ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES

Detalhes bibliográficos
Autor(a) principal: Nava, Alexandre
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional Universidade Franciscana
Texto Completo: http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477
Resumo: Carbon nanotubes (CNT) appear as a new class of materials that have special properties and are the focus of many studies in the biological area. Toxicity studies are important to evaluate the biocompatibility and the possible risks of exposure to the CNT. In this context, we evaluated the reproductive toxicity of exposure to multi-walled carbon nanotubes (MWCNT) in pregnant mice. To this end, pregnant females were exposed orally 100 μl MWCNT/day, suspended in PBS / Tween 80® at a concentration of 5 mg/ml and 15 mg/ml during the implantation period (1st to 6th day of pregnancy), organogenesis (7th to 12th day of pregnancy) or during the fetal period (13th to the 18th day of pregnancy). The females were evaluated for changes in weight and consumption of food and water throughout the experiment. In the 18th day of pregnancy, females were undergoing cesarean section and recorded the number of live fetuses, resorptions and dead. The fetuses were weighed, measured and fixed in 70% alcohol (v/v) for analysis of skeletal changes. Maternal organs, liver, kidney, spleen, heart and lung were collected, weighed and fixed in formaldehyde 10% (v/v) for histological analysis. There were mild signs of maternal toxicity, viewed mainly by histological organs and all embryofetal observed changes occurred in animals exposed during the period of organogenesis. Exposure to MWCNT at a dose of 0.5 mg/day showed an increase in the number of resorptions and reduced number of live fetuses in the control group. The exposure to MWCNT 1.5 mg/day showed an increase in the proportion of resorptions, the average of dead fetuses and a reduction in the proportion of live fetuses, resulting in increased rates of prenatal losses. Changes in body weight of fetuses measures were observed in a dosedependent. Analysis of the skeleton showed a marked delay of ossification, also in a dosedependent. These data support the conclusion that exposure to MWCNT at doses of 0.5 mg/day and 1.5 mg/day, cause an increase in losses and prenatal growth retardation, intrauterine, when administered during organogenesis, and these changes may be related to the direct action of the body MWCNT or fetal abnormalities observed in the morphology of the mother's organs.
id UFN-1_119632d18b32c59139d8dcd3b07628c7
oai_identifier_str oai:tede.universidadefranciscana.edu.br:UFN-BDTD/477
network_acronym_str UFN-1
network_name_str Repositório Institucional Universidade Franciscana
repository_id_str http://www.tede.universidadefranciscana.edu.br:8080/oai/request
spelling Hoelzel, Solange Cristina da Silva MartinsFagan, Solange BinottoMonserrat, Laura Alicia GeracitanoNava, Alexandre2018-08-14T19:29:34Z2010-03-30Nava, Alexandre. ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES. 2010. 90f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS .http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477Carbon nanotubes (CNT) appear as a new class of materials that have special properties and are the focus of many studies in the biological area. Toxicity studies are important to evaluate the biocompatibility and the possible risks of exposure to the CNT. In this context, we evaluated the reproductive toxicity of exposure to multi-walled carbon nanotubes (MWCNT) in pregnant mice. To this end, pregnant females were exposed orally 100 μl MWCNT/day, suspended in PBS / Tween 80® at a concentration of 5 mg/ml and 15 mg/ml during the implantation period (1st to 6th day of pregnancy), organogenesis (7th to 12th day of pregnancy) or during the fetal period (13th to the 18th day of pregnancy). The females were evaluated for changes in weight and consumption of food and water throughout the experiment. In the 18th day of pregnancy, females were undergoing cesarean section and recorded the number of live