Papel funcional de fungos endofíticos isolados de Vochysia divergens

Detalhes bibliográficos
Autor(a) principal: Narciso, Esther de Andrade
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMT
Texto Completo: http://ri.ufmt.br/handle/1/1728
Resumo: Populations of Vochysia divergens have expanded in the multiannual flooding period of the Neotropical wetland, being their distribution correlated to the water regime and the acidity of the soil. High concentrations of aluminum can be harmful to the development of seedlings of this specie, that has low resistance to drought. A strategy used by plant species to confer tolerance to stressful environments is the association with endophytic fungi, which are characterized by an asymptomatic colonization of their host. The present study aims to investigate the possible relationship between endophytic fungi and tolerance of V. divergens to stress caused by high concentrations of aluminum and drought. Growth parameters from seedlings submitted to stress were observed in vitro, such as length and biomass of the shoot and root, the number of secondary roots, as well as the activity of antioxidant enzymes. Endophytic fungi isolated from V. divergens’ root were evaluated for mycelial growth rate in culture media enriched with different concentrations of Al just as in medias simulating water restriction. The ability of indoleacetic acid synthesis and phosphate solubilization was also investigated in vitro. Model vegetable species (Sorghum bicolor and Oryza sativa) were used to investigate the potential of endophytic lineages to mitigate water stress. Growth parameters, chlorophyll content and fluorescence rates were measured. The V. divergens seedlings presented growth and development restrictions under stress due to water deficit and high Al concentration exposure. Endophytic strains Phialocephala sp.-v1 and D. phaseolorum-v43 presented tolerance to aluminum, however, they did not promote stress mitigation in V. divergens seedlings. F. oxysporum-v47 and S. cucurbitacearum-v12 strains showed potential in the synthesis of AIA and phosphate solubilization, respectively, both of which presented tolerance to water stress. In general, it can be said that the isolated endophytic strains of V. divergens has not mitigating stress effect in this experimental models evaluated.
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spelling Papel funcional de fungos endofíticos isolados de Vochysia divergensCambaráSecaEstresse oxidativoCNPQ::CIENCIAS BIOLOGICAS::ECOLOGIACambaraDroughtOxidative stressPopulations of Vochysia divergens have expanded in the multiannual flooding period of the Neotropical wetland, being their distribution correlated to the water regime and the acidity of the soil. High concentrations of aluminum can be harmful to the development of seedlings of this specie, that has low resistance to drought. A strategy used by plant species to confer tolerance to stressful environments is the association with endophytic fungi, which are characterized by an asymptomatic colonization of their host. The present study aims to investigate the possible relationship between endophytic fungi and tolerance of V. divergens to stress caused by high concentrations of aluminum and drought. Growth parameters from seedlings submitted to stress were observed in vitro, such as length and biomass of the shoot and root, the number of secondary roots, as well as the activity of antioxidant enzymes. Endophytic fungi isolated from V. divergens’ root were evaluated for mycelial growth rate in culture media enriched with different concentrations of Al just as in medias simulating water restriction. The ability of indoleacetic acid synthesis and phosphate solubilization was also investigated in vitro. Model vegetable species (Sorghum bicolor and Oryza sativa) were used to investigate the potential of endophytic lineages to mitigate water stress. Growth parameters, chlorophyll content and fluorescence rates were measured. The V. divergens seedlings presented growth and development restrictions under stress due to water deficit and high Al concentration exposure. Endophytic strains Phialocephala sp.