Cell growth on 3D microstructured surfaces.

• Autor(a) principal: Araujo, Wagner Wlysses Rodrigues de
• Data de Publicação: 2016
• Outros Autores: Teixeira, Fernanda de Sá, Silva, Glenda Nicioli da, Salvadori, Daisy Maria Fávero, Salvadori, Maria Cecília Barbosa da Silveira, Brown, I. G.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/handle/123456789/6587; https://doi.org/10.1016/j.msec.2016.03.026
• topic: Biomaterials; Surface patterning; Cell aggregation; Optical microscopy

Chinese Hamster Ovary (CHO) cell cultures were grown on surfaces lithographed with periodic 3D hexagonal microcavity array morphology. The range of microcavity size (inscribed circle diameter) was from 12 μm to 560 μm. CHO cells were grown also on flat surfaces. The characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence opticalmicroscopy and evaluated by correlation function analysis. We found that optimum microcavity radius was 80 μm, concerning to the maximum cell growth density, being even greater than the growth density on a flat (unstructured) substrate of the same material. This finding can be important for optimization of biotechnological processes and devices.