Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Monografias da UFMT |
Texto Completo: | http://bdm.ufmt.br/handle/1/1031 |
Resumo: | Strongyloidiasis is a neglected parasitic infection, usually asymptomatic and related to precarious hygiene conditions. The objective of this study was to evaluate the gastrointestinal transit of C57BL / 6 mice infected by Strongyloides venezuelensis using the Alternating Current Biosusceptometry (BAC) technique. Adult male mice were randomly divided into two groups: Control (n = 5) and Infected (n = 6) who received a parasitic load of 1500 L3 larvae. During 14 days post infection (dpi), the counting of eggs per gram of feces (OPG) was performed confirming the infection of the animals. At 7 ° and 14 ° dpi, both groups were fasted for 12 hours and then received ration with magnetic marker to record the value of their initial intensity. The percentage of Initial Excretion (first peak of magnetic intensity in the faeces relative to that of the ingested ration), percentage of Final Excretion (total excretion of feces in 10h in relation to the initial intensity of the ingested ration) was determined using the BAC technique: (TTOA), demonstrating the period that the marked ration took to transit from the mouth to the anus, the Average Gastrin-Transit Time (TMTG) over 10h. In the results the OPG showed the peak of infection in the 8dpi. The percentage of initial excretion did not differ between the control group (4.1% ± 1.3%) and the infected group in the 7dpi (4.2% ± 2.2%) and 14dpi (2.0% ± 0, 4%), demonstrating the validity of the test. The percentage of the Final Excretion showed a higher retention of the material ingested in the 7dpi Infected group (67.6% ± 26.5%) compared to Control (33.1% ± 16.2%) (p <0.05) and to 14dpi (23.3% ± 8.8%) (p <0.01). The TTOA was slowed in the Infected group 7dpi (306.4 min ± 80.4 min) compared to Control (137.1 min ± 37.3 min) (p <0.0001) and the 14dpi group (185.5 min ± 38.3 min) (p <0.01). The TMTG of the 14 dpi group (425.8 min ± 33.1min) showed an increase compared to the 7dpi group (309.0 min ± 66.9min) (p <0.05) and in relation to the control group (383.5 min ± 60.2min). It is concluded that the gastrointestinal transit of C57BL / 6 mice infected by 1500 larvae of Strongyloides venezuelensis presented changes recorded by the BAC around the peak of infection and at the end of the same. |
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Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensisCNPQ::CIENCIAS DA SAUDEBiosusceptometria de corrente alternada (BAC)EstrongiloidíaseMurinosOvos por grama de fezes (OPG)Alternating current biosusceptometry (BAC)StrongyloidiasisMurineEggs per gram of faeces (OPG)Strongyloidiasis is a neglected parasitic infection, usually asymptomatic and related to precarious hygiene conditions. The objective of this study was to evaluate the gastrointestinal transit of C57BL / 6 mice infected by Strongyloides venezuelensis using the Alternating Current Biosusceptometry (BAC) technique. Adult male mice were randomly divided into two groups: Control (n = 5) and Infected (n = 6) who received a parasitic load of 1500 L3 larvae. During 14 days post infection (dpi), the counting of eggs per gram of feces (OPG) was performed confirming the infection of the animals. At 7 ° and 14 ° dpi, both groups were fasted for 12 hours and then received ration with magnetic marker to record the value of their initial intensity. The percentage of Initial Excretion (first peak of magnetic intensity in the faeces relative to that of the ingested ration), percentage of Final Excretion (total excretion of feces in 10h in relation to the initial intensity of the ingested ration) was determined using the BAC technique: (TTOA), demonstrating the period that the marked ration took to transit from the mouth to the anus, the Average Gastrin-Transit Time (TMTG) over 10h. In the results the OPG showed the peak of infection in the 8dpi. The percentage of initial excretion did not differ between the control group (4.1% ± 1.3%) and the infected group in the 7dpi (4.2% ± 2.2%) and 14dpi (2.0% ± 0, 4%), demonstrating the validity of the test. The percentage of the Final Excretion showed a higher retention of the material ingested in the 7dpi Infected group (67.6% ± 26.5%) compared to Control (33.1% ± 16.2%) (p <0.05) and to 14dpi (23.3% ± 8.8%) (p <0.01). The TTOA was slowed in the Infected group 7dpi (306.4 min ± 80.4 min) compared to Control (137.1 min ± 37.3 min) (p <0.0001) and the 14dpi group (185.5 min ± 38.3 min) (p <0.01). The TMTG of the 14 dpi group (425.8 min ± 33.1min) showed an increase compared to the 7dpi group (309.0 min ± 66.9min) (p <0.05) and in relation