Gerais |
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