Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Monografias da UFMT |
Texto Completo: | http://bdm.ufmt.br/handle/1/1900 |
Resumo: | Post-translational modifications (MPTs) occur throughout the body and are essential for protein function. The reversible addition or removal of chemical groups in a protein implies changes in its activity or location. Among these MPTs, O-glycosylation with Nacetylglucosamine (O-GlcNAc) stands out. Several proteins are targets of O-GlcNAc, including proteins responsible for regulating vascular tone. It is also known that males and females have vascular differences associated with hormonal activity in the vessels. Therefore, the aim of this study was to evaluate whether chronic treatment with glucosamine modulates the vascular response in the aorta of males and females differently. Male Wistar rats (13-15 wk; 350-400g) and females (13-15 wk; 300-350g) were randomly divided into the following groups a) vehicle males, b) glucosamine males, c) vehicle females and d) females glucosamine, where the groups treated with glucosamine received a daily dose of 300 mg / kg for 21 days (ip, n = 15) and the control group received saline daily for 21 days (ip, n = 15). At the end of treatment, blood pressure measurement and oral glucose tolerance test were performed. Concentration-response curves (CRC) for acetylcholine (ACh), phenylephrine (PHE) and sodium nitroprusside [SNP (in the presence of L-NAME; 100 µM, 30 min.)] were performed in the aorta. Glucosamine treatment for 21 days did not alter systolic blood pressure or glucose tolerance in any group. However, CRC for PHE demonstrated a decrease in contractile response in the male group treated with glucosamine compared to the control group [Emax (%) 61.43 ± 3.76 vs. 88.20 ± 2.14, respectively]. Endothelium-dependent relaxation was greater in the male glucosamine group compared to the control group [pD2 (%) 7.63 ± 0.09 vs. 7.12 ± 0.11, respectively] without changing the maximum response. In females there were no significant changes for PHE or Ach. Endothelium-independent relaxation was also not altered in any group. The data found suggest that treatment with glucosamine for 21 days results in vascular dysfunction via decreased contractile response in the aorta of males, while females have protection against glucosamine. |
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Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistarCNPQ::OUTROS::BIOMEDICINAContraçãoEndotélioGlicosilaçãoMúsculo Liso VascularO-GlcNAcContractionEndotheliumGlycosylationVascular smooth muscleO-GlcNAcPost-translational modifications (MPTs) occur throughout the body and are essential for protein function. The reversible addition or removal of chemical groups in a protein implies changes in its activity or location. Among these MPTs, O-glycosylation with Nacetylglucosamine (O-GlcNAc) stands out. Several proteins are targets of O-GlcNAc, including proteins responsible for regulating vascular tone. It is also known that males and females have vascular differences associated with hormonal activity in the vessels. Therefore, the aim of this study was to evaluate whether chronic treatment with glucosamine modulates the vascular response in the aorta of males and females differently. Male Wistar rats (13-15 wk; 350-400g) and females (13-15 wk; 300-350g) were randomly divided into the following groups a) vehicle males, b) glucosamine males, c) vehicle females and d) females glucosamine, where the groups treated with glucosamine received a daily dose of 300 mg / kg for 21 days (ip, n = 15) and the control group received saline daily for 21 days (ip, n = 15). At the end of treatment, blood pressure measurement and oral glucose tolerance test were performed. Concentration-response curves (CRC) for acetylcholine (ACh), phenylephrine (PHE) and sodium nitroprusside [SNP (in the presence of L-NAME; 100 µM, 30 min.)] were performed in the aorta. Glucosamine treatment for 21 days did not alter systolic blood pressure or glucose tolerance in any group. However, CRC for PHE demonstrated a decrease in contractile response in the male group treated with glucosamine compared to the control group [Emax (%) 61.43 ± 3.76 vs. 88.20 ± 2.14, respectively]. Endothelium-dependent relaxation was greater in the male glucosamine group compared to the control group [pD2 (%) 7.63 ± 0.09 vs. 7.12 ± 0.11, respectively] without changing the maximum response. In females there were no significant changes for PHE or