fetuses, resorptions and dead. The fetuses were weighed, measured and fixed in 70% alcohol (v/v) for analysis of skeletal changes. Maternal organs, liver, kidney, spleen, heart and lung were collected, weighed and fixed in formaldehyde 10% (v/v) for histological analysis. There were mild signs of maternal toxicity, viewed mainly by histological organs and all embryofetal observed changes occurred in animals exposed during the period of organogenesis. Exposure to MWCNT at a dose of 0.5 mg/day showed an increase in the number of resorptions and reduced number of live fetuses in the control group. The exposure to MWCNT 1.5 mg/day showed an increase in the proportion of resorptions, the average of dead fetuses and a reduction in the proportion of live fetuses, resulting in increased rates of prenatal losses. Changes in body weight of fetuses measures were observed in a dosedependent. Analysis of the skeleton showed a marked delay of ossification, also in a dosedependent. These data support the conclusion that exposure to MWCNT at doses of 0.5 mg/day and 1.5 mg/day, cause an increase in losses and prenatal growth retardation, intrauterine, when administered during organogenesis, and these changes may be related to the direct action of the body MWCNT or fetal abnormalities observed in the morphology of the mother's organs.Os nanotubos de carbono (NTC) surgem como uma nova classe de materiais que apresentam propriedades especiais e estão sendo foco de muitos estudos na área biológica. Estudos de toxicidade tornam-se importantes para avaliação da biocompatibilidade e dos possíveis riscos da exposição à NTC. Neste contexto, avaliou-se a toxicidade reprodutiva da exposição à nanotubos de carbono de paredes múltiplas (NCPM) em camundongos prenhes. Para tanto, fêmeas grávidas foram expostas oralmente a 100 μL NCPM/dia, suspensos em solução de PBS/Tween 80® na concentração de 5mg/mL e 15 mg/mL, durante o período de implantação (1º ao 6º dia de gestação - ddg), organogênese (7º ao 12º ddg) ou durante o período fetal (13º ao 18º ddg). As fêmeas foram avaliadas quanto a alterações de peso e no consumo de água e ração durante todo o experimento. No 18º ddg, as fêmeas foram submetidas à cesariana sendo registrado o número de fetos vivos, mortos e reabsorções. Os fetos foram pesados, medidos e fixados em álcool 70% (v/v) para análise de alterações esqueléticas. Os órgãos maternos, fígado, rim, baço, coração e pulmão foram coletados, pesados e fixados em formol 10% (v/v) para análise histológica. Observaram-se leves sinais de toxicidade materna, visualizados principalmente por alterações histológicas nos órgãos e todas as alterações embriofetais observadas ocorreram nos animais expostos durante o período da organogênese. A exposição à NCPM, na dose de 0,5 mg/dia mostrou aumento no número de reabsorções e redução no número de fetos vivos em relação ao grupo controle. A exposição a 1,5 mg NCPM/dia mostrou o aumento na proporção de reabsorções, na média de fetos mortos e uma redução na proporção de fetos vivos, culminando no aumento do índice de perdas pré natais. Alterações no peso corporal dos fetos foram observadas de forma dose-dependente. A análise do esqueleto demonstrou um intenso retardo de ossificação, também de forma dose dependente. Esses dados permitem concluir que a exposição à NCPM, nas doses de 0,5 mg/dia e 1,5 mg/dia, causam aumento nas perdas pré-natais e retardo de crescimento intra-uterino, quando administrados durante o período da organogênese e essas alterações podem estar relacionadas com a ação direta dos NCPM no organismo embriofetal ou com as alterações observadas na morfologia dos órgãos maternos.Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2018-08-14T19:29:34Z No. of bitstreams: 2 Dissertacao_AlexandreNava.pdf: 7409778 bytes, checksum: e53af9a26d93c0c8d78123589cb1a973 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-08-14T19:29:34Z (GMT). No. of bitstreams: 2 Dissertacao_AlexandreNava.pdf: 7409778 bytes, checksum: e53af9a26d93c0c8d78123589cb1a973 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2010-03-30application/pdfhttp://www.tede.universidadefranciscana.edu.br:8080/retrieve/1374/Dissertacao_AlexandreNava.pdf.jpgporCentro Universitário FranciscanoPrograma de Pós-Graduação em NanociênciasUNIFRABrasilBiociências e Nanomateriaishttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessNanotubos de Carbono. Toxicidade. GestaçãoCarbon Nanotube. Toxicity. PregnancyBiociências e NanomateriaisANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHESinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Repositório Institucional Universidade Franciscanainstname:Universidade Franciscana (UFN)instacron:UFNORIGINALDissertacao_AlexandreNava.pdfDissertacao_AlexandreNava.pdfapplication/pdf7409778http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/5/Dissertacao_AlexandreNava.pdfe53af9a26d93c0c8d78123589cb1a973MD55TEXTDissertacao_AlexandreNava.pdf.txtDissertacao_AlexandreNava.pdf.txttext/plain147318http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/6/Dissertacao_AlexandreNava.pdf.txt2efcb934aeddafd9aa0074818849ced7MD56THUMBNAILDissertacao_AlexandreNava.pdf.jpgDissertacao_AlexandreNava.pdf.jpgimage/jpeg3394http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/7/Dissertacao_AlexandreNava.pdf.jpg0468f8b34b379928f05335b2f501d0b1MD57LICENSElicense.txtlicense.txttext/plain; charset=utf-8309http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/1/license.txte4ae80c7384074d77a55dabcdffdf13aMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54UFN-BDTD/4772018-08-16 01:00:39.41oai:tede.universidadefranciscana.edu.br:UFN-BDTD/477RXN0ZSB0cmFiYWxobyBzZXLDoSBsaWNlbmNpYWRvIHNvYiBhIExpY2Vuw6dhIEF0cmlidWnDp8Ojby1Ow6NvQ29tZXJjaWFsLVNlbURlcml2YcOnw7VlcyA0LjAgSW50ZXJuYWNpb25hbCBDcmVhdGl2ZSBDb21tb25zLiBQYXJhIHZpc3VhbGl6YXIgdW1hIGPDs3BpYSBkZXN0YSBsaWNlbsOnYSwgdmlzaXRlIGh0dHA6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LW5jLW5kLzQuMC8gb3UgbWFuZGUgdW1hIGNhcnRhIHBhcmEgQ3JlYXRpdmUgQ29tbW9ucywgUE8gQm94IDE4NjYsIE1vdW50YWluIFZpZXcsIENBIDk0MDQyLCBVU0EuRepositório de Publicaçõeshttp://www.tede.universidadefranciscana.edu.br:8080/http://www.tede.universidadefranciscana.edu.br:8080/oai/requestopendoar:2018-08-16T04:00:39Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)false
dc.title.por.fl_str_mv ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
title ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
spellingShingle ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
Nava, Alexandre
Nanotubos de Carbono. Toxicidade. Gestação
Carbon Nanotube. Toxicity. Pregnancy
Biociências e Nanomateriais
title_short ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
title_full ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
title_fullStr ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
title_full_unstemmed ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
title_sort ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES
author Nava, Alexandre
author_facet Nava, Alexandre
author_role author
dc.contributor.advisor1.fl_str_mv Hoelzel, Solange Cristina da Silva Martins
dc.contributor.referee1.fl_str_mv Fagan, Solange Binotto
dc.contributor.referee2.fl_str_mv Monserrat, Laura Alicia Geracitano
dc.contributor.author.fl_str_mv Nava, Alexandre
contributor_str_mv Hoelzel, Solange Cristina da Silva Martins
Fagan, Solange Binotto
Monserrat, Laura Alicia Geracitano
dc.subject.por.fl_str_mv Nanotubos de Carbono. Toxicidade. Gestação
topic Nanotubos de Carbono. Toxicidade. Gestação
Carbon Nanotube. Toxicity. Pregnancy
Biociências e Nanomateriais
dc.subject.eng.fl_str_mv Carbon Nanotube. Toxicity. Pregnancy
dc.subject.cnpq.fl_str_mv Biociências e Nanomateriais
