Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMT |
Texto Completo: | http://ri.ufmt.br/handle/1/2996 |
Resumo: | Because of the nutritional transition, increased intake of foods with high energy content and decreased of physical activity the prevalence of chronic diseases increased worldwide. Many of these foods contain a large amounts of fructose, that was used to replace saccharose as a sweetener after the 60s. It is well known that primary lymphoid organs, bone marrow and thymus, are responsible for the formation and maturation of cells that act during immune responses. Therefore, this study focuses on the role of fructose on physiological, biochemical, nutritional parameters and on primary lymphoid organs of Wistar rats. Male Wistar rats were used between 6 to 8 weeks of age and were treated with fructose (7%) dissolved in water for 12 weeks. After this period the animals were killed for analysis of anthropometric, biochemical, immunological and histopathological parameters. Food intake was higher in the control group compared to fructose group, however it was consumed in total more energy by the fructose group (water with fructose + food). The animals of both groups showed greater weight gain in the first weeks of treatment, but the fructose group showed more weight variations. The glycemic levels was increased in the fructose group at 1st month compared to control. When compared the fructose group with each other, the blood glucose was lower in 3 th month vs 1st month. There were no significantly difference on the lipid profile, leukogram and relative weight of the thymus between groups. In contrast there was an increase on cellularity of the thymus and the bone marrow, as confirmed by the histological sections analysis. The myelogram showed an increased production of eosinophils and lymphocytes and a decreased of rubroblastos, pro-rubrócitos and neutrophils in the fructose group. Regarding the architecture of the lymphoid organs, there were no changes in the bone marrow, however the thymus showed a medullary disorder and infiltration of histiocytes in thymic cortex in the fructose group. In conclusion, the treatment with 7% fructose was not sufficient to cause hyperglycemia or dyslipidemia in the animals, however it modulates the production of cells by the bone marrow and caused changes in thymic structures. |
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Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutoseFrutoseTimoMedula ósseaCNPQ::CIENCIAS DA SAUDEFructoseThymusBone marrowBecause of the nutritional transition, increased intake of foods with high energy content and decreased of physical activity the prevalence of chronic diseases increased worldwide. Many of these foods contain a large amounts of fructose, that was used to replace saccharose as a sweetener after the 60s. It is well known that primary lymphoid organs, bone marrow and thymus, are responsible for the formation and maturation of cells that act during immune responses. Therefore, this study focuses on the role of fructose on physiological, biochemical, nutritional parameters and on primary lymphoid organs of Wistar rats. Male Wistar rats were used between 6 to 8 weeks of age and were treated with fructose (7%) dissolved in water for 12 weeks. After this period the animals were killed for analysis of anthropometric, biochemical, immunological and histopathological parameters. Food intake was higher in the control group compared to fructose group, however it was consumed in total more energy by the fructose group (water with fructose + food). The animals of both groups showed greater weight gain in the first weeks of treatment, but the fructose group showed more weight variations. The glycemic levels was increased in the fructose group at 1st month compared to control. When compared the fructose group with each other, the blood glucose was lower in 3 th month vs 1st month. There were no significantly difference on the lipid profile, leukogram and relative weight of the thymus between groups. In contrast there was an increase on cellularity of the thymus and the bone marrow, as confirmed by the histological sections analysis. The myelogram showed an increased production of eosinophils and lymphocytes and a decreased of rubroblastos, pro-rubrócitos and neutrophils in the fructose group. Regarding the architecture of the lymphoid organs, there were no changes in the bone marrow, however the thymus showed a medullary disorder and infiltration of histiocytes in thymic cortex in the fructose group. In conclusion, the