Efeito da peçonha de escorpiões do gênero Tityus sobre a atividade das enzimas do sistema purinérgico em linfócitos e plaquetas de sangue periférico humano.

• Autor(a) principal: TELMA RODRIGUES DA SILVA
• Data de Publicação: 2022
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFMS
• Texto Completo: https://repositorio.ufms.br/handle/123456789/4738
• topic: Investigação do efeito da peçonha de Tityus serrulatus e Tityus paraguayensis sobre as atividades das enzimas do sistema purinérgico em plaquetas e linfócitos humanos.

The purinergic system is a signalling system, where the purine nucleotides, ATP (Adenosine 5′-triphosphate) and ADP (Adenosine diphosphate), and the nucleoside, adenosine, act as extracellular messengers. CD39 (ecto-nucleoside triphosphate diphosphohydrolase 1, E-NTPDase1) converts ATP or ADP into AMP, and then CD73 (ecto-5'- nucleotidase, Ecto5'NTase) dephosphorylates AMP into adenosine. CD39 and CD73 regulate the function of several immune cell types, including lymphocytes. Furthermore, there is evidence that changes in CD39 expression and activity affects the potential thrombogenic of a tissue. The objective of this study was to assay the enzymatic activity of CD39 and CD73 in the presence of scorpion venom from Tityus confluens (Tc) and Tityus paraguayensis (Tp) in human lymphocyte and platelet. Our results demonstrate that T.c venom has decreased CD39 activity in ATP hydrolysis in peripheral blood lymphocytes when compared to control (P<0.05). On the other hand, the results demonstrate no effect on CD39 and CD73 enzymes in peripheral blood platelets. This study shows the first time the effect of Tityus scorpion venom on CD39 and CD73 enzymes in peripheral blood cells. Others studies, with higher doses, can elucidate the main action pathway between venoms and the blood.