Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio

Detalhes bibliográficos
Autor(a) principal: Ravagnani, Fabricio Cesar de Paula
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFMS
Texto Completo: https://repositorio.ufms.br/handle/123456789/342
Resumo: Objective: To study the effects of hypercaloric/hyperlipidic diets (chocolate and baru) associated to aerobic exercise on different fat depots as well as on substrates tissue of Wistar rats. Methods: After weaning, the animals, except the controls (NUVILAB® diet: 3.48kcal/g), were fed with a chocolate diet (4.17 kcal/g) during 2 months in order to induce obesity. After this period the animals were distributed into 6 groups: Sedentary Control (SC); Trained Control (TC); Sedentary Baru (SB); Trained Baru (TB); Sedentary Chocolate (SCho) and Trained Chocolate (TCho), according to the introduction of baru extract diet as well as of swimming training (supporting overload equivalent to 2% of body weight, during 8 wk, 5d/wk, and 1h/session). Body weight and food intake were recorded weekly. After the death of the animals, serum free fatty acids (FFA), hepatic and muscular triglycerides and glycogen (TGLmusc, TGLhep, GLICmusc, GLIChep) content were determined. The retroperitoneal (RET), inguinal (IN), and omental (OM) fat tissues were excised, weighted, and submitted to histological and adipocyte area evaluation. Results: The weight gain induced by hypercaloric/hyperlipidic diets was attenuated by exercise (SB 71.14±20,71 > TB 37.14±21,11; SCHO 54.94±18,70 > TCHO 22.30±17,14). The hypercaloric/hyperlipidic diet increased weight and cell areas of RET if compared to control diet (P<0.05). The physical training decreased the OM (TCho: 6,370.91 ± 7,776.13 < SCho: 7,341.28 ± 2.24 μm2) and IN (TCho: 5,147.49 ± 5,712.71 < SCho: 7,083.11±7,682.40 μm2) cell areas of chocolate group as well as IN cell areas of TC (TE: 2,212.87 ± 1,920.34 < SC: 3,386.11 ± 3,973.09μm2). The prevalence of hepatic steatosis was higher in sedentary rats fed with hypercaloric/hyperlipidic if compared to control group (CHOS:83%>BS:80%> CS:50%). The trained animals showed lower TGLhep content in relation to sedentary rats under continuous hypercaloric/hyperlipidic diets (SB: 1.36 ± 0.50 > TB: 0.88 ± 0.43mg.100mg-1; SCho: 1.77 ± 0.64 > TCho: 0.86 ± 0.41mg.100mg-1). The hypercaloric/hyperlipidic baru diet induced TGmusc deposition (p<0,05), in both, sedentary and trained rats, compared to controls. Conclusion: Light to moderate physical training prevented weight and TGLhep accumulation in rats fed with hypercaloric/hyperlipidic diet. Abdominal tissues were more sensitive to hyperlipidic diet compared to peripheric subcutaneous fat tissue. On the other hand, the effects of exercise were more pronounced in visceral and inguinal fat depots.
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spelling 2011-08-11T19:24:19Z2021-09-30T19:56:26Z2011https://repositorio.ufms.br/handle/123456789/342Objective: To study the effects of hypercaloric/hyperlipidic diets (chocolate and baru) associated to aerobic exercise on different fat depots as well as on substrates tissue of Wistar rats. Methods: After weaning, the animals, except the controls (NUVILAB® diet: 3.48kcal/g), were fed with a chocolate diet (4.17 kcal/g) during 2 months in order to induce obesity. After this period the animals were distributed into 6 groups: Sedentary Control (SC); Trained Control (TC); Sedentary Baru (SB); Trained Baru (TB); Sedentary Chocolate (SCho) and Trained Chocolate (TCho), according to the introduction of baru extract diet as well as of swimming training (supporting overload equivalent to 2% of body weight, during 8 wk, 5d/wk, and 1h/session). Body weight and food intake were recorded weekly. After the death of the animals, serum free fatty acids (FFA), hepatic and muscular triglycerides and glycogen (TGLmusc, TGLhep, GLICmusc, GLIChep) content were determined. The retroperitoneal (RET), inguinal (IN), and omental (OM) fat tissues were excised, weighted, and submitted to histological and adipocyte area evaluation. Results: The weight gain induced by hypercaloric/hyperlipidic diets was attenuated by exercise (SB 71.14±20,71 > TB 37.14±21,11; SCHO 54.94±18,70 > TCHO 22.30±17,14). The hypercaloric/hyperlipidic diet increased weight and cell areas of RET if compared to control diet (P<0.05). The physical training