The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats

Detalhes bibliográficos
Autor(a) principal: Bussuan, Luiz Antonio Maksoud
Data de Publicação: 2010
Outros Autores: Fagundes, Djalma José, Marks, Guido, Bussuan, Priscila Maksoud, Teruya, Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMS
DOI: http://dx.doi.org/10.1590/S0102-86502010000600008
Texto Completo: https://repositorio.ufms.br/handle/123456789/498
http://dx.doi.org/10.1590/S0102-86502010000600008
Resumo: PURPOSE: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. METHODS: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. The expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean ± sd (Shapiro-Wilks test and t Student test) (p < 0.05). RESULTS: In the animals of CG there was no significant difference between the FasL expression of the top (10.75±3.33) and basal (11.14±3.53) colon crypt (p=0.34293740). In the animals of AOM there was no significant difference between the FasL expression of the top (8.86±4.19) and basal (8.99±4.08) colon crypt (p=0.78486003). In the animals of CG (10.95±3.43) and AOM (8.92±4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75±3.33) and AOM (8.86±4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14±3.53) and AOM (8.99±4.08) in the basal colon crypt (p=0.00000381**). CONCLUSION: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.
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spelling 2011-09-14T13:47:34Z2021-09-30T19:56:14Z2010BUSSUAN, Luiz Antonio Maksoud et al . The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats. Acta Cir. Bras., São Paulo, v. 25, n. 6, dez. 2010 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000600008&lng=pt&nrm=iso>. acessos em 14 set. 2011. http://dx.doi.org/10.1590/S0102-86502010000600008.https://repositorio.ufms.br/handle/123456789/498http://dx.doi.org/10.1590/S0102-86502010000600008PURPOSE: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. METHODS: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. The expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean ± sd (Shapiro-Wilks test and t Student test) (p < 0.05). RESULTS: In the animals of CG there was no significant difference between the FasL expression of the top (10.75±3.33) and basal (11.14±3.53) colon crypt (p=0.34293740). In the animals of AOM there was no significant difference between the FasL expression of the top (8.86±4.19) and basal (8.99±4.08) colon crypt (p=0.78486003). In the animals of CG (10.95±3.43) and AOM (8.92±4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75±3.33) and AOM (8.86±4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14±3.53) and AOM (8.99±4.08) in the basal colon crypt (p=0.00000381**). CONCLUSION: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.OBJETIVO: Avaliar o marcador de apoptose Fas ligante (FasL) em um modelo de estresse oxidativo induzido pelo azoximetano (AOM) na cripta de cólon em ratos. MÉTODOS: 14 ratos Wistar foram distribuídos em dois grupos: controle (n=7) e AOM (n=7). A AOM (5mg/kg) ou solução salina foi aplicada via subcutânea e a coleta de amostras de colo proximal ocorreu 3 horas após. A FasL foi quantificada pelo percentual de áreas no topo, base e toda a cripta. Os resultados foram submetidos aos testes de Shapiro-Wilks e t de Student (p < 0,05). RESULTADOS: No grupo GC, não houve diferença significativa entre a expressão da FasL no topo (10,75 ± 3,33) e base (11,14 ± 3,53) da cripta (p=0,34293740). No grupo AOM não houve diferença significativa entre a expressão da FasL no topo (8,86 ± 4,19) e base (8,99 ± 4,08) e cripta (p=0,78486003). No grupo GC (10,95 ± 3,43) e AOM (8,92 ± 4,13), houve uma diferença significativa da expressão da FasL (p=0,026466821). Redução significativa na expressão da FasL ocorreu nos em GC (10,75 ± 3,33) e AOM (8,86 ± 4,19) no topo da cripta (p = 0,00003755*). Foi observada diminuição significativa em GC (11,14 ± 3,53) e AOM (8,99 ± 4,08) na base da cripta (p=0,00000381**). CONCLUSÃO: Azoximetano induziu o estresse oxidativo identificado pela diminuição significativa da expressão da FasL, embora não haja diferença significativa entre a base e parte superior da cripta associada à ativação de consumo do FasL.engActa Cirurgica BrasileiraProteína Ligante FasAzoximetanoEstresse OxidativoApoptoseRatosFas Ligand ProteinAzoxymethaneOxidative StressApoptosisRatsThe role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of ratsO papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBussuan, Luiz Antonio MaksoudFagundes, Djalma JoséMarks, GuidoBussuan, Priscila MaksoudTeruya, Robertoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILThe role of Fas ligand protein.pdf.jpgThe role of Fas ligand