-v1 and D. phaseolorum-v43 presented tolerance to aluminum, however, they did not promote stress mitigation in V. divergens seedlings. F. oxysporum-v47 and S. cucurbitacearum-v12 strains showed potential in the synthesis of AIA and phosphate solubilization, respectively, both of which presented tolerance to water stress. In general, it can be said that the isolated endophytic strains of V. divergens has not mitigating stress effect in this experimental models evaluated.CAPESPopulações de Vochysia divergens têm se expandido no período plurianual de cheias do Pantanal, sendo sua distribuição correlacionada ao regime hídrico e a acidez do solo. Altas concentrações de alumínio podem ser prejudiciais ao desenvolvimento das plântulas da espécie, que possuem baixa resistência à seca. Uma estratégia utilizada pelas espécies vegetais para conferir tolerância a ambientes estressantes é a associação com fungos endofíticos, que são caracterizados por possuírem uma colonização assintomática de seu hospedeiro. O presente estudo tem como objetivo investigar a possível relação entre fungos endofíticos e a tolerância de V. divergens ao estresse causado pelas altas concentrações de alumínio e pela seca. Foram observados in vitro parâmetros de crescimento das plântulas submetidas ao estresse, tais como comprimento e biomassa da parte aérea quanto da raiz, número de raízes secundárias, como também atividade de enzimas antioxidantes. Fungos endofíticas isoladas da raiz de V. divergens foram avaliados quanto à taxa de crescimento micelial em meios de cultura enriquecidos com diferentes concentrações de Al e em meios simulando restrição hídrica. A capacidade de síntese de ácido indolacético e solubilização de fosfato também foi investigada in vitro. Espécies vegetais modelo (Sorghum bicolor e Oryza sativa) foram utilizadas para investigar o potencial das linhagens endofíticas de mitigar o estresse hídrico. Foram mensurados parâmetros de crescimento, teor de clorofila e taxas de fluorescência. As plântulas de V. divergens apresentaram restrições no crescimento e no desenvolvimento sob estresse por déficit hídrico e pela exposição à alta concentração de Al. Linhagens endofíticas Phialocephala sp.-v1 e D. phaseolorum-v43 apresentaram tolerância à alumínio, entretanto, não promoveram mitigação do estresse em plântulas de V. divergens. As linhagens F. oxysporum-v47 e S. cucurbitacearum-v12 demonstraram potencial na síntese de AIA e na solubilização de fosfato, respectivamente, sendo que ambas apresentaram tolerância ao estresse hídrico. De modo geral, pode-se dizer que as linhagens endofíticas isoladas de V. divergens não tem efeito mitigador do estresse nos modelos experimentais aqui avaliados.Universidade Federal de Mato GrossoBrasilInstituto de Biociências (IB)UFMT CUC - CuiabáPrograma de Pós-Graduação em Ecologia e Conservação da BiodiversidadeSoares, Marcos AntônioCunha, Catia Nunes dahttp://lattes.cnpq.br/3027746551707255http://lattes.cnpq.br/3256599670262972Almeida, Euziclei Gonzaga de569.014.961-68http://lattes.cnpq.br/4183279549863845Brasil, Marivaine da Silva497.019.591-91http://lattes.cnpq.br/6472871804023251034.276.646-51142.861.301-34Campos, Daniela Tiago da Silva823.324.871-15http://lattes.cnpq.br/9325411594216293Ortíz, Carmen Eugenia Rodríguez830.350.526-20http://lattes.cnpq.br/2783222284467182Narciso, Esther de Andrade2019-12-13T16:43:04Z2017-11-102019-12-13T16:43:04Z2016-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisNARCISO, Esther de Andrade. Papel funcional de fungos endofíticos isolados de Vochysia divergens. 2016. 58 f. Dissertação (Mestrado em Ecologia e Conservação da Biodiversidade) - Universidade Federal de Mato Grosso, Instituto de Biociências, Cuiabá, 2016.http://ri.ufmt.br/handle/1/1728porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2019-12-14T06:03:46Zoai:localhost:1/1728Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2019-12-14T06:03:46Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv Papel funcional de fungos endofíticos isolados de Vochysia divergens
title Papel funcional de fungos endofíticos isolados de Vochysia divergens
spellingShingle Papel funcional de fungos endofíticos isolados de Vochysia divergens
Narciso, Esther de Andrade
Cambará
Seca
Estresse oxidativo
CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA
Cambara
Drought
Oxidative stress
title_short Papel funcional de fungos endofíticos isolados de Vochysia divergens
title_full Papel funcional de fungos endofíticos isolados de Vochysia divergens
title_fullStr Papel funcional de fungos endofíticos isolados de Vochysia divergens
title_full_unstemmed Papel funcional de fungos endofíticos isolados de Vochysia divergens
title_sort Papel funcional de fungos endofíticos isolados de Vochysia divergens
author Narciso, Esther de Andrade
