Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMT |
Texto Completo: | http://ri.ufmt.br/handle/1/1625 |
Resumo: | The paper presents the chemical and biological study of seed and stem bark of Hymenaea courbaril L. var coubaril. Chemical studies of the seed resulted in the isolation of two biscoumarins: biscoumarin-7-O-β-glucopyranoside-2'-O-α-apiofuranosyl-2'''-O-α-galactopyranoside and 7-O-biscoumarin-β-glucopyranoside-2'-O-α-apiofuranosyl, elucidated by 1H NMR, 13C HMBC, HSQC spectrometry and high resolution mass. The chemical composition of the seeds oil was quantified by GC-MS and it identified the presence of palmitic (11.9%), linoleic (30.6%), arachidic (3.7%) behenic (9.2%) and lignoceric acid (5.8%). With respect to the DPPH• test, the residue of the EtOH extract of the stem bark showed the best antioxidant potential with 97.77%, followed by EtOH extract of the bark (97.71%), EtOH residue of the seeds (96.10%) and AcOEt extract of the seeds (94.90%). The biological study of the oil from the seeds has been done to investigate the antimicrobial activity using standard microorganisms (ATCC strains) with: Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Saccharomyces cerevisiae and Aspergillus niger Rhodotorulla rubra, but it had no effect on these tests. Acute and chronic treatments were performed in order to assess whether the extracts of seeds and the stem bark at concentrations 100 and 250 mg/kg, showed any behavioral changes as in locomotor activity, memory, antidepressant and anxiolytic. The tests were performed on mice, which were submitted to the open field test, inhibitory avoidance, elevated plus maze and forced swimming, respectively. In the open field and memory tests there was no significant change in treatment when compared with controls. The antidepressant effect was demonstrated in acute and chronic treatment of the animals exposed to extracts of the seeds and stem bark of H. courbaril L., at both concentrations. The anxiolytic effect was also confirmed, but only in the chronic treatment of the seeds with the extract at a concentration of 100 mg/kg. |
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Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae)AntidepressivoAntimicrobianoAnsiolíticoAntioxidanteBiscumarinaHymenaea courbaril LCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAAntidepressantAntioxidantAnxiolyticAntimicrobialBiscoumarinsHymenaea courbaril LThe paper presents the chemical and biological study of seed and stem bark of Hymenaea courbaril L. var coubaril. Chemical studies of the seed resulted in the isolation of two biscoumarins: biscoumarin-7-O-β-glucopyranoside-2'-O-α-apiofuranosyl-2'''-O-α-galactopyranoside and 7-O-biscoumarin-β-glucopyranoside-2'-O-α-apiofuranosyl, elucidated by 1H NMR, 13C HMBC, HSQC spectrometry and high resolution mass. The chemical composition of the seeds oil was quantified by GC-MS and it identified the presence of palmitic (11.9%), linoleic (30.6%), arachidic (3.7%) behenic (9.2%) and lignoceric acid (5.8%). With respect to the DPPH• test, the residue of the EtOH extract of the stem bark showed the best antioxidant potential with 97.77%, followed by EtOH extract of the bark (97.71%), EtOH residue of the seeds (96.10%) and AcOEt extract of the seeds (94.90%). The biological study of the oil from the seeds has been done to investigate the antimicrobial activity using standard microorganisms (ATCC strains) with: Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Saccharomyces cerevisiae and Aspergillus niger Rhodotorulla rubra, but it had no effect on these tests. Acute and chronic treatments were performed in order to assess whether the extracts of seeds and the stem bark at concentrations 100 and 250 mg/kg, showed any behavioral changes as in locomotor activity, memory, antidepressant and anxiolytic. The tests were performed on mice, which were submitted to the open field test, inhibitory avoidance, elevated plus maze and forced swimming, respectively. In the open field and memory tests there was no significant change in treatment when compared with controls. The antidepressant effect was demonstrated in acute and chronic treatment of the animals exposed to extracts of the seeds and stem bark of H. courbaril L., at both concentrations. The anxiolytic