decreased the OM (TCho: 6,370.91 ± 7,776.13 < SCho: 7,341.28 ± 2.24 μm2) and IN (TCho: 5,147.49 ± 5,712.71 < SCho: 7,083.11±7,682.40 μm2) cell areas of chocolate group as well as IN cell areas of TC (TE: 2,212.87 ± 1,920.34 < SC: 3,386.11 ± 3,973.09μm2). The prevalence of hepatic steatosis was higher in sedentary rats fed with hypercaloric/hyperlipidic if compared to control group (CHOS:83%>BS:80%> CS:50%). The trained animals showed lower TGLhep content in relation to sedentary rats under continuous hypercaloric/hyperlipidic diets (SB: 1.36 ± 0.50 > TB: 0.88 ± 0.43mg.100mg-1; SCho: 1.77 ± 0.64 > TCho: 0.86 ± 0.41mg.100mg-1). The hypercaloric/hyperlipidic baru diet induced TGmusc deposition (p<0,05), in both, sedentary and trained rats, compared to controls. Conclusion: Light to moderate physical training prevented weight and TGLhep accumulation in rats fed with hypercaloric/hyperlipidic diet. Abdominal tissues were more sensitive to hyperlipidic diet compared to peripheric subcutaneous fat tissue. On the other hand, the effects of exercise were more pronounced in visceral and inguinal fat depots.Objetivo: Estudar os efeitos de dietas ricas em lipídios (chocolate e baru) e do exercício aeróbio sobre os diferentes depósitos adiposos e substratos teciduais de ratos Wistar. Métodos: Após o desmame, os animais, com exceção dos controles (dieta NUVILAB®; 3,48kcal/g), foram alimentados com dieta à base de chocolate (4,17kcal/g) por 2 meses para indução da obesidade. Feito isso, os animais foram distribuídos em 6 grupos: controle sedentários (CS), controle exercitados (CE), baru sedentários (BS), baru exercitados (BE), chocolate sedentários (CHOS), chocolate exercitados (CHOE), de acordo com a introdução da dieta com extrato de baru e treinamento de natação (2% do PC; 8 sem., 5x/semana, 1h/sessão). O ganho de peso e consumo alimentar dos animais foram registrados semanalmente. Após a morte dos animais, foram determinados: ácidos graxos livres no soro (AGLser) e o conteúdo de triglicerídeos e glicogênio muscular e hepático (TGLmusc, TGLhep, GLICmusc, GLIChep). Os tecidos adiposos retroperitoneal, inguinal e omental foram removidos, pesados e submetidos à análise histológica e área de adipócitos. Resultados: O ganho de peso corporal induzido pelas dietas hiperlipídicas/hipercalóricas foi atenuado pelo exercício físico (BS 71,14±20,71 > BE 37,14±21,11; CHOS 54,94±18,70 > CHOE 22,30±17,14). As dietas hipercalóricas/hiperlipídicas aumentaram o peso e a área de células do tecido retroperitoneal em relação à dieta controle (P<0,05). O exercício reduziu a área de células do tecido omental (CHOE: 6.370,91±7.776,13 < CHOS: 7.341,28±5.862,24μm2) e inguinal (CHOE: 5.147,49±5.712,71 BS: 80% > CS: 50%). Os animais exercitados apresentaram menor conteúdo de triglicerídeos hepáticos do que seus pares sedentários, mesmo na persistência das dietas hiperlipídicas (BS: 1,36±0,50 > BE: 0,88±0,43mg.100mg-1;CHOS: 1,77±0,64 > CHOE: 0,86±0,41mg.100mg-1). A dieta hipercalórica/hiperlipídica à base de baru promoveu maior deposição de triglicerídeos no músculo (P<0,05), em ambos, animais sedentários e exercitados, comparados aos controles. Conclusão: O treinamento físico em intensidade leve à moderada preveniu o acúmulo de peso e de TGLhep nos ratos alimentados com dietas hiperlipídicas/hipercalóricas.Os tecidos abdominais foram mais sensíveis às dietas hiperlipídicas comparados ao subcutâneo periférico. Por outro lado, os efeitos do exercício foram mais pronunciados nos depósitos adiposos visceral e inguinal.porAdipócitosDieta HiperlipídicaExercício FísicoÁrea de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbioinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisInouye, Celso MassaschiRavagnani, Fabricio Cesar de Paulainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILFabricio Cesar de Paula Ravagnani.pdf.jpgFabricio Cesar de Paula Ravagnani.pdf.jpgGenerated Thumbnailimage/jpeg1134https://repositorio.ufms.br/bitstream/123456789/342/4/Fabricio%20Cesar%20de%20Paula%20Ravagnani.pdf.jpg63a198fe2c4e912d19222aeed61a4066MD54TEXTFabricio Cesar de Paula Ravagnani.pdf.txtFabricio Cesar de Paula Ravagnani.pdf.txtExtracted texttext/plain160366https://repositorio.ufms.br/bitstream/123456789/342/3/Fabricio%20Cesar%20de%20Paula%20Ravagnani.pdf.txt7ae2c0c9cba72ba7d379d195c73288e6MD53ORIGINALFabricio Cesar de Paula Ravagnani.pdfFabricio Cesar de Paula Ravagnani.pdfapplication/pdf5436507https://repositorio.ufms.br/bitstream/123456789/342/1/Fabricio%20Cesar%20de%20Paula%20Ravagnani.pdf5c0865e0c3217f03a75a6eb2601d2dc0MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufms.br/bitstream/123456789/342/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/3422024-06-26 06:56:06.637oai:repositorio.ufms.br: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Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242024-06-26T10:56:06Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false
