Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.

Detalhes bibliográficos
Autor(a) principal: Souza, Daniel Menezes
Data de Publicação: 2011
Outros Autores: Oliveira, Rodrigo Corrêa de, Cota, Renata Guerra de Sá, Giunchetti, Rodolfo Cordeiro, Carvalho, Andréa Teixeira de, Martins Filho, Olindo Assis, Oliveira, Guilherme Corrêa de, Reis, Alexandre Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/892
Resumo: The immune response in the skin of dogs infected withLeishmania chagasi and its asso-ciation with distinct levels of tissue parasitism and clinical progression of canine visceral leishmaniasis (CVL) are poorly understood and limited studies are available. A detailed analysis of the profiles of cytokines (IFN- , IL-4, IL-5, IL-10, IL-12, IL-13, TGF- 1 and TNF- ) and transcription factors (T-bet, GATA-3 and FOXP3) in the skin of 35 naturally infected dogs was carried out using real-time PCR alongside determinations of skin parasite density and the clinical status of CVL. A mixed cytokine profile with high levels of expression of IFN- , TNF- and IL-13 was determined in asymptomatic dogs. Additionally, the levels of transcription factors GATA-3 and FOXP3 were correlated with the asymptomatic disease. A mixed cytokine profile was also observed during active CVL. Moreover, high levels of IL-10 and TGF- 1, concomitant with the low expression of IL-12, may represent a key condition that allows persistence of parasite replication in the skin. The results obtained indicate that in asymptomatic disease or lower levels of skin parasite density, a mixed inflammatory, regulatory immune response profile may be of major relevance for both the maintenance of the clinical status of the dogs as well as for parasite persistence and replication at low levels.
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spelling Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.Canine visceral leishmaniasisCytokinesTranscription factorLeishmania chagasiParasitismThe immune response in the skin of dogs infected withLeishmania chagasi and its asso-ciation with distinct levels of tissue parasitism and clinical progression of canine visceral leishmaniasis (CVL) are poorly understood and limited studies are available. A detailed analysis of the profiles of cytokines (IFN- , IL-4, IL-5, IL-10, IL-12, IL-13, TGF- 1 and TNF- ) and transcription factors (T-bet, GATA-3 and FOXP3) in the skin of 35 naturally infected dogs was carried out using real-time PCR alongside determinations of skin parasite density and the clinical status of CVL. A mixed cytokine profile with high levels of expression of IFN- , TNF- and IL-13 was determined in asymptomatic dogs. Additionally, the levels of transcription factors GATA-3 and FOXP3 were correlated with the asymptomatic disease. A mixed cytokine profile was also observed during active CVL. Moreover, high levels of IL-10 and TGF- 1, concomitant with the low expression of IL-12, may represent a key condition that allows persistence of parasite replication in the skin. The results obtained indicate that in asymptomatic disease or lower levels of skin parasite density, a mixed inflammatory, regulatory immune response profile may be of major relevance for both the maintenance of the clinical status of the dogs as well as for parasite persistence and replication at low levels.2012-06-19T16:41:03Z2012-06-19T16:41:03Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSOUZA, D. M. et al. Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status. Veterinary Parasitology, v.177, p. 39-49, 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S030440171000662X>. Acesso em: 18 jun. 2012.03044017http://www.repositorio.ufop.br/handle/123456789/892O Periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3287140946746.info:eu-repo/semantics/openAccessSouza, Daniel MenezesOliveira, Rodrigo Corrêa deCota, Renata Guerra de SáGiunchetti, Rodolfo CordeiroCarvalho, Andréa Teixeira deMartins Filho, Olindo AssisOliveira, Guilherme Corrêa deReis, Alexandre Barbosaengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-02-22T19:32:07Zoai:repositorio.ufop.br:123456789/892Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-02-22T19:32:07Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
title Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
spellingShingle Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
Souza, Daniel Menezes
Canine visceral leishmaniasis
Cytokines
Transcription factor
Leishmania chagasi
Parasitism
title_short Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
title_full Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
title_fullStr Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
title_full_unstemmed Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
title_sort Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.
author Souza, Daniel Menezes
author_facet Souza, Daniel Menezes
Oliveira, Rodrigo Corrêa de
Cota, Renata Guerra de Sá
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Oliveira, Guilherme Corrêa de
Reis, Alexandre Barbosa
author_role author
author2 Oliveira, Rodrigo Corrêa de
Cota, Renata Guerra de Sá
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Oliveira, Guilherme Corrêa de
Reis, Alexandre Barbosa
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Daniel Menezes
Oliveira, Rodrigo Corrêa de
Cota, Renata Guerra de Sá
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Oliveira, Guilherme Corrêa de
Reis, Alexandre Barbosa
dc.subject.por.fl_str_mv Canine visceral leishmaniasis
Cytokines
Transcription factor
Leishmania chagasi
Parasitism
topic Canine visceral leishmaniasis
Cytokines
Transcription factor
Leishmania chagasi
Parasitism
description The immune response in the skin of dogs infected withLeishmania chagasi and its asso-ciation with distinct levels of tissue parasitism and clinical progression of canine visceral leishmaniasis (CVL) are poorly understood and limited studies are available. A detailed analysis of the profiles of cytokines (IFN- , IL-4, IL-5, IL-10, IL-12, IL-13, TGF- 1 and TNF- ) and transcription factors (T-bet, GATA-3 and FOXP3) in the skin of 35 naturally infected dogs was carried out using real-time PCR alongside determinations of skin parasite density and the clinical status of CVL. A mixed cytokine profile with high levels of expression of IFN- , TNF- and IL-13 was determined in asymptomatic dogs. Additionally, the levels of transcription factors GATA-3 and FOXP3 were correlated with the asymptomatic disease. A mixed cytokine profile was also observed during active CVL. Moreover, high levels of IL-10 and TGF- 1, concomitant with the low expression of IL-12, may represent a key condition that allows persistence of parasite replication in the skin. The results obtained indicate that in asymptomatic disease or lower levels of skin parasite density, a mixed inflammatory, regulatory immune response profile may be of major relevance for both the maintenance of the clinical status of the dogs as well as for parasite persistence and replication at low levels.
publishDate 2011
dc.date.none.fl_str_mv 2011
2012-06-19T16:41:03Z
2012-06-19T16:41:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUZA, D. M. et al. Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status. Veterinary Parasitology, v.177, p. 39-49, 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S030440171000662X>. Acesso em: 18 jun. 2012.
03044017
http://www.repositorio.ufop.br/handle/123456789/892
identifier_str_mv SOUZA, D. M. et al. Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status. Veterinary Parasitology, v.177, p. 39-49, 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S030440171000662X>. Acesso em: 18 jun. 2012.
03044017
url http://www.repositorio.ufop.br/handle/123456789/892
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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