effect was also confirmed, but only in the chronic treatment of the seeds with the extract at a concentration of 100 mg/kg.O trabalho apresenta o estudo químico e biológico das sementes e cascas do caule de Hymenaea courbaril L. var coubaril. O estudo químico da semente resultou no isolamento de duas biscumarinas: biscumarina-7-O-β-glicopiranosídeo-2’’-O-α-apiofuranosil-2’’’-O-α-galactopiranosil e biscumarina-7-O-β-glicopiranosídeo-2’’-O-α-apiofuranosil, elucidadas por RMN 1H, 13C, HMBC, HSQC e espectrometria de massas de alta resolução. A composição química do óleo das sementes foi determinada por CG-MS que identificou a presença dos ácidos palmítico (11,9%), linoleico (30,6%), araquídico (3,7%), beênico (9,2%) e ácido lignocérico (5,8%). Com relação ao teste de DPPH•, o resíduo do extrato EtOH das cascas do caule apresentou o melhor potencial antioxidante com 97,77%, seguido do extrato EtOH das cascas (97,71%), resíduo EtOH das sementes (96,10%) e extrato AcOEt das sementes (94,90%). O estudo biológico do óleo das sementes foi feito para investigar as atividades antimicrobianas usando microrganismos padrão (cepas ATCC) como: Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Saccharomyces cerevisiae, Rhodotorulla rubra e Aspergillus niger, porém o mesmo não apresentou efeito nesses testes. Foram realizados tratamentos agudo e crônico a fim de avaliar se os extratos das sementes e cascas do caule, nas concentrações 100 e 250 mg/Kg, apresentavam alguma alteração comportamental como na atividade locomotora, memória, antidepressiva e ansiolítica. Os testes foram realizados com camundongos, os quais foram submetidos ao teste do campo aberto, esquiva inibitória, labirinto em cruz elevado e nado forçado, respectivamente. Nos testes de campo aberto e memória não houve nenhuma alteração significativa dos tratamentos quando comparados com os controles. O efeito antidepressivo foi demonstrado no tratamento agudo e crônico dos animais expostos ao extrato das sementes e cascas do caule, em ambas as concentrações. O efeito ansiolítico também foi confirmado, porém somente no tratamento crônico com extrato das sementes na concentração de 100 mg/kg.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Exatas e da Terra (ICET)UFMT CUC - CuiabáPrograma de Pós-Graduação em QuímicaSinhorin, Adilson PauloSinhorin, Valeria Dornelles Gindrihttp://lattes.cnpq.br/3818211604255549http://lattes.cnpq.br/9489541874105935Sinhorin, Adilson Paulo568.067.600-15http://lattes.cnpq.br/9489541874105935Camargo, Maria José de.http://lattes.cnpq.br/1658847665468138568.067.600-15890.945.900-00Silva, Virginia Claudia Paulino.http://lattes.cnpq.br/0840002018932533Fernandes, Hocelayne Paulino2019-11-28T14:34:59Z2015-02-032019-11-28T14:34:59Z2014-10-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisFERNANDES, Hocelayne Paulino. Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae). 2014. 117 f. Dissertação (Mestrado em Química) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2014.http://ri.ufmt.br/handle/1/1625porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2019-12-06T06:03:02Zoai:localhost:1/1625Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2019-12-06T06:03:02Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) |
title |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) |
spellingShingle |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) Fernandes, Hocelayne Paulino Antidepressivo Antimicrobiano Ansiolítico Antioxidante Biscumarina Hymenaea courbaril L CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Antidepressant Antioxidant Anxiolytic Antimicrobial Biscoumarins Hymenaea courbaril L |
title_short |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) |
title_full |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) |
title_fullStr |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) |
title_full_unstemmed |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) |
title_sort |
Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae) |
author |
Fernandes, Hocelayne Paulino |
author_facet |
Fernandes, Hocelayne Paulino |
author_role |
author |
dc.contributor.none.fl_str_mv |