Ach. Endothelium-independent relaxation was also not altered in any group. The data found suggest that treatment with glucosamine for 21 days results in vascular dysfunction via decreased contractile response in the aorta of males, while females have protection against glucosamine.As modificações pós-traducionais (MPTs) ocorrem em todo o organismo e são essenciais para função proteica. A adição ou remoção reversível de grupamentos químicos em uma proteína implica mudanças em sua atividade ou localização. Dentre essas MPTs, destacase a O-glicosilação com N-acetilglucosamina (O-GlcNAc). Diversas proteínas são alvo da OGlcNAc, incluindo as proteínas responsáveis pela regulação do tônus vascular. Sabe-se também que machos e fêmeas possuem diferenças vasculares associadas à atividade hormonal nos vasos. Portanto, o objetivo deste estudo foi avaliar se o tratamento crônico com glucosamina modula diferentemente a resposta vascular na aorta de machos e fêmeas. Ratos Wistar machos (13-15 sem.; 350-400g) e fêmeas (13-15 sem.; 300-350g) foram divididos aleatoriamente entre os grupos a) machos veículo, b) machos glucosamina, c) fêmeas veículo e d) fêmeas glucosamina, onde os grupos tratados com glucosamina receberam dose diária de 300 mg/kg por 21 dias (i.p., n= 15) e o grupo controle recebeu salina diariamente por 21 dias (i.p, n= 15). Ao final do tratamento foram realizados aferição da pressão arterial caudal e teste de tolerância oral à glicose. Curvas concentração-resposta (CCR) para acetilcolina (ACh), fenilefrina (FE) e nitroprussiato de sódio [NPS (na presença de L-NAME; 100 µM, 30 min.)] foram realizados na aorta. O tratamento com glucosamina por 21 dias não alterou a pressão arterial sistólica bem como a tolerância a glicose de nenhum grupo. Entretanto, CCR para FE demonstrou diminuição da resposta contrátil no grupo machos tratados com glucosamina em comparação com o grupo controle [Emax (%) 61,43 ± 3,76 vs. 88,20 ± 2,14, respectivamente]. Relaxamento dependente de endotélio foi maior no grupo machos glucosamina em relação ao controle [pD2 (%) 7,63 ± 0,09 vs. 7,12 ± 0,11, respectivamente] sem alteração da resposta máxima. Nas fêmeas não houveram alterações significativas para FE ou Ach. O relaxamento independente do endotélio também não foi alterado em nenhum grupo. Os dados encontrados sugerem que o tratamento com glucosamina por 21 dias resulta em disfunção vascular via diminuição de resposta contrátil na aorta de machos, enquanto as fêmeas apresentam uma proteção frente à glucosamina.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Biológicas e da Saúde (ICBS) – AraguaiaUFMT CUA - AraguaiaBiomedicina - CUASouto, Paula Cristina de Souzahttp://lattes.cnpq.br/2161765071682282Souto, Paula Cristina de Souzahttp://lattes.cnpq.br/2161765071682282Souza, Kamilla Batista Da Silva2021-07-04T20:40:30Z2020-03-102021-07-04T20:40:30Z2020-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/datasetSOUZA, Kamilla Batista Da Silva. Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar. 2020. 34 f. TCC (Graduação em Biomedicina) - Universidade Federal do Mato Grosso, Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2020.http://bdm.ufmt.br/handle/1/1900porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Monografias da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2021-07-06T07:00:21Zoai:localhost:1/1900Biblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestopendoar:2021-07-06T07:00:21falseBiblioteca Digital de Monografiahttps://bdm.ufmt.br/PUBhttp://200.129.241.122/oai/requestbibliotecacentral@ufmt.br||opendoar:2021-07-06T07:00:21Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar |
title |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar |
spellingShingle |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar Souza, Kamilla Batista Da Silva CNPQ::OUTROS::BIOMEDICINA Contração Endotélio Glicosilação Músculo Liso Vascular O-GlcNAc Contraction Endothelium Glycosylation Vascular smooth muscle O-GlcNAc |
title_short |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar |
title_full |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar |
title_fullStr |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar |
title_full_unstemmed |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar |
title_sort |
Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar |
author |
Souza, Kamilla Batista Da Silva |
author_facet |
Souza, Kamilla Batista Da Silva |
author_role |
author |
dc.contributor.none.fl_str_mv |
