Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1016/j.jhepr.2020.100117 http://hdl.handle.net/1843/53958 https://orcid.org/0000-0002-3137-991X https://orcid.org/0000-0003-4600-7593 https://orcid.org/0000-0002-4926-0618 https://orcid.org/0000-0002-9163-6279 https://orcid.org/0000-0002-0184-6433 https://orcid.org/0000-0003-4779-8972 https://orcid.org/0000-0001-8730-7336 https://orcid.org/0000-0001-7909-6970 https://orcid.org/0000-0001-5157-159X https://orcid.org/0000-0001-7724-890X https://orcid.org/0000-0003-2256-8652 https://orcid.org/0000-0001-8307-0810 https://orcid.org/0000-0003-3998-8012 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2023-05-25T20:03:31Z2023-05-25T20:03:31Z202024https://doi.org/10.1016/j.jhepr.2020.1001172589-5559http://hdl.handle.net/1843/53958https://orcid.org/0000-0002-3137-991Xhttps://orcid.org/0000-0003-4600-7593https://orcid.org/0000-0002-4926-0618https://orcid.org/0000-0002-9163-6279https://orcid.org/0000-0002-0184-6433https://orcid.org/0000-0003-4779-8972https://orcid.org/0000-0003-4779-8972https://orcid.org/0000-0001-8730-7336https://orcid.org/0000-0001-7909-6970https://orcid.org/0000-0001-5157-159Xhttps://orcid.org/0000-0001-7724-890Xhttps://orcid.org/0000-0003-2256-8652https://orcid.org/0000-0001-8307-0810https://orcid.org/0000-0003-3998-8012CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorBackground & Aims: The precise determination of non-alcoholic fatty liver disease (NAFLD) onset is challenging. Thus, the initial hepatic responses to fat accumulation, which may be fundamental to our understanding of NAFLD evolution and clinical outcomes, are largely unknown. Herein, we chronologically mapped the immunologic and metabolic changes in the liver during the early stages of fatty liver disease in mice and compared this with human NAFLD samples. Methods: Liver biopsies from patients with NAFLD (NAFLD activity score [NAS] 2–3) were collected for gene expression profiling. Mice received a high-fat diet for short periods to mimic initial steatosis and the hepatic immune response was investigated using a combination of confocal intravital imaging, gene expression, cell isolation, flow cytometry and bone marrow transplantation assays. Results: We observed major immunologic changes in patients with NAS 2–3 and in mice in the initial stages of NAFLD. In mice, these changes significantly increased mortality rates upon drug-induced liver injury, as well as predisposing mice to bacterial infections. Moreover, deletion of Toll-like receptor 4 in liver cells dampened tolerogenesis, particularly in Kupffer cells, in the initial stages of dietary insult. Conclusion: The hepatic immune system acts as a sentinel for early and minor changes in hepatic lipid content, mounting a biphasic response upon dietary insult. Priming of liver immune cells by gut-derived Toll-like receptor 4 ligands plays an important role in liver tolerance in initial phases, but continuous exposure to insults may lead to damage and reduced ability to control infections.engUniversidade Federal de Minas GeraisUFMGBrasilENF - DEPARTAMENTO DE NUTRIÇÃOICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICAICB - DEPARTAMENTO DE MORFOLOGIAMED - DEPARTAMENTO DE CIRURGIAMED - DEPARTAMENTO DE CLÍNICA MÉDICAJHEP ReportsHepatopatia gordurosa não alcoólicaFígadoSistema ImunitárioDietaMetabolismoImunidadeNAFLDLiverSteatosisImmune systemDietMetabolismIn vivo imagingImmunityImaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLDA imagem e a fenotipagem imunometabólica revelam alterações na resposta imune hepática nas fases iniciais da NAFLDinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S2589555920300513?via%3DihubAriane