The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats

Detalhes bibliográficos
Autor(a) principal: Bussuan, Luiz Antonio Maksoud [UNIFESP]
Data de Publicação: 2010
Outros Autores: Fagundes, Djalma Jose [UNIFESP], Marks, Guido, Bussuan, Priscila Maksoud, Teruya, Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502010000600008
http://repositorio.unifesp.br/handle/11600/33018
Resumo: Purpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.
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spelling The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of ratsO papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratosFas Ligand ProteinAzoxymethaneOxidative StressApoptosisRatsPurpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.UNIFESP SP, Dept Surg, Postgrad Program Surg & Experimentat, Div Operat Tech & Expt Surg, São Paulo, BrazilUFMS, Dept Surg, Campo Grande, MS USAMoreira Salles Inst IMS, Rio de Janeiro, BrazilFed Univ Mato Grosso Sul UFMS, Expt Carcinogenesis Lab, Cent Anim Facil, Campo Grande, MS USAFed Univ São Paulo UNIFESP, Surg & Experimentat Postgrad Program, São Paulo, BrazilUNIFESP SP, Dept Surg, Postgrad Program Surg & Experimentat, Div Operat Tech & Expt Surg, São Paulo, BrazilFed Univ São Paulo UNIFESP, Surg & Experimentat Postgrad Program, São Paulo, BrazilWeb of ScienceActa Cirurgica BrasileiraUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de Mato Grosso do Sul (UFMS)Moreira Salles Inst IMSBussuan, Luiz Antonio Maksoud [UNIFESP]Fagundes, Djalma Jose [UNIFESP]Marks, GuidoBussuan, Priscila MaksoudTeruya, Roberto2016-01-24T14:05:37Z2016-01-24T14:05:37Z2010-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion501-506application/pdfhttp://dx.doi.org/10.1590/S0102-86502010000600008Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010.10.1590/S0102-865020100006000080102-8650S0102-86502010000600008http://repositorio.unifesp.br/handle/11600/33018WOS:000284779000008engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T12:31:56Zoai:repositorio.unifesp.br/:11600/33018Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T12:31:56Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
O papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratos
title The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
spellingShingle The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
Bussuan, Luiz Antonio Maksoud [UNIFESP]
Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
title_short The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_full The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_fullStr The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_full_unstemmed The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
title_sort The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
author Bussuan, Luiz Antonio Maksoud [UNIFESP]
author_facet Bussuan, Luiz Antonio Maksoud [UNIFESP]
Fagundes, Djalma Jose [UNIFESP]
Marks, Guido
Bussuan, Priscila Maksoud
Teruya, Roberto
author_role author
author2 Fagundes, Djalma Jose [UNIFESP]
Marks, Guido
Bussuan, Priscila Maksoud
Teruya, Roberto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Mato Grosso do Sul (UFMS)
Moreira Salles Inst IMS
dc.contributor.author.fl_str_mv Bussuan, Luiz Antonio Maksoud [UNIFESP]
Fagundes, Djalma Jose [UNIFESP]
Marks, Guido
Bussuan, Priscila Maksoud
Teruya, Roberto
dc.subject.por.fl_str_mv Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
topic Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
description Purpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.
publishDate 2010
dc.date.none.fl_str_mv 2010-11-01
2016-01-24T14:05:37Z
2016-01-24T14:05:37Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502010000600008
Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010.
10.1590/S0102-86502010000600008
0102-8650
S0102-86502010000600008
http://repositorio.unifesp.br/handle/11600/33018
WOS:000284779000008
url http://dx.doi.org/10.1590/S0102-86502010000600008
http://repositorio.unifesp.br/handle/11600/33018
identifier_str_mv Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010.
10.1590/S0102-86502010000600008
0102-8650
S0102-86502010000600008
WOS:000284779000008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 501-506
application/pdf
dc.publisher.none.fl_str_mv Acta Cirurgica Brasileira
publisher.none.fl_str_mv Acta Cirurgica Brasileira
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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