treatment with 7% fructose was not sufficient to cause hyperglycemia or dyslipidemia in the animals, however it modulates the production of cells by the bone marrow and caused changes in thymic structures.Em ocorrência da transição nutricional, aumento de ingesta de alimentos com alto teor energético e diminuição da prática de atividades físicas, aumentou-se a prevalência de doenças crônicas na população mundial. Muitos desses alimentos consumidos possuem grande quantidade de frutose, sendo que a mesma substituiu a sacarose, como adoçante, depois da década de 60. Sabe-se que os órgãos linfóides primários, timo e medula óssea, são responsáveis pela formação e maturação de células que atuam durantes as respostas imunes. O bom funcionamento do sistema imunológico depende de vários nutrientes que têm papel chave no organismo e a má nutrição pode trazer prejuízos como a deficiência do sistema imunológico. Com isso, objetivou-se estudar a atuação da frutose sobre parâmetros fisiológicos, bioquímicos, nutricionais e em órgãos linfóides primários de ratos Wistar. Para tanto, foram utilizados ratos Wistar, entre 6 a 8 semanas de vida, tratados com frutose (7%), dissolvida em água, durante 12 semanas. Após esse período os animais foram eutanasiados para posterior análise de parâmetros antropométricos, bioquímicos, imunológicos e também histopatológicos do timo e da medula óssea. A ingesta de energia vinda da ração foi maior no grupo controle comparado à frutose, no entanto foi consumido, no total, maior energia pelo grupo frutose (água com frutose + ração). Os animais apresentaram maior ganho de peso nas primeiras semanas de tratamento em ambos os grupos, porém o grupo frutose apresentou maiores variações de peso. O valor glicêmico, entre os grupos, foi aumentado no grupo frutose no mês 1 e comparando dentro do mesmo grupo houve diminuição da glicemia no mês 3 vs mês 1. O lipidograma, leucograma e peso relativo do timo não apresentaram alterações significativas entre os grupos. Entretanto, houve aumento de celularidade tanto no timo quanto na medula óssea sendo confirmada pela análise dos cortes histológicos e no mielograma observou-se aumento da produção de eosinófilos e linfócitos e diminuição de rubroblastos, pró-rubrócitos, neutrófilos e bastonetes no grupo frutose. Quanto à arquitetura dos órgãos linfóides, não houve alterações na medula óssea, porém no timo, houve desarranjo medular e infiltrado de histiócitos no córtex tímico dos animais tratados com frutose. Conclui- se que o tratamento com 7% de frutose não foi suficiente para causar hiperglicemia ou dislipidemia nos animais, porém, modulou a produção de células pela medula óssea e causou modificações nas estruturas do timo.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Biológicas e da Saúde (ICBS) – AraguaiaUFMT CUA - AraguaiaPrograma de Pós-Graduação em Imunologia e Parasitologia Básicas e AplicadasSouto, Paula Cristina de SouzaCampos, Kleber Eduardo dehttp://lattes.cnpq.br/6939343303863367http://lattes.cnpq.br/2161765071682282Souto, Paula Cristina de Souza277.680.138-10http://lattes.cnpq.br/2161765071682282Brune, Maria Fernanda Spegiorin Salla025.278.569-00http://lattes.cnpq.br/6490966175710971277.680.138-10270.119.548-99Reinaque, Ana Paula Barcelos256.830.168-60http://lattes.cnpq.br/6456302022066024Schönholzer, Tatiele Estefâni2021-09-27T12:39:35Z2016-03-212021-09-27T12:39:35Z2016-03-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSCHÖNHOLZER, Tatiele Estefâni. Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose. 2016. 46 f. Dissertação (Mestrado em Imunologia e Parasitologia Básicas e Aplicadas) - Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2016.http://ri.ufmt.br/handle/1/2996porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2021-09-28T07:03:34Zoai:localhost:1/2996Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2021-09-28T07:03:34Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose |
title |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose |
spellingShingle |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose Schönholzer, Tatiele Estefâni Frutose Timo Medula óssea CNPQ::CIENCIAS DA SAUDE Fructose Thymus Bone marrow |
title_short |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose |
title_full |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose |
title_fullStr |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose |
title_full_unstemmed |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose |
title_sort |
Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose |
author |
Schönholzer, Tatiele Estefâni |