author_facet Narciso, Esther de Andrade
author_role author
dc.contributor.none.fl_str_mv Soares, Marcos Antônio
Cunha, Catia Nunes da
http://lattes.cnpq.br/3027746551707255
http://lattes.cnpq.br/3256599670262972
Almeida, Euziclei Gonzaga de
569.014.961-68
http://lattes.cnpq.br/4183279549863845
Brasil, Marivaine da Silva
497.019.591-91
http://lattes.cnpq.br/6472871804023251
034.276.646-51
142.861.301-34
Campos, Daniela Tiago da Silva
823.324.871-15
http://lattes.cnpq.br/9325411594216293
Ortíz, Carmen Eugenia Rodríguez
830.350.526-20
http://lattes.cnpq.br/2783222284467182
dc.contributor.author.fl_str_mv Narciso, Esther de Andrade
dc.subject.por.fl_str_mv Cambará
Seca
Estresse oxidativo
CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA
Cambara
Drought
Oxidative stress
topic Cambará
Seca
Estresse oxidativo
CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA
Cambara
Drought
Oxidative stress
description Populations of Vochysia divergens have expanded in the multiannual flooding period of the Neotropical wetland, being their distribution correlated to the water regime and the acidity of the soil. High concentrations of aluminum can be harmful to the development of seedlings of this specie, that has low resistance to drought. A strategy used by plant species to confer tolerance to stressful environments is the association with endophytic fungi, which are characterized by an asymptomatic colonization of their host. The present study aims to investigate the possible relationship between endophytic fungi and tolerance of V. divergens to stress caused by high concentrations of aluminum and drought. Growth parameters from seedlings submitted to stress were observed in vitro, such as length and biomass of the shoot and root, the number of secondary roots, as well as the activity of antioxidant enzymes. Endophytic fungi isolated from V. divergens’ root were evaluated for mycelial growth rate in culture media enriched with different concentrations of Al just as in medias simulating water restriction. The ability of indoleacetic acid synthesis and phosphate solubilization was also investigated in vitro. Model vegetable species (Sorghum bicolor and Oryza sativa) were used to investigate the potential of endophytic lineages to mitigate water stress. Growth parameters, chlorophyll content and fluorescence rates were measured. The V. divergens seedlings presented growth and development restrictions under stress due to water deficit and high Al concentration exposure. Endophytic strains Phialocephala sp.-v1 and D. phaseolorum-v43 presented tolerance to aluminum, however, they did not promote stress mitigation in V. divergens seedlings. F. oxysporum-v47 and S. cucurbitacearum-v12 strains showed potential in the synthesis of AIA and phosphate solubilization, respectively, both of which presented tolerance to water stress. In general, it can be said that the isolated endophytic strains of V. divergens has not mitigating stress effect in this experimental models evaluated.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-30
2017-11-10
2019-12-13T16:43:04Z
2019-12-13T16:43:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv NARCISO, Esther de Andrade. Papel funcional de fungos endofíticos isolados de Vochysia divergens. 2016. 58 f. Dissertação (Mestrado em Ecologia e Conservação da Biodiversidade) - Universidade Federal de Mato Grosso, Instituto de Biociências, Cuiabá, 2016.
http://ri.ufmt.br/handle/1/1728
identifier_str_mv NARCISO, Esther de Andrade. Papel funcional de fungos endofíticos isolados de Vochysia divergens. 2016. 58 f. Dissertação (Mestrado em Ecologia e Conservação da Biodiversidade) - Universidade Federal de Mato Grosso, Instituto de Biociências, Cuiabá, 2016.
url http://ri.ufmt.br/handle/1/1728
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Biociências (IB)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ecologia e Conservação da Biodiversidade
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Biociências (IB)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ecologia e Conservação da Biodiversidade
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMT
instname:Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Repositório Institucional da UFMT
collection Repositório Institucional da UFMT
repository.name.fl_str_mv Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)
repository.mail.fl_str_mv jordanbiblio@gmail.com
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