Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-09598-8 http://hdl.handle.net/1843/68769 https://orcid.org/0000-0002-0210-8352 https://orcid.org/0000-0002-3758-4148 https://orcid.org/0000-0003-0161-5800 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2024-05-29T18:15:43Z2024-05-29T18:15:43Z202212http://dx.doi.org/10.1038/s41598-022-09598-82045-2322http://hdl.handle.net/1843/68769https://orcid.org/0000-0002-0210-8352https://orcid.org/0000-0002-3758-4148https://orcid.org/0000-0003-0161-5800CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPESP - Fundação de Amparo à Pesquisa do Estado de São PauloThe global spread of critical-priority antimicrobial-resistant Enterobacterales by food is a public health problem. Wild-caught seafood are broadly consumed worldwide, but exposure to land-based pollution can favor their contamination by clinically relevant antimicrobial-resistant bacteria. As part of the Grand Challenges Explorations: New Approaches to Characterize the Global Burden of Antimicrobial Resistance Program, we performed genomic surveillance and cell culture-based virulence investigation of WHO critical priority Enterobacterales isolated from marine bivalves collected in the Atlantic Coast of South America. Broad-spectrum cephalosporin-resistant Klebsiella pneumoniae and Escherichia coli isolates were recovered from eight distinct geographical locations. These strains harbored blaCTX-M-type or blaCMY-type genes. Most of the surveyed genomes confirmed the convergence of wide virulome and resistome (i.e., antimicrobials, heavy metals, biocides, and pesticides resistance). We identified strains belonging to the international high-risk clones K. pneumoniae ST307 and E. coli ST131 carrying important virulence genes, whereas in vitro experiments confirmed the high virulence potential of these strains. Thermolabile and thermostable toxins were identified in some strains, and all of them were biofilm producers. These data point to an alarming presence of resistance and virulence genes in marine environments, which may favor horizontal gene transfer and the spread of these traits to other bacterial species.engUniversidade Federal de Minas GeraisUFMGBrasilVET - DEPARTAMENTO DE CLÍNICA E CIRURGIAScientific ReportsEnterobacteriaceaeOrganização Mundial da SaúdeBivalvesIntoxicação por frutos do marEnterobacteralesWHOMarine bivalvesSeafoodConvergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalvesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.nature.com/articles/s41598-022-09598-8Vanessa BuerisFábio P. SelleraBruna FugaElder SanoMarcelo Pires Nogueira de CarvalhoSamuel Campanelli Freitas CoutoQuezia MouraNilton Lincopaninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/68769/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALConvergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves.pdfConvergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves.pdfapplication/pdf1500590https://repositorio.ufmg.br/bitstream/1843/68769/2/Convergence%20of%20virulence%20and%20resistance%20in%20international%20clones%20of%20WHO%20critical%20priority%20enterobacterales%20isolated%20from%20Marine%20Bivalves.pdf9500dba164cc21ee108ba502d17dcd40MD521843/687692024-05-29 15:15:44.557oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2024-05-29T18:15:44Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
title |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
spellingShingle |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves Vanessa Bueris Enterobacterales WHO Marine bivalves Seafood Enterobacteriaceae Organização Mundial da Saúde Bivalves Intoxicação por frutos do mar |
title_short |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
title_full |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
title_fullStr |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
title_full_unstemmed |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
title_sort |
Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
author |
Vanessa Bueris |
author_facet |
Vanessa Bueris Fábio P. Sellera Bruna Fuga Elder Sano Marcelo Pires Nogueira de Carvalho Samuel Campanelli Freitas Couto Quezia Moura Nilton Lincopan |
author_role |
author |
author2 |
Fábio P. Sellera Bruna Fuga Elder Sano Marcelo Pires Nogueira de Carvalho Samuel Campanelli Freitas Couto Quezia Moura Nilton Lincopan |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Vanessa Bueris Fábio P. Sellera Bruna Fuga Elder Sano Marcelo Pires Nogueira de Carvalho Samuel Campanelli Freitas Couto Quezia Moura Nilton Lincopan |
dc.subject.por.fl_str_mv |
Enterobacterales WHO Marine bivalves Seafood |
topic |
Enterobacterales WHO Marine bivalves Seafood Enterobacteriaceae Organização Mundial da Saúde Bivalves Intoxicação por frutos do mar |
dc.subject.other.pt_BR.fl_str_mv |
Enterobacteriaceae Organização Mundial da Saúde Bivalves Intoxicação por frutos do mar |
description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2024-05-29T18:15:43Z |
dc.date.available.fl_str_mv |
2024-05-29T18:15:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/68769 |
dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-09598-8 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-0210-8352 https://orcid.org/0000-0002-3758-4148 https://orcid.org/0000-0003-0161-5800 |
url |
http://dx.doi.org/10.1038/s41598-022-09598-8 http://hdl.handle.net/1843/68769 https://orcid.org/0000-0002-0210-8352 https://orcid.org/0000-0002-3758-4148 https://orcid.org/0000-0003-0161-5800 |
identifier_str_mv |
2045-2322 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
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https://repositorio.ufmg.br/bitstream/1843/68769/1/License.txt https://repositorio.ufmg.br/bitstream/1843/68769/2/Convergence%20of%20virulence%20and%20resistance%20in%20international%20clones%20of%20WHO%20critical%20priority%20enterobacterales%20isolated%20from%20Marine%20Bivalves.pdf |
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Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
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