FERRAMENTA DE BIOINFORMÁTICA PARA COMPARAÇÃO ENTRE SEQUÊNCIAS DE PROTEÍNAS

• Autor(a) principal: GILDO MATHEUS NASCIMENTO JÚNIOR
• Data de Publicação: 2024
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFMS
• Texto Completo: https://repositorio.ufms.br/handle/123456789/8947
• topic: Bioinformática; BLAST; Sequências biológicas; Ciências Exatas e da Terra

Bioinformatics is an interdisciplinary field that combines biology and computer science to analyze and interpret large sets of biological data, such as sequences of DNA, proteins, nucleotides, and amino acids. In the context of this study, the problem addressed is the need to develop effective computational tools to compare biological sequences and identify relationships between different organisms. The objective of this work is to develop a web system that allows users to compare protein or amino acid sequences, using the Basic Local Alignment Search Tool (BLAST) and the Protein Data Bank (PDB) database. The system aims to facilitate the analysis of similarities between sequences and the identification of related organisms based on these similarities. To achieve the proposed objectives, web development and integration techniques with NCBI were used, the system was implemented using the languages ​​HTML, CSS, JavaScript and PHP, as well as styling and interaction frameworks with BLAST and the PDB database. The system developed allowed users to insert protein sequences and perform searches using BLASTp. The search results were presented in a clear and intuitive way, in the format of primary sequences and 3D structures, highlighting the organisms most similar to the inserted sequences, in descending order, in percentage format. This provided users with an easy and efficient way to explore relationships between their searched sequences and sequences found in analyzed organisms, facilitating understanding of genetic diversity.