ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477 |
Resumo: | Carbon nanotubes (CNT) appear as a new class of materials that have special properties and are the focus of many studies in the biological area. Toxicity studies are important to evaluate the biocompatibility and the possible risks of exposure to the CNT. In this context, we evaluated the reproductive toxicity of exposure to multi-walled carbon nanotubes (MWCNT) in pregnant mice. To this end, pregnant females were exposed orally 100 μl MWCNT/day, suspended in PBS / Tween 80® at a concentration of 5 mg/ml and 15 mg/ml during the implantation period (1st to 6th day of pregnancy), organogenesis (7th to 12th day of pregnancy) or during the fetal period (13th to the 18th day of pregnancy). The females were evaluated for changes in weight and consumption of food and water throughout the experiment. In the 18th day of pregnancy, females were undergoing cesarean section and recorded the number of live fetuses, resorptions and dead. The fetuses were weighed, measured and fixed in 70% alcohol (v/v) for analysis of skeletal changes. Maternal organs, liver, kidney, spleen, heart and lung were collected, weighed and fixed in formaldehyde 10% (v/v) for histological analysis. There were mild signs of maternal toxicity, viewed mainly by histological organs and all embryofetal observed changes occurred in animals exposed during the period of organogenesis. Exposure to MWCNT at a dose of 0.5 mg/day showed an increase in the number of resorptions and reduced number of live fetuses in the control group. The exposure to MWCNT 1.5 mg/day showed an increase in the proportion of resorptions, the average of dead fetuses and a reduction in the proportion of live fetuses, resulting in increased rates of prenatal losses. Changes in body weight of fetuses measures were observed in a dosedependent. Analysis of the skeleton showed a marked delay of ossification, also in a dosedependent. These data support the conclusion that exposure to MWCNT at doses of 0.5 mg/day and 1.5 mg/day, cause an increase in losses and prenatal growth retardation, intrauterine, when administered during organogenesis, and these changes may be related to the direct action of the body MWCNT or fetal abnormalities observed in the morphology of the mother's organs. |
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Hoelzel, Solange Cristina da Silva MartinsFagan, Solange BinottoMonserrat, Laura Alicia GeracitanoNava, Alexandre2018-08-14T19:29:34Z2010-03-30Nava, Alexandre. ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES. 2010. 90f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS .http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477Carbon nanotubes (CNT) appear as a new class of materials that have special properties and are the focus of many studies in the biological area. Toxicity studies are important to evaluate the biocompatibility and the possible risks of exposure to the CNT. In this context, we evaluated the reproductive toxicity of exposure to multi-walled carbon nanotubes (MWCNT) in pregnant mice. To this end, pregnant females were exposed orally 100 μl MWCNT/day, suspended in PBS / Tween 80® at a concentration of 5 mg/ml and 15 mg/ml during the implantation period (1st to 6th day of pregnancy), organogenesis (7th to 12th day of pregnancy) or during the fetal period (13th to the 18th day of pregnancy). The females were evaluated for changes in weight and consumption of food and water throughout the experiment. In the 18th day of pregnancy, females were undergoing cesarean section and recorded the number of live fetuses, resorptions and dead. The fetuses were weighed, measured and fixed in 70% alcohol (v/v) for analysis of skeletal changes. Maternal organs, liver, kidney, spleen, heart and lung were collected, weighed and fixed in formaldehyde 10% (v/v) for histological analysis. There were mild signs of maternal toxicity, viewed mainly by histological organs and all embryofetal observed changes occurred in animals exposed during the period of organogenesis. Exposure to MWCNT at a dose of 0.5 mg/day showed an increase in the number of resorptions and reduced number of live fetuses in the control group. The exposure to MWCNT 1.5 mg/day showed an increase in the proportion of resorptions, the average of dead fetuses and a reduction in the proportion of live fetuses, resulting in increased rates of prenatal losses. Changes in body weight of fetuses measures were observed in a dosedependent. Analysis of the skeleton showed a marked delay of ossification, also in a dosedependent. These data support the conclusion that exposure to MWCNT at doses of 0.5 mg/day and 1.5 mg/day, cause an increase in losses and prenatal growth retardation, intrauterine, when administered during organogenesis, and these changes may be related to the direct action of the body MWCNT or fetal abnormalities observed in the morphology of the mother's organs.Os nanotubos de carbono (NTC) surgem como uma nova classe de materiais que apresentam propriedades especiais e estão sendo foco de muitos estudos na área biológica. Estudos de toxicidade tornam-se importantes para avaliação da biocompatibilidade e dos possíveis riscos da exposição à NTC. Neste contexto, avaliou-se a toxicidade reprodutiva da exposição à nanotubos de carbono de paredes múltiplas (NCPM) em camundongos prenhes. Para tanto, fêmeas grávidas foram expostas oralmente a 100 μL NCPM/dia, suspensos em solução de PBS/Tween 80® na concentração de 5mg/mL e 15 mg/mL, durante o período de implantação (1º ao 6º dia de gestação - ddg), organogênese (7º ao 12º ddg) ou durante o período fetal (13º ao 18º ddg). As fêmeas foram avaliadas quanto a alterações de peso e no consumo de água e ração durante todo o experimento. No 18º ddg, as fêmeas foram submetidas à cesariana sendo registrado o número de fetos vivos, mortos e reabsorções. Os fetos foram pesados, medidos e fixados em álcool 70% (v/v) para análise de alterações esqueléticas. Os órgãos maternos, fígado, rim, baço, coração e pulmão foram coletados, pesados e fixados em formol 10% (v/v) para análise histológica. Observaram-se leves sinais de toxicidade materna, visualizados principalmente por alterações histológicas nos órgãos e todas as alterações embriofetais