Estudo fitoquímico das folhas de Duguetia furfuracea (Annonaceae) e avaliação preliminar antileucêmica

• Autor(a) principal: Godoy, Yasmin Aparecida Martins de
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Biblioteca Digital de Monografias da UFMT
• Texto Completo: http://bdm.ufmt.br/handle/1/3668
• topic: CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA; Duguetia furfuracea; Flavonoide; Canferol; Isoramnetina; Antileucêmica; Duguetia furfuracea; Flavonoid; Kaempferol; Isoramnetin; Antileukemic

Duguetia furfuracea is a shrub belonging to the Annonaceae family, commonlyfound in tropical and subtropical climates, occurring widely in the Braziliancerrado, which has 33 of the 135 genera already cataloged. Popularly knownas"dryaraticum", "ata do bush" or "suffer from the kidney who wants", in popular medicinethe teas of its leaves are used to treat dysmenorrhea, diarrhea and rheumatism. Chemical studies described in the literature for the species indicatethepredominance of some special metabolites such as sesquiterpenes, alkaloids, flavonoids, tannins, acetogenins, and these are mentioned in a wide spectrumof biological activities, among which stand out the potential anticancer, antimalarial, antitumor , diuretics, antivirals, anti-inflammatories, antimicrobials, antiviralsandantioxidants. In this work, the phytochemical study of the leaves of D. furfuraceawas carried out through the fractionation of the chloroform(FCHCl3) andethyl acetate (FAcoet) fractions. From FCHCl3, after successive fractionationsinaclassic column (CC), resulted in the isolation of an amorphous yellowsolid, identified as the flavonoid kaempferol. The ethyl acetate fraction (FAcoet) wassubmitted to classical chromatography and recrystallization, obtainingayellowprecipitate, identified as the flavonoid isoramnetin. The structural identificationof the substances was made after analysis of the 1D and 2D NMRspectra, andcomparison with literature data. The antileukemic activity assay was performedthrough cytotoxic screening against human leukemia cell lines in Kasumi-1andK-562 cells. Samples of the crude extract of the leaves and their respectivefractions(FHex, FCHCl3, FAcoet and hydromethanol residue) were tested, and theFHex, FCHCl3 and FAcoet showed promising results. Despite the various phytochemical studies described in the literature on D. furfuracea, this is the first timethat theflavonoid kaempferol is reported in the species, thus contributingtothechemotaxonomy of the species.