Sinhorin, Adilson Paulo Sinhorin, Valeria Dornelles Gindri http://lattes.cnpq.br/3818211604255549 http://lattes.cnpq.br/9489541874105935 Sinhorin, Adilson Paulo 568.067.600-15 http://lattes.cnpq.br/9489541874105935 Camargo, Maria José de . http://lattes.cnpq.br/1658847665468138 568.067.600-15 890.945.900-00 Silva, Virginia Claudia Paulino . http://lattes.cnpq.br/0840002018932533 |
dc.contributor.author.fl_str_mv |
Fernandes, Hocelayne Paulino |
dc.subject.por.fl_str_mv |
Antidepressivo Antimicrobiano Ansiolítico Antioxidante Biscumarina Hymenaea courbaril L CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Antidepressant Antioxidant Anxiolytic Antimicrobial Biscoumarins Hymenaea courbaril L |
topic |
Antidepressivo Antimicrobiano Ansiolítico Antioxidante Biscumarina Hymenaea courbaril L CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Antidepressant Antioxidant Anxiolytic Antimicrobial Biscoumarins Hymenaea courbaril L |
description |
The paper presents the chemical and biological study of seed and stem bark of Hymenaea courbaril L. var coubaril. Chemical studies of the seed resulted in the isolation of two biscoumarins: biscoumarin-7-O-β-glucopyranoside-2'-O-α-apiofuranosyl-2'''-O-α-galactopyranoside and 7-O-biscoumarin-β-glucopyranoside-2'-O-α-apiofuranosyl, elucidated by 1H NMR, 13C HMBC, HSQC spectrometry and high resolution mass. The chemical composition of the seeds oil was quantified by GC-MS and it identified the presence of palmitic (11.9%), linoleic (30.6%), arachidic (3.7%) behenic (9.2%) and lignoceric acid (5.8%). With respect to the DPPH• test, the residue of the EtOH extract of the stem bark showed the best antioxidant potential with 97.77%, followed by EtOH extract of the bark (97.71%), EtOH residue of the seeds (96.10%) and AcOEt extract of the seeds (94.90%). The biological study of the oil from the seeds has been done to investigate the antimicrobial activity using standard microorganisms (ATCC strains) with: Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Saccharomyces cerevisiae and Aspergillus niger Rhodotorulla rubra, but it had no effect on these tests. Acute and chronic treatments were performed in order to assess whether the extracts of seeds and the stem bark at concentrations 100 and 250 mg/kg, showed any behavioral changes as in locomotor activity, memory, antidepressant and anxiolytic. The tests were performed on mice, which were submitted to the open field test, inhibitory avoidance, elevated plus maze and forced swimming, respectively. In the open field and memory tests there was no significant change in treatment when compared with controls. The antidepressant effect was demonstrated in acute and chronic treatment of the animals exposed to extracts of the seeds and stem bark of H. courbaril L., at both concentrations. The anxiolytic effect was also confirmed, but only in the chronic treatment of the seeds with the extract at a concentration of 100 mg/kg. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-20 2015-02-03 2019-11-28T14:34:59Z 2019-11-28T14:34:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FERNANDES, Hocelayne Paulino. Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae). 2014. 117 f. Dissertação (Mestrado em Química) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2014. http://ri.ufmt.br/handle/1/1625 |
identifier_str_mv |
FERNANDES, Hocelayne Paulino. Estudo fitoquímico e atividade biológica em sementes e cascas do caule de Hymenaea courbaril L. var courbaril (Caesalpinioideae). 2014. 117 f. Dissertação (Mestrado em Química) - Universidade Federal de Mato Grosso, Instituto de Ciências Exatas e da Terra, Cuiabá, 2014. |
url |
http://ri.ufmt.br/handle/1/1625 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Exatas e da Terra (ICET) UFMT CUC - Cuiabá Programa de Pós-Graduação em Química |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Exatas e da Terra (ICET) UFMT CUC - Cuiabá Programa de Pós-Graduação em Química |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
instname_str |
Universidade Federal de Mato Grosso (UFMT) |
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UFMT |
institution |
UFMT |
reponame_str |
Repositório Institucional da UFMT |
collection |
Repositório Institucional da UFMT |
repository.name.fl_str_mv |
Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT) |
repository.mail.fl_str_mv |
jordanbiblio@gmail.com |
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1804648498983862272 |