Souto, Paula Cristina de Souza http://lattes.cnpq.br/2161765071682282 Souto, Paula Cristina de Souza http://lattes.cnpq.br/2161765071682282 |
dc.contributor.author.fl_str_mv |
Souza, Kamilla Batista Da Silva |
dc.subject.por.fl_str_mv |
CNPQ::OUTROS::BIOMEDICINA Contração Endotélio Glicosilação Músculo Liso Vascular O-GlcNAc Contraction Endothelium Glycosylation Vascular smooth muscle O-GlcNAc |
topic |
CNPQ::OUTROS::BIOMEDICINA Contração Endotélio Glicosilação Músculo Liso Vascular O-GlcNAc Contraction Endothelium Glycosylation Vascular smooth muscle O-GlcNAc |
description |
Post-translational modifications (MPTs) occur throughout the body and are essential for protein function. The reversible addition or removal of chemical groups in a protein implies changes in its activity or location. Among these MPTs, O-glycosylation with Nacetylglucosamine (O-GlcNAc) stands out. Several proteins are targets of O-GlcNAc, including proteins responsible for regulating vascular tone. It is also known that males and females have vascular differences associated with hormonal activity in the vessels. Therefore, the aim of this study was to evaluate whether chronic treatment with glucosamine modulates the vascular response in the aorta of males and females differently. Male Wistar rats (13-15 wk; 350-400g) and females (13-15 wk; 300-350g) were randomly divided into the following groups a) vehicle males, b) glucosamine males, c) vehicle females and d) females glucosamine, where the groups treated with glucosamine received a daily dose of 300 mg / kg for 21 days (ip, n = 15) and the control group received saline daily for 21 days (ip, n = 15). At the end of treatment, blood pressure measurement and oral glucose tolerance test were performed. Concentration-response curves (CRC) for acetylcholine (ACh), phenylephrine (PHE) and sodium nitroprusside [SNP (in the presence of L-NAME; 100 µM, 30 min.)] were performed in the aorta. Glucosamine treatment for 21 days did not alter systolic blood pressure or glucose tolerance in any group. However, CRC for PHE demonstrated a decrease in contractile response in the male group treated with glucosamine compared to the control group [Emax (%) 61.43 ± 3.76 vs. 88.20 ± 2.14, respectively]. Endothelium-dependent relaxation was greater in the male glucosamine group compared to the control group [pD2 (%) 7.63 ± 0.09 vs. 7.12 ± 0.11, respectively] without changing the maximum response. In females there were no significant changes for PHE or Ach. Endothelium-independent relaxation was also not altered in any group. The data found suggest that treatment with glucosamine for 21 days results in vascular dysfunction via decreased contractile response in the aorta of males, while females have protection against glucosamine. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-10 2020-02-19 2021-07-04T20:40:30Z 2021-07-04T20:40:30Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis info:eu-repo/semantics/dataset |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUZA, Kamilla Batista Da Silva. Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar. 2020. 34 f. TCC (Graduação em Biomedicina) - Universidade Federal do Mato Grosso, Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2020. http://bdm.ufmt.br/handle/1/1900 |
identifier_str_mv |
SOUZA, Kamilla Batista Da Silva. Diferenças sexuais na reatividade vascular da aorta após tratamento com glucosamina em ratos wistar. 2020. 34 f. TCC (Graduação em Biomedicina) - Universidade Federal do Mato Grosso, Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2020. |
url |
http://bdm.ufmt.br/handle/1/1900 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia UFMT CUA - Araguaia Biomedicina - CUA |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia UFMT CUA - Araguaia Biomedicina - CUA |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Monografias da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
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Universidade Federal de Mato Grosso (UFMT) |
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UFMT |
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UFMT |
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Biblioteca Digital de Monografias da UFMT |
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Biblioteca Digital de Monografias da UFMT |
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Biblioteca Digital de Monografias da UFMT - Universidade Federal de Mato Grosso (UFMT) |
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