Barros DinizMaísa Mota AntunesViviane Aparecida de Souza LacerdaBrenda Naemi NakagakMaria Alice Freitas LopesHortência Maciel de Castro-OliveiraMatheus Silvério MattosKassiana Mafra BicalhoCamila Dutra Moreira de MirandaKaren Marques de Oliveira CostaMateus Eustáquio de Moura LopesDébora Moreira AlvarengaRaquel Carvalho-GontijoSarah Cozzer MarchesiDebora Romualdo LacerdaAlan Moreira de AraújoÉrika de CarvalhoBruna Araújo DavidMônica Morais SantosCristiano Xavier LimaJuliana Assis Silva GomesTereza Cristina Minto Fontes CalBruna Roque de SouzaCláudia Alves CoutoLuciana Costa FariaPaula Vieira Teixeira VidigalAdaliene Versiane Matos FerreiraSridhar RadhakrishnnanMatthew RicciAndré Gustavo OliveiraRafael Machado RezendeGustavo Batista Menezesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/53958/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALImaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD.pdfImaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD.pdfapplication/pdf4670795https://repositorio.ufmg.br/bitstream/1843/53958/2/Imaging%20and%20immunometabolic%20phenotyping%20uncover%20changes%20in%20the%20hepatic%20immune%20response%20in%20the%20early%20phases%20of%20NAFLD.pdf3d17576532dbc71bd968c0e015bbf59bMD521843/539582023-05-25 17:03:31.66oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-05-25T20:03:31Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD |
| dc.title.alternative.pt_BR.fl_str_mv |
A imagem e a fenotipagem imunometabólica revelam alterações na resposta imune hepática nas fases iniciais da NAFLD |
| title |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD |
| spellingShingle |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD Ariane Barros Diniz NAFLD Liver Steatosis Immune system Diet Metabolism In vivo imaging Immunity Hepatopatia gordurosa não alcoólica Fígado Sistema Imunitário Dieta Metabolismo Imunidade |
| title_short |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD |
| title_full |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD |
| title_fullStr |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD |
| title_full_unstemmed |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD |
| title_sort |
Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD |
| author |
Ariane Barros Diniz |
| author_facet |
Ariane Barros Diniz Maísa Mota Antunes Viviane Aparecida de Souza Lacerda Brenda Naemi Nakagak Maria Alice Freitas Lopes Hortência Maciel de Castro-Oliveira Matheus Silvério Mattos Kassiana Mafra Bicalho Camila Dutra Moreira de Miranda Karen Marques de Oliveira Costa Mateus Eustáquio de Moura Lopes Débora Moreira Alvarenga Raquel Carvalho-Gontijo Sarah Cozzer Marchesi Debora Romualdo Lacerda Alan Moreira de Araújo Érika de Carvalho Bruna Araújo David Mônica Morais Santos Cristiano Xavier Lima Juliana Assis Silva Gomes Tereza Cristina Minto Fontes Cal Bruna Roque de Souza Cláudia Alves Couto Luciana Costa Faria Paula Vieira Teixeira Vidigal Adaliene Versiane Matos Ferreira Sridhar Radhakrishnnan Matthew Ricci André Gustavo Oliveira Rafael Machado Rezende Gustavo Batista Menezes |
| author_role |
author |
| author2 |