protein.pdf.jpgGenerated Thumbnailimage/jpeg1547https://repositorio.ufms.br/bitstream/123456789/498/4/The%20role%20of%20Fas%20ligand%20protein.pdf.jpgc64fa4c7e5ad58e39800a5327fdae5e2MD54ORIGINALThe role of Fas ligand protein.pdfThe role of Fas ligand protein.pdfapplication/pdf308299https://repositorio.ufms.br/bitstream/123456789/498/1/The%20role%20of%20Fas%20ligand%20protein.pdff3086d284b95f0dc6958bbeeedd183efMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufms.br/bitstream/123456789/498/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTThe role of Fas ligand protein.pdf.txtThe role of Fas ligand protein.pdf.txtExtracted texttext/plain27334https://repositorio.ufms.br/bitstream/123456789/498/3/The%20role%20of%20Fas%20ligand%20protein.pdf.txtbe64c5bb32c31a50ba910e0af7e76f64MD53123456789/4982021-09-30 15:56:14.624oai:repositorio.ufms.br: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Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242021-09-30T19:56:14Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false
dc.title.pt_BR.fl_str_mv The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
dc.title.alternative.pt_BR.fl_str_mv O papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratos
title The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
spellingShingle The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
Bussuan, Luiz Antonio Maksoud
Proteína Ligante Fas
Azoximetano
Estresse Oxidativo
Apoptose
Ratos
Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
title_short The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_full The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_fullStr The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_full_unstemmed The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_sort The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
author Bussuan, Luiz Antonio Maksoud
author_facet Bussuan, Luiz Antonio Maksoud
Fagundes, Djalma José
Marks, Guido
Bussuan, Priscila Maksoud
Teruya, Roberto
author_role author
author2 Fagundes, Djalma José
Marks, Guido
Bussuan, Priscila Maksoud
Teruya, Roberto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bussuan, Luiz Antonio Maksoud
Fagundes, Djalma José
Marks, Guido
Bussuan, Priscila Maksoud
Teruya, Roberto
dc.subject.por.fl_str_mv Proteína Ligante Fas
Azoximetano
Estresse Oxidativo
Apoptose
Ratos
Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
topic Proteína Ligante Fas
Azoximetano
Estresse Oxidativo
Apoptose
Ratos
Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
description PURPOSE: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. METHODS: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. The expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean ± sd (Shapiro-Wilks test and t Student test) (p < 0.05). RESULTS: In the animals of CG there was no significant difference between the FasL expression of the top (10.75±3.33) and basal (11.14±3.53) colon crypt (p=0.34293740). In the animals of AOM there was no significant difference between the FasL expression of the top (8.86±4.19) and basal (8.99±4.08) colon crypt (p=0.78486003). In the animals of CG (10.95±3.43) and AOM (8.92±4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75±3.33) and AOM (8.86±4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14±3.53) and AOM (8.99±4.08) in the basal colon crypt (p=0.00000381**). CONCLUSION: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2011-09-14T13:47:34Z
dc.date.available.fl_str_mv 2021-09-30T19:56:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BUSSUAN, Luiz Antonio Maksoud et al . The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats. Acta Cir. Bras., São Paulo, v. 25, n. 6, dez. 2010 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000600008&lng=pt&nrm=iso>. acessos em 14 set. 2011. http://dx.doi.org/10.1590/S0102-86502010000600008.
dc.identifier.uri.fl_str_mv https://repositorio.ufms.br/handle/123456789/498
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1590/S0102-86502010000600008
identifier_str_mv BUSSUAN, Luiz Antonio Maksoud et al . The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats. Acta Cir. Bras., São Paulo, v. 25, n. 6, dez. 2010 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000600008&lng=pt&nrm=iso>. acessos em 14 set. 2011. http://dx.doi.org/10.1590/S0102-86502010000600008.
url https://repositorio.ufms.br/handle/123456789/498
http://dx.doi.org/10.1590/S0102-86502010000600008
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publisher.none.fl_str_mv Acta Cirurgica Brasileira
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instname:Universidade Federal de Mato Grosso do Sul (UFMS)
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