AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMS |
Texto Completo: | https://repositorio.ufms.br/handle/123456789/8566 |
Resumo: | Introduction. The nomenclature of the Candida genus was accepted as an official name only in 1954, to accommodate a group of yeasts that presented specific mycological characteristics. In the epidemiological context, they are opportunistic pathogens found in several environments, but mainly in the human body, which can cause since superficial until systemic infections. The present study aimed the Candda glabrata – the molecular identification of the isolates obtained from different clinical specimens of hospitalized patients, its distribution as to the hospital units, and the evaluation of the susceptibility/resistance to antifungal compounds belonged to different pharmacological groups. Patients and Methods. Isolates from several clinical specimens obtained from 80 patients admitted at different hospital units of a tertiary hospital were studied. The molecular identification was performed using the MALDI-TOF MS, and the evaluation of the susceptibility antifungal testing was carried out according to the EUCAST procedures for six compounds – one polienic (amphotericin B deoxycholate – AmB), two triazole compounds (fluconazole-FLC and vorionazole-VRC), and three equinocandins (caspofungin, micafungin and anidulafungin). Results. All the isolates were identified as Candida glabrata stricto sensu. These strains presented susceptibility to AmB, to the three equinocandins, and to VRC, but intermediate susceptibility to FLC. The prevalence of resistance to AmB and to VRC was higher than to the equinocandins. Although into the susceptibility range, the minimal inhibitory concentration of AmB, anidulafungin, and micafungin found in the present study was higher than those published by EUCAST, taken as a reference. Conclusions. The present study permits to conclude that the strain Candida glabrata stricto sensu predominates in the different clinical specimens isolated from patients of this region, and that they are not susceptible to fluconazole. For this reason, this triazole compound cannot be the first choice to treat severe patients from this region. The high prevalence of elevated minimal inhibitory concentrations, although into the susceptibility range, indicates the need not only the identification of the Candida spp. at the level of species, but also the evaluation of the susceptibility profile of all the Candida glabrata isolates, whose results must be send to the clinical staff. |
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2024-03-14T11:49:02Z2024-03-14T11:49:02Z2024https://repositorio.ufms.br/handle/123456789/8566Introduction. The nomenclature of the Candida genus was accepted as an official name only in 1954, to accommodate a group of yeasts that presented specific mycological characteristics. In the epidemiological context, they are opportunistic pathogens found in several environments, but mainly in the human body, which can cause since superficial until systemic infections. The present study aimed the Candda glabrata – the molecular identification of the isolates obtained from different clinical specimens of hospitalized patients, its distribution as to the hospital units, and the evaluation of the susceptibility/resistance to antifungal compounds belonged to different pharmacological groups. Patients and Methods. Isolates from several clinical specimens obtained from 80 patients admitted at different hospital units of a tertiary hospital were studied. The molecular identification was performed using the MALDI-TOF MS, and the evaluation of the susceptibility antifungal testing was carried out according to the EUCAST procedures for six compounds – one polienic (amphotericin B deoxycholate – AmB), two triazole compounds (fluconazole-FLC and vorionazole-VRC), and three equinocandins (caspofungin, micafungin and anidulafungin). Results. All the isolates were identified as Candida glabrata stricto sensu. These strains presented susceptibility to AmB, to the three equinocandins, and to VRC, but intermediate susceptibility to FLC. The prevalence of resistance to AmB and to VRC was higher than to the equinocandins. Although into the susceptibility range, the minimal inhibitory concentration of AmB, anidulafungin, and micafungin found in the present study was higher than those published by EUCAST, taken as a reference. Conclusions. The present study permits to conclude that the strain Candida glabrata stricto sensu predominates in the different clinical specimens isolated from patients of this region, and that they are not susceptible to fluconazole. For this reason, this triazole compound cannot be the first choice to treat severe patients from this region. The high prevalence of elevated minimal inhibitory concentrations, although into the susceptibility range, indicates the need not only the identification of the Candida spp. at the level of species, but also the evaluation of the susceptibility profile of all the Candida glabrata isolates, whose results must be send to the clinical staff.Introdução. A denominação de Candida, como gênero de leveduras que apresentam características micológicas específicas, só foi oficializada em 1954. No contexto epidemiológico, são patógenos oportunistas encontrados em diversos ambientes, principalmente no corpo humano, podendo causar desde infecções superficiais até sistêmicas. O presente estudo focalizou a espécie Candida glabrata, desde a identificação molecular de isolados obtidos de diferentes espécimes clínicos de pacientes hospitalizados, sua distribuição segundo as unidades hospitalares e a avaliação da sensibilidade / resistência a compostos antifúngicos pertencentes a diferentes grupos farmacológicos. Pacientes e Métodos. Foram estudados isolados de vários espécimes clínicos de 80 pacientes atendidos em diferentes unidades de um hospital terciário. A identificação molecular foi feita utilizando-se a técnica MALDITOF MS e a avaliação da sensibilidade a antifúngicos foi realizada usando-se a metodologia preconizada pelo EUCAST e seis antifúngicos – um poliênico (anfotericina B desoxicolato - AmB), dois derivados triazólicos (fluconazol-FLC e voriconazol-VRC) e três equinocandinas (caspofungina, micafungina e anidulafungina). Resultados. Todos os isolados foram identificados como Candida glabrata stricto sensu. As cepas apresentavam