Maísa Mota Antunes Viviane Aparecida de Souza Lacerda Brenda Naemi Nakagak Maria Alice Freitas Lopes Hortência Maciel de Castro-Oliveira Matheus Silvério Mattos Kassiana Mafra Bicalho Camila Dutra Moreira de Miranda Karen Marques de Oliveira Costa Mateus Eustáquio de Moura Lopes Débora Moreira Alvarenga Raquel Carvalho-Gontijo Sarah Cozzer Marchesi Debora Romualdo Lacerda Alan Moreira de Araújo Érika de Carvalho Bruna Araújo David Mônica Morais Santos Cristiano Xavier Lima Juliana Assis Silva Gomes Tereza Cristina Minto Fontes Cal Bruna Roque de Souza Cláudia Alves Couto Luciana Costa Faria Paula Vieira Teixeira Vidigal Adaliene Versiane Matos Ferreira Sridhar Radhakrishnnan Matthew Ricci André Gustavo Oliveira Rafael Machado Rezende Gustavo Batista Menezes |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ariane Barros Diniz Maísa Mota Antunes Viviane Aparecida de Souza Lacerda Brenda Naemi Nakagak Maria Alice Freitas Lopes Hortência Maciel de Castro-Oliveira Matheus Silvério Mattos Kassiana Mafra Bicalho Camila Dutra Moreira de Miranda Karen Marques de Oliveira Costa Mateus Eustáquio de Moura Lopes Débora Moreira Alvarenga Raquel Carvalho-Gontijo Sarah Cozzer Marchesi Debora Romualdo Lacerda Alan Moreira de Araújo Érika de Carvalho Bruna Araújo David Mônica Morais Santos Cristiano Xavier Lima Juliana Assis Silva Gomes Tereza Cristina Minto Fontes Cal Bruna Roque de Souza Cláudia Alves Couto Luciana Costa Faria Paula Vieira Teixeira Vidigal Adaliene Versiane Matos Ferreira Sridhar Radhakrishnnan Matthew Ricci André Gustavo Oliveira Rafael Machado Rezende Gustavo Batista Menezes |
| dc.subject.por.fl_str_mv |
NAFLD Liver Steatosis Immune system Diet Metabolism In vivo imaging Immunity |
| topic |
NAFLD Liver Steatosis Immune system Diet Metabolism In vivo imaging Immunity Hepatopatia gordurosa não alcoólica Fígado Sistema Imunitário Dieta Metabolismo Imunidade |
| dc.subject.other.pt_BR.fl_str_mv |
Hepatopatia gordurosa não alcoólica Fígado Sistema Imunitário Dieta Metabolismo Imunidade |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2020 |
| dc.date.issued.fl_str_mv |
2020 |
| dc.date.accessioned.fl_str_mv |
2023-05-25T20:03:31Z |
| dc.date.available.fl_str_mv |
2023-05-25T20:03:31Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/53958 |
| dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1016/j.jhepr.2020.100117 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2589-5559 |
| dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-3137-991X https://orcid.org/0000-0003-4600-7593 https://orcid.org/0000-0002-4926-0618 https://orcid.org/0000-0002-9163-6279 https://orcid.org/0000-0002-0184-6433 https://orcid.org/0000-0003-4779-8972 https://orcid.org/0000-0003-4779-8972 https://orcid.org/0000-0001-8730-7336 https://orcid.org/0000-0001-7909-6970 https://orcid.org/0000-0001-5157-159X https://orcid.org/0000-0001-7724-890X https://orcid.org/0000-0003-2256-8652 https://orcid.org/0000-0001-8307-0810 https://orcid.org/0000-0003-3998-8012 |
| url |
https://doi.org/10.1016/j.jhepr.2020.100117 http://hdl.handle.net/1843/53958 https://orcid.org/0000-0002-3137-991X https://orcid.org/0000-0003-4600-7593 https://orcid.org/0000-0002-4926-0618 https://orcid.org/0000-0002-9163-6279 https://orcid.org/0000-0002-0184-6433 https://orcid.org/0000-0003-4779-8972 https://orcid.org/0000-0001-8730-7336 https://orcid.org/0000-0001-7909-6970 https://orcid.org/0000-0001-5157-159X https://orcid.org/0000-0001-7724-890X https://orcid.org/0000-0003-2256-8652 https://orcid.org/0000-0001-8307-0810 https://orcid.org/0000-0003-3998-8012 |
| identifier_str_mv |
2589-5559 |
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eng |
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eng |
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JHEP Reports |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ENF - DEPARTAMENTO DE NUTRIÇÃO ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICB - DEPARTAMENTO DE MORFOLOGIA MED - DEPARTAMENTO DE CIRURGIA MED - DEPARTAMENTO DE CLÍNICA MÉDICA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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