Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil

Detalhes bibliográficos
Autor(a) principal: Araújo, Cristina Pires de [UNESP]
Data de Publicação: 2005
Outros Autores: Leite, Clarice Queico Fujimura [UNESP], Prince, Karina Andrade de [UNESP], Jorge, Klaudia dos Santos Gonçalves, Osório, Ana Luiza Alves Rosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762005000700013
http://hdl.handle.net/11449/8019
Resumo: The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
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spelling Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, BrazilMycobacterium bovisnontuberculous mycobacteriapolymerase chain reactionPCR- restriction enzyme pattern analysiscattlepost-mortem inspectionabattoirsThe presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências FarmacêuticasUniversidade Federal de Mato Grosso do SulUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências FarmacêuticasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Araújo, Cristina Pires de [UNESP]Leite, Clarice Queico Fujimura [UNESP]Prince, Karina Andrade de [UNESP]Jorge, Klaudia dos Santos GonçalvesOsório, Ana Luiza Alves Rosa2014-05-20T13:25:21Z2014-05-20T13:25:21Z2005-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article749-752application/pdfhttp://dx.doi.org/10.1590/S0074-02762005000700013Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 100, n. 7, p. 749-752, 2005.0074-0276http://hdl.handle.net/11449/801910.1590/S0074-02762005000700013S0074-02762005000700013S0074-02762005000700013.pdf2114570774349859SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:08:36Zoai:repositorio.unesp.br:11449/8019Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:59:59.289928Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
spellingShingle Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
Araújo, Cristina Pires de [UNESP]
Mycobacterium bovis
nontuberculous mycobacteria
polymerase chain reaction
PCR- restriction enzyme pattern analysis
cattle
post-mortem inspection
abattoirs
title_short Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_full Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_fullStr Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_full_unstemmed Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
title_sort Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
author Araújo, Cristina Pires de [UNESP]
author_facet Araújo, Cristina Pires de [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Prince, Karina Andrade de [UNESP]
Jorge, Klaudia dos Santos Gonçalves
Osório, Ana Luiza Alves Rosa
author_role author
author2 Leite, Clarice Queico Fujimura [UNESP]
Prince, Karina Andrade de [UNESP]
Jorge, Klaudia dos Santos Gonçalves
Osório, Ana Luiza Alves Rosa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.author.fl_str_mv Araújo, Cristina Pires de [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Prince, Karina Andrade de [UNESP]
Jorge, Klaudia dos Santos Gonçalves
Osório, Ana Luiza Alves Rosa
dc.subject.por.fl_str_mv Mycobacterium bovis
nontuberculous mycobacteria
polymerase chain reaction
PCR- restriction enzyme pattern analysis
cattle
post-mortem inspection
abattoirs
topic Mycobacterium bovis
nontuberculous mycobacteria
polymerase chain reaction
PCR- restriction enzyme pattern analysis
cattle
post-mortem inspection
abattoirs
description The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
publishDate 2005
dc.date.none.fl_str_mv 2005-11-01
2014-05-20T13:25:21Z
2014-05-20T13:25:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762005000700013
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 100, n. 7, p. 749-752, 2005.
0074-0276
http://hdl.handle.net/11449/8019
10.1590/S0074-02762005000700013
S0074-02762005000700013
S0074-02762005000700013.pdf
2114570774349859
url http://dx.doi.org/10.1590/S0074-02762005000700013
http://hdl.handle.net/11449/8019
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 100, n. 7, p. 749-752, 2005.
0074-0276
10.1590/S0074-02762005000700013
S0074-02762005000700013
S0074-02762005000700013.pdf
2114570774349859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 749-752
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129570831859712

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