elevada sensibilidade à AmB, às três equinocandinas e ao VRC, mas sensibilidade intermediária ao FLC. A prevalência de resistência à AmB e ao VRC era maior que às equinocandinas. Embora ainda dentro das faixas de sensibilidade, as concentrações inibitórias mínimas de AmB, anidulafungina e micafungina do presente estudo eram maiores que as publicadas pelo EUCAST, tomadas como referência. Conclusões. O presente estudo permite concluir que as cepas de Candida glabrata stricto sensu predominam nos diferentes espécimes clínicos avaliados nesta região e que não são sensíveis ao fluconazol, motivo pelo qual este composto antifúngico não deve ser primeira escolha no tratamento de pacientes graves. A alta prevalência de concentrações inibitórias mínimas elevadas, embora ainda dentro da faixa de sensibilidade, indicam a necessidade de identificação de todas as amostras em nível de espécie e, para os isolados de C. glabrata, a realização rotineira do teste de sensibilidade antifúngica, cujos resultados devem ser divulgados ao corpo clínico.Fundação Universidade Federal de Mato Grosso do SulUFMSBrasilCandida glabrataMALDI-TOF MSCandidíases. Compostos antifúngicosTeste de sensibilidade antifúngica.AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIOinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisRinaldo Poncio MendesMaricelma Francelino Fialho Cândidoinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSORIGINALDISSERTAÇÃO MARICELMA FRANCELINO FIALHO CANDIDO.pdfDISSERTAÇÃO MARICELMA FRANCELINO FIALHO CANDIDO.pdfapplication/pdf1305371https://repositorio.ufms.br/bitstream/123456789/8566/-1/DISSERTA%c3%87%c3%83O%20MARICELMA%20FRANCELINO%20FIALHO%20CANDIDO.pdf62a9a3355bfb1c35f000499fb2ebfff9MD5-1123456789/85662024-03-14 07:49:03.203oai:repositorio.ufms.br:123456789/8566Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242024-03-14T11:49:03Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false |
dc.title.pt_BR.fl_str_mv |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO |
title |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO |
spellingShingle |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO Maricelma Francelino Fialho Cândido Candida glabrata MALDI-TOF MS Candidíases. Compostos antifúngicos Teste de sensibilidade antifúngica. |
title_short |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO |
title_full |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO |
title_fullStr |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO |
title_full_unstemmed |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO |
title_sort |
AVALIAÇÃO DE INFECÇÃO POR Candida glabrata EM HOSPITAL PÚBLICO TERCIÁRIO |
author |
Maricelma Francelino Fialho Cândido |
author_facet |
Maricelma Francelino Fialho Cândido |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rinaldo Poncio Mendes |
dc.contributor.author.fl_str_mv |
Maricelma Francelino Fialho Cândido |
contributor_str_mv |
Rinaldo Poncio Mendes |
dc.subject.por.fl_str_mv |
Candida glabrata MALDI-TOF MS Candidíases. Compostos antifúngicos Teste de sensibilidade antifúngica. |
topic |
Candida glabrata MALDI-TOF MS Candidíases. Compostos antifúngicos Teste de sensibilidade antifúngica. |
description |
Introduction. The nomenclature of the Candida genus was accepted as an official name only in 1954, to accommodate a group of yeasts that presented specific mycological characteristics. In the epidemiological context, they are opportunistic pathogens found in several environments, but mainly in the human body, which can cause since superficial until systemic infections. The present study aimed the Candda glabrata – the molecular identification of the isolates obtained from different clinical specimens of hospitalized patients, its distribution as to the hospital units, and the evaluation of the susceptibility/resistance to antifungal compounds belonged to different pharmacological groups. Patients and Methods. Isolates from several clinical specimens obtained from 80 patients admitted at different hospital units of a tertiary hospital were studied. The molecular identification was performed using the MALDI-TOF MS, and the evaluation of the susceptibility antifungal testing was carried out according to the EUCAST procedures for six compounds – one polienic (amphotericin B deoxycholate – AmB), two triazole compounds (fluconazole-FLC and vorionazole-VRC), and three equinocandins (caspofungin, micafungin and anidulafungin). Results. All the isolates were identified as Candida glabrata stricto sensu. These strains presented susceptibility to AmB, to the three equinocandins, and to VRC, but intermediate susceptibility to FLC. The prevalence of resistance to AmB and to VRC was higher than to the equinocandins. Although into the susceptibility range, the minimal inhibitory concentration of AmB, anidulafungin, and micafungin found in the present study was higher than those published by EUCAST, taken as a reference. Conclusions. The present study permits to conclude that the strain Candida glabrata stricto sensu predominates in the different clinical specimens isolated from patients of this region, and that they are not susceptible to fluconazole. For this reason, this triazole compound cannot be the first choice to treat severe patients from this region. The high prevalence of elevated minimal inhibitory concentrations, although into the susceptibility range, indicates the need not only the identification of the Candida spp. at the level of species, but also the evaluation of the susceptibility profile of all the Candida glabrata isolates, whose results must be send to the clinical staff. |
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2024 |
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2024-03-14T11:49:02Z |
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2024-03-14T11:49:02Z |
dc.date.issued.fl_str_mv |
2024 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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https://repositorio.ufms.br/handle/123456789/8566 |
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Fundação Universidade Federal de Mato Grosso do Sul |
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UFMS |
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Brasil |
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Fundação Universidade Federal de Mato Grosso do Sul |
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