Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil

Detalhes bibliográficos
Autor(a) principal: Araújo, Cristina Pires de
Data de Publicação: 2005
Outros Autores: Leite, Clarice Queico Fugimura, Prince, Karina Andrade de, Jorge, Klaudia dos Santos Gonçalves, Osório, Ana Luiza Alves Rosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMS
Texto Completo: https://repositorio.ufms.br/handle/123456789/1278
http://dx.doi.org/10.1590/S0074-02762005000700013.
Resumo: The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
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spelling 2012-04-19T13:13:57Z2021-09-30T19:56:39Z2005-11ARAUJO, Cristina Pires de et al . Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, Nov. 2005 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700013&lng=en&nrm=iso>. access on 19 Apr. 2012.https://repositorio.ufms.br/handle/123456789/1278http://dx.doi.org/10.1590/S0074-02762005000700013.The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.engMemórias do Instituto Oswaldo CruzMycobacterium BovisMicobactérias não TuberculosasReação em Cadeia da PolimeraseBovinosMatadourosNontuberculous MycobacteriaPolymerase Chain ReactionCattleAbattoirsMycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAraújo, Cristina Pires deLeite, Clarice Queico FugimuraPrince, Karina Andrade deJorge, Klaudia dos Santos GonçalvesOsório, Ana Luiza Alves Rosainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILMycobacterium bovis identification by a molecular.pdf.jpgMycobacterium bovis identification by a molecular.pdf.jpgGenerated Thumbnailimage/jpeg1699https://repositorio.ufms.br/bitstream/123456789/1278/4/Mycobacterium%20bovis%20identification%20by%20a%20molecular.pdf.jpg9d0ed185e695febc3805b1e964e2828aMD54ORIGINALMycobacterium bovis identification by a molecular.pdfMycobacterium bovis identification by a molecular.pdfapplication/pdf592978https://repositorio.ufms.br/bitstream/123456789/1278/1/Mycobacterium%20bovis%20identification%20by%20a%20molecular.pdfb218d4ce4f9773ae31667422408a25dfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufms.br/bitstream/123456789/1278/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTMycobacterium bovis identification by a molecular.pdf.txtMycobacterium bovis identification by a molecular.pdf.txtExtracted texttext/plain15308https://repositorio.ufms.br/bitstream/123456789/1278/3/Mycobacterium%20bovis%20identification%20by%20a%20molecular.pdf.txtb283ba4e81a075da9e840409b2ac11a5MD53123456789/12782021-09-30 15:56:39.946oai:repositorio.ufms.br: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Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242021-09-30T19:56:39Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false
dc.title.pt_BR.fl_str_mv Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
spellingShingle Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
Araújo, Cristina Pires de
Mycobacterium Bovis
Micobactérias não Tuberculosas
Reação em Cadeia da Polimerase
Bovinos
Matadouros
Nontuberculous Mycobacteria
Polymerase Chain Reaction
Cattle
Abattoirs
title_short Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_full Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_fullStr Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_full_unstemmed Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_sort Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
author Araújo, Cristina Pires de
author_facet Araújo, Cristina Pires de
Leite, Clarice Queico Fugimura
Prince, Karina Andrade de
Jorge, Klaudia dos Santos Gonçalves
Osório, Ana Luiza Alves Rosa
author_role author
author2 Leite, Clarice Queico Fugimura
Prince, Karina Andrade de
Jorge, Klaudia dos Santos Gonçalves
Osório, Ana Luiza Alves Rosa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Araújo, Cristina Pires de
Leite, Clarice Queico Fugimura
Prince, Karina Andrade de
Jorge, Klaudia dos Santos Gonçalves
Osório, Ana Luiza Alves Rosa
dc.subject.por.fl_str_mv Mycobacterium Bovis
Micobactérias não Tuberculosas
Reação em Cadeia da Polimerase
Bovinos
Matadouros
Nontuberculous Mycobacteria
Polymerase Chain Reaction
Cattle
Abattoirs
topic Mycobacterium Bovis
Micobactérias não Tuberculosas
Reação em Cadeia da Polimerase
Bovinos
Matadouros
Nontuberculous Mycobacteria
Polymerase Chain Reaction
Cattle
Abattoirs
description The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
publishDate 2005
dc.date.issued.fl_str_mv 2005-11
dc.date.accessioned.fl_str_mv 2012-04-19T13:13:57Z
dc.date.available.fl_str_mv 2021-09-30T19:56:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ARAUJO, Cristina Pires de et al . Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, Nov. 2005 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700013&lng=en&nrm=iso>. access on 19 Apr. 2012.
dc.identifier.uri.fl_str_mv https://repositorio.ufms.br/handle/123456789/1278
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1590/S0074-02762005000700013.
identifier_str_mv ARAUJO, Cristina Pires de et al . Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, Nov. 2005 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700013&lng=en&nrm=iso>. access on 19 Apr. 2012.
url https://repositorio.ufms.br/handle/123456789/1278
http://dx.doi.org/10.1590/S0074-02762005000700013.
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