observadas ocorreram nos animais expostos durante o período da organogênese. A exposição à NCPM, na dose de 0,5 mg/dia mostrou aumento no número de reabsorções e redução no número de fetos vivos em relação ao grupo controle. A exposição a 1,5 mg NCPM/dia mostrou o aumento na proporção de reabsorções, na média de fetos mortos e uma redução na proporção de fetos vivos, culminando no aumento do índice de perdas pré natais. Alterações no peso corporal dos fetos foram observadas de forma dose-dependente. A análise do esqueleto demonstrou um intenso retardo de ossificação, também de forma dose dependente. Esses dados permitem concluir que a exposição à NCPM, nas doses de 0,5 mg/dia e 1,5 mg/dia, causam aumento nas perdas pré-natais e retardo de crescimento intra-uterino, quando administrados durante o período da organogênese e essas alterações podem estar relacionadas com a ação direta dos NCPM no organismo embriofetal ou com as alterações observadas na morfologia dos órgãos maternos.Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2018-08-14T19:29:34Z No. of bitstreams: 2 Dissertacao_AlexandreNava.pdf: 7409778 bytes, checksum: e53af9a26d93c0c8d78123589cb1a973 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-08-14T19:29:34Z (GMT). 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dc.title.por.fl_str_mv |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES |
title |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES |
spellingShingle |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES Nava, Alexandre Nanotubos de Carbono. Toxicidade. Gestação Carbon Nanotube. Toxicity. Pregnancy Biociências e Nanomateriais |
title_short |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES |
title_full |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES |
title_fullStr |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES |
title_full_unstemmed |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES |
title_sort |
ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES |
author |
Nava, Alexandre |
author_facet |
Nava, Alexandre |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Hoelzel, Solange Cristina da Silva Martins |
dc.contributor.referee1.fl_str_mv |
Fagan, Solange Binotto |
dc.contributor.referee2.fl_str_mv |
Monserrat, Laura Alicia Geracitano |
dc.contributor.author.fl_str_mv |
Nava, Alexandre |
contributor_str_mv |
Hoelzel, Solange Cristina da Silva Martins Fagan, Solange Binotto Monserrat, Laura Alicia Geracitano |
dc.subject.por.fl_str_mv |
Nanotubos de Carbono. Toxicidade. Gestação |
topic |
Nanotubos de Carbono. Toxicidade. Gestação Carbon Nanotube. Toxicity. Pregnancy Biociências e Nanomateriais |
dc.subject.eng.fl_str_mv |
Carbon Nanotube. Toxicity. Pregnancy |
dc.subject.cnpq.fl_str_mv |
Biociências e Nanomateriais |
description |
Carbon nanotubes (CNT) appear as a new class of materials that have special properties and are the focus of many studies in the biological area. Toxicity studies are important to evaluate the biocompatibility and the possible risks of exposure to the CNT. In this context, we evaluated the reproductive toxicity of exposure to multi-walled carbon nanotubes (MWCNT) in pregnant mice. To this end, pregnant females were exposed orally 100 μl MWCNT/day, suspended in PBS / Tween 80® at a concentration of 5 mg/ml and 15 mg/ml during the implantation period (1st to 6th day of pregnancy), organogenesis (7th to 12th day of pregnancy) or during the fetal period (13th to the 18th day of pregnancy). The females were evaluated for changes in weight and consumption of food and water throughout the experiment. In the 18th day of pregnancy, females were undergoing cesarean section and recorded the number of live fetuses, resorptions and dead. The fetuses were weighed, measured and fixed in 70% alcohol (v/v) for analysis of skeletal changes. Maternal organs, liver, kidney, spleen, heart and lung were collected, weighed and fixed in formaldehyde 10% (v/v) for histological analysis. There were mild signs of maternal toxicity, viewed mainly by histological organs and all embryofetal observed changes occurred in animals exposed during the period of organogenesis. Exposure to MWCNT at a dose of 0.5 mg/day showed an increase in the number of resorptions and reduced number of live fetuses in the control group. The exposure to MWCNT 1.5 mg/day showed an increase in the proportion of resorptions, the average of dead fetuses and a reduction in the proportion of live fetuses, resulting in increased rates of prenatal losses. Changes in body weight of fetuses measures were observed in a dosedependent. Analysis of the skeleton showed a marked delay of ossification, also in a dosedependent. These data support the conclusion that exposure to MWCNT at doses of 0.5 mg/day and 1.5 mg/day, cause an increase in losses and prenatal growth retardation, intrauterine, when administered during organogenesis, and these changes may be related to the direct action of the body MWCNT or fetal abnormalities observed in the morphology of the mother's organs. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-03-30 |
dc.date.accessioned.fl_str_mv |
2018-08-14T19:29:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Nava, Alexandre. ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES. 2010. 90f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
dc.identifier.uri.fl_str_mv |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477 |
identifier_str_mv |
Nava, Alexandre. ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES. 2010. 90f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
url |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477 |
dc.language.iso.fl_str_mv |
por |
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por |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Centro Universitário Franciscano |
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Programa de Pós-Graduação em Nanociências |
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UNIFRA |
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Brasil |
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Biociências e Nanomateriais |
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Centro Universitário Franciscano |
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