dc.title.pt_BR.fl_str_mv Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
title Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
spellingShingle Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
Ravagnani, Fabricio Cesar de Paula
Adipócitos
Dieta Hiperlipídica
Exercício Físico
title_short Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
title_full Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
title_fullStr Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
title_full_unstemmed Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
title_sort Área de adipócitos e parâmetros bioquímicos de ratos submetidos à dieta hiperlipídica e exercício aeróbio
author Ravagnani, Fabricio Cesar de Paula
author_facet Ravagnani, Fabricio Cesar de Paula
author_role author
dc.contributor.advisor1.fl_str_mv Inouye, Celso Massaschi
dc.contributor.author.fl_str_mv Ravagnani, Fabricio Cesar de Paula
contributor_str_mv Inouye, Celso Massaschi
dc.subject.por.fl_str_mv Adipócitos
Dieta Hiperlipídica
Exercício Físico
topic Adipócitos
Dieta Hiperlipídica
Exercício Físico
description Objective: To study the effects of hypercaloric/hyperlipidic diets (chocolate and baru) associated to aerobic exercise on different fat depots as well as on substrates tissue of Wistar rats. Methods: After weaning, the animals, except the controls (NUVILAB® diet: 3.48kcal/g), were fed with a chocolate diet (4.17 kcal/g) during 2 months in order to induce obesity. After this period the animals were distributed into 6 groups: Sedentary Control (SC); Trained Control (TC); Sedentary Baru (SB); Trained Baru (TB); Sedentary Chocolate (SCho) and Trained Chocolate (TCho), according to the introduction of baru extract diet as well as of swimming training (supporting overload equivalent to 2% of body weight, during 8 wk, 5d/wk, and 1h/session). Body weight and food intake were recorded weekly. After the death of the animals, serum free fatty acids (FFA), hepatic and muscular triglycerides and glycogen (TGLmusc, TGLhep, GLICmusc, GLIChep) content were determined. The retroperitoneal (RET), inguinal (IN), and omental (OM) fat tissues were excised, weighted, and submitted to histological and adipocyte area evaluation. Results: The weight gain induced by hypercaloric/hyperlipidic diets was attenuated by exercise (SB 71.14±20,71 > TB 37.14±21,11; SCHO 54.94±18,70 > TCHO 22.30±17,14). The hypercaloric/hyperlipidic diet increased weight and cell areas of RET if compared to control diet (P<0.05). The physical training decreased the OM (TCho: 6,370.91 ± 7,776.13 < SCho: 7,341.28 ± 2.24 μm2) and IN (TCho: 5,147.49 ± 5,712.71 < SCho: 7,083.11±7,682.40 μm2) cell areas of chocolate group as well as IN cell areas of TC (TE: 2,212.87 ± 1,920.34 < SC: 3,386.11 ± 3,973.09μm2). The prevalence of hepatic steatosis was higher in sedentary rats fed with hypercaloric/hyperlipidic if compared to control group (CHOS:83%>BS:80%> CS:50%). The trained animals showed lower TGLhep content in relation to sedentary rats under continuous hypercaloric/hyperlipidic diets (SB: 1.36 ± 0.50 > TB: 0.88 ± 0.43mg.100mg-1; SCho: 1.77 ± 0.64 > TCho: 0.86 ± 0.41mg.100mg-1). The hypercaloric/hyperlipidic baru diet induced TGmusc deposition (p<0,05), in both, sedentary and trained rats, compared to controls. Conclusion: Light to moderate physical training prevented weight and TGLhep accumulation in rats fed with hypercaloric/hyperlipidic diet. Abdominal tissues were more sensitive to hyperlipidic diet compared to peripheric subcutaneous fat tissue. On the other hand, the effects of exercise were more pronounced in visceral and inguinal fat depots.
publishDate 2011
dc.date.accessioned.fl_str_mv 2011-08-11T19:24:19Z
dc.date.issued.fl_str_mv 2011
dc.date.available.fl_str_mv 2021-09-30T19:56:26Z
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