Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma

Detalhes bibliográficos
Autor(a) principal: Eliane Macedo Sobrinho Santos
Data de Publicação: 2017
Outros Autores: Talita Antunes Guimarães, Hércules Otacílio Santos, Lilian Mendes Borborema Cangussu, Sabrina Ferreira de Jesus, Carlos Alberto de Carvalho Fraga, Cláudio Marcelo Cardoso, Sérgio Henrique Sousa Santos, Alfredo Maurício Batista de Paula, Ricardo Santiago Gomez, André Luiz Sena Guimarães, Lucyana Conceição Farias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1177/101042831769913
http://hdl.handle.net/1843/61670
Resumo: Leptin, one of the main hormones controlling energy homeostasis, has been associated with different cancer types. In oral cancer, its effect is not well understood. We investigated, through in vitro and in vivo assays, whether leptin can affect the neoplastic behavior of oral squamous cell carcinoma. Expression of genes possibly linked to the leptin pathway was assessed in leptin-treated oral squamous cell carcinoma cells and also in tissue samples of oral squamous cell carcinoma and oral mucosa, including leptin, leptin receptor, hypoxia-inducible factor 1-alpha, E-cadherin, matrix metalloproteinase-2, matrix metalloproteinase-9, Col1A1, Ki67, and mir-210. Leptin treatment favored higher rates of cell proliferation and migration, and reduced apoptosis. Accordingly, leptin-treated oral squamous cell carcinoma cells show decreased messenger RNA caspase-3 expression, and increased levels of E-cadherin, Col1A1, matrix metalloproteinase-2, matrix metalloproteinase-9, and mir-210. In tissue samples, hypoxia-inducible factor 1-alpha messenger RNA and protein expression of leptin and leptin receptor were high in oral squamous cell carcinoma cases. Serum leptin levels were increased in first clinical stages of the disease. In animal model, oral squamous cell carcinoma–induced mice show higher leptin receptor expression, and serum leptin level was increased in dysplasia group. Our findings suggest that leptin seems to exert an effect on oral squamous cell carcinoma cells behavior and also on molecular markers related to cell proliferation, migration, and tumor angiogenesis.
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spelling Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinomacarcinoma de células escamosas de bocacâncer de cabeça e pescoçoBIOLOGIA MOLECULARPATOGENIACarcinoma de células escamosasApoptoseCabeça - CâncerPescoço - CâncerBoca - CâncerNeovascularizaçãoLeptin, one of the main hormones controlling energy homeostasis, has been associated with different cancer types. In oral cancer, its effect is not well understood. We investigated, through in vitro and in vivo assays, whether leptin can affect the neoplastic behavior of oral squamous cell carcinoma. Expression of genes possibly linked to the leptin pathway was assessed in leptin-treated oral squamous cell carcinoma cells and also in tissue samples of oral squamous cell carcinoma and oral mucosa, including leptin, leptin receptor, hypoxia-inducible factor 1-alpha, E-cadherin, matrix metalloproteinase-2, matrix metalloproteinase-9, Col1A1, Ki67, and mir-210. Leptin treatment favored higher rates of cell proliferation and migration, and reduced apoptosis. Accordingly, leptin-treated oral squamous cell carcinoma cells show decreased messenger RNA caspase-3 expression, and increased levels of E-cadherin, Col1A1, matrix metalloproteinase-2, matrix metalloproteinase-9, and mir-210. In tissue samples, hypoxia-inducible factor 1-alpha messenger RNA and protein expression of leptin and leptin receptor were high in oral squamous cell carcinoma cases. Serum leptin levels were increased in first clinical stages of the disease. In animal model, oral squamous cell carcinoma–induced mice show higher leptin receptor expression, and serum leptin level was increased in dysplasia group. Our findings suggest that leptin seems to exert an effect on oral squamous cell carcinoma cells behavior and also on molecular markers related to cell proliferation, migration, and tumor angiogenesis.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal de Minas GeraisBrasilFAO - DEPARTAMENTO DE CLÍNICAICA - INSTITUTO DE CIÊNCIAS AGRÁRIASUFMG2023-12-04T13:15:29Z2023-12-04T13:15:29Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1177/1010428317699131423-0380http://hdl.handle.net/1843/61670engTumor BiologyEliane Macedo Sobrinho SantosTalita Antunes GuimarãesHércules Otacílio SantosLilian Mendes Borborema CangussuSabrina Ferreira de JesusCarlos Alberto de Carvalho FragaCláudio Marcelo CardosoSérgio Henrique Sousa SantosAlfredo Maurício Batista de PaulaRicardo Santiago GomezAndré Luiz Sena GuimarãesLucyana Conceição Fariasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2023-12-04T21:03:58Zoai:repositorio.ufmg.br:1843/61670Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2023-12-04T21:03:58Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
title Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
spellingShingle Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
Eliane Macedo Sobrinho Santos
carcinoma de células escamosas de boca
câncer de cabeça e pescoço
BIOLOGIA MOLECULAR
PATOGENIA
Carcinoma de células escamosas
Apoptose
Cabeça - Câncer
Pescoço - Câncer
Boca - Câncer
Neovascularização
title_short Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
title_full Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
title_fullStr Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
title_full_unstemmed Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
title_sort Leptin acts on neoplastic behavior and expression levels of genes related to hypoxia, angiogenesis, and invasiveness in oral squamous cell carcinoma
author Eliane Macedo Sobrinho Santos
author_facet Eliane Macedo Sobrinho Santos
Talita Antunes Guimarães
Hércules Otacílio Santos
Lilian Mendes Borborema Cangussu
Sabrina Ferreira de Jesus
Carlos Alberto de Carvalho Fraga
Cláudio Marcelo Cardoso
Sérgio Henrique Sousa Santos
Alfredo Maurício Batista de Paula
Ricardo Santiago Gomez
André Luiz Sena Guimarães
Lucyana Conceição Farias
author_role author
author2 Talita Antunes Guimarães
Hércules Otacílio Santos
Lilian Mendes Borborema Cangussu
Sabrina Ferreira de Jesus
Carlos Alberto de Carvalho Fraga
Cláudio Marcelo Cardoso
Sérgio Henrique Sousa Santos
Alfredo Maurício Batista de Paula
Ricardo Santiago Gomez
André Luiz Sena Guimarães
Lucyana Conceição Farias
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Eliane Macedo Sobrinho Santos
Talita Antunes Guimarães
Hércules Otacílio Santos
Lilian Mendes Borborema Cangussu
Sabrina Ferreira de Jesus
Carlos Alberto de Carvalho Fraga
Cláudio Marcelo Cardoso
Sérgio Henrique Sousa Santos
Alfredo Maurício Batista de Paula
Ricardo Santiago Gomez
André Luiz Sena Guimarães
Lucyana Conceição Farias
dc.subject.por.fl_str_mv carcinoma de células escamosas de boca
câncer de cabeça e pescoço
BIOLOGIA MOLECULAR
PATOGENIA
Carcinoma de células escamosas
Apoptose
Cabeça - Câncer
Pescoço - Câncer
Boca - Câncer
Neovascularização
topic carcinoma de células escamosas de boca
câncer de cabeça e pescoço
BIOLOGIA MOLECULAR
PATOGENIA
Carcinoma de células escamosas
Apoptose
Cabeça - Câncer
Pescoço - Câncer
Boca - Câncer
Neovascularização
description Leptin, one of the main hormones controlling energy homeostasis, has been associated with different cancer types. In oral cancer, its effect is not well understood. We investigated, through in vitro and in vivo assays, whether leptin can affect the neoplastic behavior of oral squamous cell carcinoma. Expression of genes possibly linked to the leptin pathway was assessed in leptin-treated oral squamous cell carcinoma cells and also in tissue samples of oral squamous cell carcinoma and oral mucosa, including leptin, leptin receptor, hypoxia-inducible factor 1-alpha, E-cadherin, matrix metalloproteinase-2, matrix metalloproteinase-9, Col1A1, Ki67, and mir-210. Leptin treatment favored higher rates of cell proliferation and migration, and reduced apoptosis. Accordingly, leptin-treated oral squamous cell carcinoma cells show decreased messenger RNA caspase-3 expression, and increased levels of E-cadherin, Col1A1, matrix metalloproteinase-2, matrix metalloproteinase-9, and mir-210. In tissue samples, hypoxia-inducible factor 1-alpha messenger RNA and protein expression of leptin and leptin receptor were high in oral squamous cell carcinoma cases. Serum leptin levels were increased in first clinical stages of the disease. In animal model, oral squamous cell carcinoma–induced mice show higher leptin receptor expression, and serum leptin level was increased in dysplasia group. Our findings suggest that leptin seems to exert an effect on oral squamous cell carcinoma cells behavior and also on molecular markers related to cell proliferation, migration, and tumor angiogenesis.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
2023-12-04T13:15:29Z
2023-12-04T13:15:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1177/101042831769913
1423-0380
http://hdl.handle.net/1843/61670
url https://doi.org/10.1177/101042831769913
http://hdl.handle.net/1843/61670
identifier_str_mv 1423-0380
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tumor Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
FAO - DEPARTAMENTO DE CLÍNICA
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
FAO - DEPARTAMENTO DE CLÍNICA
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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