to the control group (383.5 min ± 60.2min). It is concluded that the gastrointestinal transit of C57BL / 6 mice infected by 1500 larvae of Strongyloides venezuelensis presented changes recorded by the BAC around the peak of infection and at the end of the same.Estrongiloidíase é uma infecção parasitária negligenciada, geralmente assintomática e relacionada a condições de higiene precárias. Objetivou-se avaliar o trânsito gastrintestinal de camundongos C57BL/6 infectados por Strongyloides venezuelensis por meio da técnica de Biosusceptometria de Corrente Alternada (BAC). Foram utilizados camundongos machos adultos, divididos aleatoriamente em dois grupos: Controle (n=5) e Infectados (n=6) que receberam uma carga parasitária de 1500 larvas L3. Ao longo de 14 dias pós infecção (dpi) foi realizada a técnica de contagem de ovos por gramas de fezes (OPG) confirmando a infecção dos animais. No 7° e 14° dpi, ambos os grupos ficaram em jejum por 12 horas e depois receberam ração com marcador magnético para registro do valor de sua intensidade inicial. Foram determinados utilizando a técnica de BAC: a percentagem de Excreção Inicial (primeiro pico de intensidade magnética nas fezes em relação a da ração ingerida), percentagem de Excreção Final (excreção total de fezes em 10h em relação a intensidade inicial da ração ingerida), o Tempo de Trânsito oro-anal (TTOA) demonstrando o período que a ração marcada levou para transitar da boca ao ânus, o Tempo Médio de Trânsito Gastrintestinal (TMTG) ao longo de 10h. Nos resultados a OPG, apresentou o pico de infecção no 8dpi. A Percentagem da Excreção Inicial não apresentou diferença entre o grupo Controle (4,1%±1,3%) e o Infectado no 7dpi (4,2%±2,2%) e no 14dpi (2,0%±0,4%), demonstrando a validade do teste. A Percentagem da Excreção Final demonstrou maior retenção do material ingerido no grupo Infectado no 7dpi (67,6%±26,5%) em relação ao Controle (33,1%±16,2%) (p<0,05) e ao 14dpi (23,3%±8,8%) (p<0,01). O TTOA foi lentificado no grupo Infectado 7dpi (306,4 min±80,4 min) comparado ao Controle (137,1 min±37,3 min) (p<0,0001) e ao grupo 14dpi (185,5 min±38,3 min) (p<0,01). O TMTG do grupo 14 dpi (425,8 min±33,1min) demostrou aumento comparado ao grupo 7dpi (309,0 min±66,9min) (p<0,05) e em relação ao controle (383,5 min±60,2min). Conclui-se que o trânsito gastrintestinal dos camundongos C57BL/6 infectados por 1500 larvas de Strongyloides venezuelensis apresentou alterações registradas pela BAC em torno do pico de infecção e ao termino da mesma.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Biológicas e da Saúde (ICBS) – AraguaiaUFMT CUA - AraguaiaBiomedicina - CUAAmérico, Madileine Francelyhttp://lattes.cnpq.br/4097128727139521Américo, Madileine Francelyhttp://lattes.cnpq.br/4097128727139521Ramos , Luana dos Anjoshttp://lattes.cnpq.br/6674750361624537Almeida, Maria Aparecida Sousa Oliveirahttp://lattes.cnpq.br/4931485464790817Alves, Wellington David Luz2019-05-21T07:41:32Z2019-04-122019-05-21T07:41:32Z2019-03-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/datasetALVES, Wellington David Luz. Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis. 2019. 38 f. TCC (Graduação em Biomedicina) - Universidade Federal do Mato Grosso, Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2019.http://bdm.ufmt.br/handle/1/1031porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Monografias da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2019-05-23T07:00:28Zoai:localhost:1/1031Biblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestopendoar:2019-05-23T07:00:28falseBiblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestbibliotecacentral@ufmt.br||opendoar:2019-05-23T07:00:28Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis |
title |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis |
spellingShingle |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis Alves, Wellington David Luz CNPQ::CIENCIAS DA SAUDE Biosusceptometria de corrente alternada (BAC) Estrongiloidíase Murinos Ovos por grama de fezes (OPG) Alternating current biosusceptometry (BAC) Strongyloidiasis Murine Eggs per gram of faeces (OPG) |
title_short |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis |
title_full |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis |
title_fullStr |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis |
title_full_unstemmed |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis |
title_sort |
Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis |
author |
Alves, Wellington David Luz |
author_facet |
Alves, Wellington David Luz |
author_role |
author |
dc.contributor.none.fl_str_mv |