description Carbon nanotubes (CNT) appear as a new class of materials that have special properties and are the focus of many studies in the biological area. Toxicity studies are important to evaluate the biocompatibility and the possible risks of exposure to the CNT. In this context, we evaluated the reproductive toxicity of exposure to multi-walled carbon nanotubes (MWCNT) in pregnant mice. To this end, pregnant females were exposed orally 100 μl MWCNT/day, suspended in PBS / Tween 80® at a concentration of 5 mg/ml and 15 mg/ml during the implantation period (1st to 6th day of pregnancy), organogenesis (7th to 12th day of pregnancy) or during the fetal period (13th to the 18th day of pregnancy). The females were evaluated for changes in weight and consumption of food and water throughout the experiment. In the 18th day of pregnancy, females were undergoing cesarean section and recorded the number of live fetuses, resorptions and dead. The fetuses were weighed, measured and fixed in 70% alcohol (v/v) for analysis of skeletal changes. Maternal organs, liver, kidney, spleen, heart and lung were collected, weighed and fixed in formaldehyde 10% (v/v) for histological analysis. There were mild signs of maternal toxicity, viewed mainly by histological organs and all embryofetal observed changes occurred in animals exposed during the period of organogenesis. Exposure to MWCNT at a dose of 0.5 mg/day showed an increase in the number of resorptions and reduced number of live fetuses in the control group. The exposure to MWCNT 1.5 mg/day showed an increase in the proportion of resorptions, the average of dead fetuses and a reduction in the proportion of live fetuses, resulting in increased rates of prenatal losses. Changes in body weight of fetuses measures were observed in a dosedependent. Analysis of the skeleton showed a marked delay of ossification, also in a dosedependent. These data support the conclusion that exposure to MWCNT at doses of 0.5 mg/day and 1.5 mg/day, cause an increase in losses and prenatal growth retardation, intrauterine, when administered during organogenesis, and these changes may be related to the direct action of the body MWCNT or fetal abnormalities observed in the morphology of the mother's organs.
publishDate 2010
dc.date.issued.fl_str_mv 2010-03-30
dc.date.accessioned.fl_str_mv 2018-08-14T19:29:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Nava, Alexandre. ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES. 2010. 90f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS .
dc.identifier.uri.fl_str_mv http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477
identifier_str_mv Nava, Alexandre. ANÁLISE TOXICOLÓGICA DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM CAMUNDONGOS PRENHES. 2010. 90f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS .
url http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/477
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Centro Universitário Franciscano
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Nanociências
dc.publisher.initials.fl_str_mv UNIFRA
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Biociências e Nanomateriais
publisher.none.fl_str_mv Centro Universitário Franciscano
dc.source.none.fl_str_mv reponame:Repositório Institucional Universidade Franciscana
instname:Universidade Franciscana (UFN)
instacron:UFN
instname_str Universidade Franciscana (UFN)
instacron_str UFN
institution UFN
reponame_str Repositório Institucional Universidade Franciscana
collection Repositório Institucional Universidade Franciscana
bitstream.url.fl_str_mv http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/5/Dissertacao_AlexandreNava.pdf
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/6/Dissertacao_AlexandreNava.pdf.txt
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/7/Dissertacao_AlexandreNava.pdf.jpg
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/1/license.txt
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/2/license_url
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/3/license_text
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/477/4/license_rdf
bitstream.checksum.fl_str_mv e53af9a26d93c0c8d78123589cb1a973
2efcb934aeddafd9aa0074818849ced7
0468f8b34b379928f05335b2f501d0b1
e4ae80c7384074d77a55dabcdffdf13a
4afdbb8c545fd630ea7db775da747b2f
d41d8cd98f00b204e9800998ecf8427e
d41d8cd98f00b204e9800998ecf8427e
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)
repository.mail.fl_str_mv
_version_ 1809269398648651776