author_facet |
Schönholzer, Tatiele Estefâni |
author_role |
author |
dc.contributor.none.fl_str_mv |
Souto, Paula Cristina de Souza Campos, Kleber Eduardo de http://lattes.cnpq.br/6939343303863367 http://lattes.cnpq.br/2161765071682282 Souto, Paula Cristina de Souza 277.680.138-10 http://lattes.cnpq.br/2161765071682282 Brune, Maria Fernanda Spegiorin Salla 025.278.569-00 http://lattes.cnpq.br/6490966175710971 277.680.138-10 270.119.548-99 Reinaque, Ana Paula Barcelos 256.830.168-60 http://lattes.cnpq.br/6456302022066024 |
dc.contributor.author.fl_str_mv |
Schönholzer, Tatiele Estefâni |
dc.subject.por.fl_str_mv |
Frutose Timo Medula óssea CNPQ::CIENCIAS DA SAUDE Fructose Thymus Bone marrow |
topic |
Frutose Timo Medula óssea CNPQ::CIENCIAS DA SAUDE Fructose Thymus Bone marrow |
description |
Because of the nutritional transition, increased intake of foods with high energy content and decreased of physical activity the prevalence of chronic diseases increased worldwide. Many of these foods contain a large amounts of fructose, that was used to replace saccharose as a sweetener after the 60s. It is well known that primary lymphoid organs, bone marrow and thymus, are responsible for the formation and maturation of cells that act during immune responses. Therefore, this study focuses on the role of fructose on physiological, biochemical, nutritional parameters and on primary lymphoid organs of Wistar rats. Male Wistar rats were used between 6 to 8 weeks of age and were treated with fructose (7%) dissolved in water for 12 weeks. After this period the animals were killed for analysis of anthropometric, biochemical, immunological and histopathological parameters. Food intake was higher in the control group compared to fructose group, however it was consumed in total more energy by the fructose group (water with fructose + food). The animals of both groups showed greater weight gain in the first weeks of treatment, but the fructose group showed more weight variations. The glycemic levels was increased in the fructose group at 1st month compared to control. When compared the fructose group with each other, the blood glucose was lower in 3 th month vs 1st month. There were no significantly difference on the lipid profile, leukogram and relative weight of the thymus between groups. In contrast there was an increase on cellularity of the thymus and the bone marrow, as confirmed by the histological sections analysis. The myelogram showed an increased production of eosinophils and lymphocytes and a decreased of rubroblastos, pro-rubrócitos and neutrophils in the fructose group. Regarding the architecture of the lymphoid organs, there were no changes in the bone marrow, however the thymus showed a medullary disorder and infiltration of histiocytes in thymic cortex in the fructose group. In conclusion, the treatment with 7% fructose was not sufficient to cause hyperglycemia or dyslipidemia in the animals, however it modulates the production of cells by the bone marrow and caused changes in thymic structures. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-21 2016-03-21 2021-09-27T12:39:35Z 2021-09-27T12:39:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SCHÖNHOLZER, Tatiele Estefâni. Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose. 2016. 46 f. Dissertação (Mestrado em Imunologia e Parasitologia Básicas e Aplicadas) - Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2016. http://ri.ufmt.br/handle/1/2996 |
identifier_str_mv |
SCHÖNHOLZER, Tatiele Estefâni. Avaliação de órgãos linfóides primários de ratos Wistar tratados com frutose. 2016. 46 f. Dissertação (Mestrado em Imunologia e Parasitologia Básicas e Aplicadas) - Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2016. |
url |
http://ri.ufmt.br/handle/1/2996 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia UFMT CUA - Araguaia Programa de Pós-Graduação em Imunologia e Parasitologia Básicas e Aplicadas |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia UFMT CUA - Araguaia Programa de Pós-Graduação em Imunologia e Parasitologia Básicas e Aplicadas |
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reponame:Repositório Institucional da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
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Universidade Federal de Mato Grosso (UFMT) |
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Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT) |
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jordanbiblio@gmail.com |
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