Américo, Madileine Francely http://lattes.cnpq.br/4097128727139521 Américo, Madileine Francely http://lattes.cnpq.br/4097128727139521 Ramos , Luana dos Anjos http://lattes.cnpq.br/6674750361624537 Almeida, Maria Aparecida Sousa Oliveira http://lattes.cnpq.br/4931485464790817 |
dc.contributor.author.fl_str_mv |
Alves, Wellington David Luz |
dc.subject.por.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE Biosusceptometria de corrente alternada (BAC) Estrongiloidíase Murinos Ovos por grama de fezes (OPG) Alternating current biosusceptometry (BAC) Strongyloidiasis Murine Eggs per gram of faeces (OPG) |
topic |
CNPQ::CIENCIAS DA SAUDE Biosusceptometria de corrente alternada (BAC) Estrongiloidíase Murinos Ovos por grama de fezes (OPG) Alternating current biosusceptometry (BAC) Strongyloidiasis Murine Eggs per gram of faeces (OPG) |
description |
Strongyloidiasis is a neglected parasitic infection, usually asymptomatic and related to precarious hygiene conditions. The objective of this study was to evaluate the gastrointestinal transit of C57BL / 6 mice infected by Strongyloides venezuelensis using the Alternating Current Biosusceptometry (BAC) technique. Adult male mice were randomly divided into two groups: Control (n = 5) and Infected (n = 6) who received a parasitic load of 1500 L3 larvae. During 14 days post infection (dpi), the counting of eggs per gram of feces (OPG) was performed confirming the infection of the animals. At 7 ° and 14 ° dpi, both groups were fasted for 12 hours and then received ration with magnetic marker to record the value of their initial intensity. The percentage of Initial Excretion (first peak of magnetic intensity in the faeces relative to that of the ingested ration), percentage of Final Excretion (total excretion of feces in 10h in relation to the initial intensity of the ingested ration) was determined using the BAC technique: (TTOA), demonstrating the period that the marked ration took to transit from the mouth to the anus, the Average Gastrin-Transit Time (TMTG) over 10h. In the results the OPG showed the peak of infection in the 8dpi. The percentage of initial excretion did not differ between the control group (4.1% ± 1.3%) and the infected group in the 7dpi (4.2% ± 2.2%) and 14dpi (2.0% ± 0, 4%), demonstrating the validity of the test. The percentage of the Final Excretion showed a higher retention of the material ingested in the 7dpi Infected group (67.6% ± 26.5%) compared to Control (33.1% ± 16.2%) (p <0.05) and to 14dpi (23.3% ± 8.8%) (p <0.01). The TTOA was slowed in the Infected group 7dpi (306.4 min ± 80.4 min) compared to Control (137.1 min ± 37.3 min) (p <0.0001) and the 14dpi group (185.5 min ± 38.3 min) (p <0.01). The TMTG of the 14 dpi group (425.8 min ± 33.1min) showed an increase compared to the 7dpi group (309.0 min ± 66.9min) (p <0.05) and in relation to the control group (383.5 min ± 60.2min). It is concluded that the gastrointestinal transit of C57BL / 6 mice infected by 1500 larvae of Strongyloides venezuelensis presented changes recorded by the BAC around the peak of infection and at the end of the same. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-21T07:41:32Z 2019-04-12 2019-05-21T07:41:32Z 2019-03-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis info:eu-repo/semantics/dataset |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ALVES, Wellington David Luz. Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis. 2019. 38 f. TCC (Graduação em Biomedicina) - Universidade Federal do Mato Grosso, Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2019. http://bdm.ufmt.br/handle/1/1031 |
identifier_str_mv |
ALVES, Wellington David Luz. Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis. 2019. 38 f. TCC (Graduação em Biomedicina) - Universidade Federal do Mato Grosso, Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2019. |
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http://bdm.ufmt.br/handle/1/1031 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Mato Grosso Brasil Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia UFMT CUA - Araguaia Biomedicina - CUA |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia UFMT CUA - Araguaia Biomedicina - CUA |
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reponame:Biblioteca Digital de Monografias da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
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Universidade Federal de Mato Grosso (UFMT) |
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UFMT |
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Biblioteca Digital de Monografias da UFMT |
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Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT) |
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