AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/791 |
Resumo: | Alternative treatments with the use of natural agents are highlighted. In addition, intelligent delivery systems such as nanostructured systems are also growing. Therefore the proposal of this work was to develop a nanoemulsion containing Tucumã oil, to characterize it and to analyze its pharmacological activity in vitro and in vivo. Considering the beneficial properties of Tucumã, the objective was to use it in the form of nanoemulsion, in order to protect the photosensitivity presented by the oil, as well as to develop a more efficient system for the delivery of those favorable activities provided by its compounds. First, a preformulation study of the nanoemulsion containing Tucuman oil was carried out, together with the analysis of different concentrations of these formulations. After these results demonstrated a safety in the concentration of 500 μg / mL in emulsions with the lowest oil content, we decided to work only with the formulation that comprised the smallest quantity. After preparation of the formulations they were characterized by proposing a shelf life. The following parameters were analyzed: refractive index, mean particle diameter, polydispersity index, zeta potential, pH and microscopy on days 0, 7, 15, 30, 60 and 90 days at different temperatures: cooling, climatic chamber and room temperature. Through the results of this step it is possible to highlight the refrigeration condition as the best form of storage, showing stability in the mean particle size of 169.1 nm, polydispersion index of 0.11 zeta potential of - 18.3 mV and pH of 4 , 6 mV at the end of 90 days. In the in vivo assays we have highlighted alternative models such as fish to examine possible toxic effects in experiments with molecules and nanoparticles in different routes of administration as performed in this study. In this way it was possible to demonstrate a toxic synergism between surfactants used, causing different lesions and behavior changes in the animals, as well as possible aggregations formed from these nanostructures. At the same time, the concentrations of TSO 100, NE 100 and 300 and NEBr 300 were those that were shown to be more toxic, leading to the totality of death at the end of the 7 days of oral treatment. It is clear that toxicity tests of nanoformulations are extremely important for clarification in the biological system of living beings and the environment. |
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Sagrillo, Michele RoratoMachado , Alencar KolinskiZeppenfeldt, Carla CristinaSouza, Diego dePintos, Francieli Guedes2019-08-26T21:01:46Z2019-03-30Pintos, Francieli Guedes. AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO. 2019. 71f. Dissertação( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS .http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/791Alternative treatments with the use of natural agents are highlighted. In addition, intelligent delivery systems such as nanostructured systems are also growing. Therefore the proposal of this work was to develop a nanoemulsion containing Tucumã oil, to characterize it and to analyze its pharmacological activity in vitro and in vivo. Considering the beneficial properties of Tucumã, the objective was to use it in the form of nanoemulsion, in order to protect the photosensitivity presented by the oil, as well as to develop a more efficient system for the delivery of those favorable activities provided by its compounds. First, a preformulation study of the nanoemulsion containing Tucuman oil was carried out, together with the analysis of different concentrations of these formulations. After these results demonstrated a safety in the concentration of 500 μg / mL in emulsions with the lowest oil content, we decided to work only with the formulation that comprised the smallest quantity. After preparation of the formulations they were characterized by proposing a shelf life. The following parameters were analyzed: refractive index, mean particle diameter, polydispersity index, zeta potential, pH and microscopy on days 0, 7, 15, 30, 60 and 90 days at different temperatures: cooling, climatic chamber and room temperature. Through the results of this step it is possible to highlight the refrigeration condition as the best form of storage, showing stability in the mean particle size of 169.1 nm, polydispersion index of 0.11 zeta potential of - 18.3 mV and pH of 4 , 6 mV at the end of 90 days. In the in vivo assays we have highlighted alternative models such as fish to examine possible toxic effects in experiments with molecules and nanoparticles in different routes of administration as performed in this study. In this way it was possible to demonstrate a toxic synergism between surfactants used, causing different lesions and behavior changes in the animals, as well as possible aggregations formed from these nanostructures. At the same time, the concentrations of TSO 100, NE 100 and 300 and NEBr 300 were those that were shown to be more toxic, leading to the totality of death at the end of the 7 days of oral treatment. It is clear that toxicity tests of nanoformulations are extremely important for clarification in the biological system of living beings and the environment.Tratamentos alternativos com uso de agentes naturais ganham destaque. Além disso sistemas de entregas inteligentes, como sistemas nanoestruturados também encontram-se em crescimento. Sendo assim a proposta deste trabalho foi desenvolver uma nanoemulsão contendo óleo de Tucumã, caracterizá-la e analisar sua atividade farmacológica in vitro e in vivo. Considerando as propriedades benéficas do Tucumã, o objetivo foi utilizá-lo na forma de nanoemulsão, a fim de proteger a fotossensibilidade apresentada pelo óleo, assim como desenvolver um sistema mais eficiente para a entrega dessas atividades favoráveis fornecidas pelos seus compostos. Primeiramente foi realizado um estudo de pré-formulação da nanoemulsão contendo óleo de Tucumã, juntamente com a análise de diferentes concentrações destas formulações. Após estes resultados demonstrarem uma segurança na concentração de 500 μg/mL em emulsões com o menor teor de óleo, decidimos trabalhar somente com a formulação que compreendia a menor quantidade. Após a preparação das formulações elas foram caracterizadas propondo uma vida de prateleira. Sendo analisados os seguintes parâmetros: índice de refração, diâmetro médio de partícula, índice de polidispersão, potencial zeta, pH e microscopia nos dias 0, 7, 15, 30, 60 e 90 dias em diferentes temperaturas: refrigeração, câmara climática e temperatura ambiente. Através dos resultados desta etapa é possível destacar a condição de refrigeração como melhor forma de armazenagem, demonstrando estabilidade no tamanho médio de partícula de 169,1 nm, índice de polidispersão de 0,11 potencial zeta de – 18,3 mV e pH de 4,6 mV ao fim dos 90 dias. Nos ensaios in vivo destacamos modelos alternativos como os peixes para examinar possíveis efeitos tóxicos em experimentos com moléculas e nanopartículas em diferentes vias de administração, como realizado neste estudo. Deste modo foi possível demonstrar um provável sinergismo tóxico entre tensoativos utilizados, ocasionando diferentes lesões e mudanças de comportamento nos animais, assim como possíveis agregações formadas a partir destas nanoestruturas. Paralelamente a isto as concentrações de TSO 100, NE 100 e 300 e NEBr 300 foram às que se demonstraram mais toxicidade levando a totalidade de indivíduos a morte ao fim dos 7 dias de tratamento por via oral. Ficando claro que testes de toxicidade de nanoformulações são extremamente importantes para esclarecimentos no sistema biológico de seres vivos e meio ambiente.Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2019-08-26T21:01:46Z No. of bitstreams: 2 Dissertacao_FrancieliGuedesPintos.pdf: 2163935 bytes, checksum: 38e8e4c6a726e2a2a0f4586a2ddb7192 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-08-26T21:01:46Z (GMT). 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dc.title.por.fl_str_mv |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO |
title |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO |
spellingShingle |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO Pintos, Francieli Guedes Nanotoxicologia, modelo animal alternativo, Tucumã, Nanoemulsão, peixes Nanotoxicology, alternative animal model, Tucumã Nanoemulsion, fish Nanociências |
title_short |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO |
title_full |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO |
title_fullStr |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO |
title_full_unstemmed |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO |
title_sort |
AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO |
author |
Pintos, Francieli Guedes |
author_facet |
Pintos, Francieli Guedes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Sagrillo, Michele Rorato |
dc.contributor.advisor-co1.fl_str_mv |
Machado , Alencar Kolinski |
dc.contributor.referee1.fl_str_mv |
Zeppenfeldt, Carla Cristina |
dc.contributor.referee2.fl_str_mv |
Souza, Diego de |
dc.contributor.author.fl_str_mv |
Pintos, Francieli Guedes |
contributor_str_mv |
Sagrillo, Michele Rorato Machado , Alencar Kolinski Zeppenfeldt, Carla Cristina Souza, Diego de |
dc.subject.por.fl_str_mv |
Nanotoxicologia, modelo animal alternativo, Tucumã, Nanoemulsão, peixes |
topic |
Nanotoxicologia, modelo animal alternativo, Tucumã, Nanoemulsão, peixes Nanotoxicology, alternative animal model, Tucumã Nanoemulsion, fish Nanociências |
dc.subject.eng.fl_str_mv |
Nanotoxicology, alternative animal model, Tucumã Nanoemulsion, fish |
dc.subject.cnpq.fl_str_mv |
Nanociências |
description |
Alternative treatments with the use of natural agents are highlighted. In addition, intelligent delivery systems such as nanostructured systems are also growing. Therefore the proposal of this work was to develop a nanoemulsion containing Tucumã oil, to characterize it and to analyze its pharmacological activity in vitro and in vivo. Considering the beneficial properties of Tucumã, the objective was to use it in the form of nanoemulsion, in order to protect the photosensitivity presented by the oil, as well as to develop a more efficient system for the delivery of those favorable activities provided by its compounds. First, a preformulation study of the nanoemulsion containing Tucuman oil was carried out, together with the analysis of different concentrations of these formulations. After these results demonstrated a safety in the concentration of 500 μg / mL in emulsions with the lowest oil content, we decided to work only with the formulation that comprised the smallest quantity. After preparation of the formulations they were characterized by proposing a shelf life. The following parameters were analyzed: refractive index, mean particle diameter, polydispersity index, zeta potential, pH and microscopy on days 0, 7, 15, 30, 60 and 90 days at different temperatures: cooling, climatic chamber and room temperature. Through the results of this step it is possible to highlight the refrigeration condition as the best form of storage, showing stability in the mean particle size of 169.1 nm, polydispersion index of 0.11 zeta potential of - 18.3 mV and pH of 4 , 6 mV at the end of 90 days. In the in vivo assays we have highlighted alternative models such as fish to examine possible toxic effects in experiments with molecules and nanoparticles in different routes of administration as performed in this study. In this way it was possible to demonstrate a toxic synergism between surfactants used, causing different lesions and behavior changes in the animals, as well as possible aggregations formed from these nanostructures. At the same time, the concentrations of TSO 100, NE 100 and 300 and NEBr 300 were those that were shown to be more toxic, leading to the totality of death at the end of the 7 days of oral treatment. It is clear that toxicity tests of nanoformulations are extremely important for clarification in the biological system of living beings and the environment. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-08-26T21:01:46Z |
dc.date.issued.fl_str_mv |
2019-03-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Pintos, Francieli Guedes. AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO. 2019. 71f. Dissertação( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS . |
dc.identifier.uri.fl_str_mv |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/791 |
identifier_str_mv |
Pintos, Francieli Guedes. AVALIAÇÃO DAS PROPRIEDADES NANOTOXICOLÓGICAS DO ÓLEO DO GÊNERO Astrocaryum NAS FORMAS LIVRE E NANOEMULSIONADA IN VIVO. 2019. 71f. Dissertação( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS . |
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http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/791 |
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por |
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por |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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openAccess |
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Programa de Pós-Graduação em Nanociências |
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UFN |
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Brasil |
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Biociências e Nanomateriais |
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Universidade Franciscana |
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