Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi

Detalhes bibliográficos
Autor(a) principal: Almeida, Maria Angela Ornelas de
Data de Publicação: 2005
Outros Autores: Jesus, E. E. V., Atta, Maria Luiza Brito de Sousa, Alves, L. C., Berne, M. E. A., Atta, Ajax Mercês
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6444
Resumo: Trabalho completo: acesso restrito, p.151–158
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spelling Almeida, Maria Angela Ornelas deJesus, E. E. V.Atta, Maria Luiza Brito de SousaAlves, L. C.Berne, M. E. A.Atta, Ajax MercêsAlmeida, Maria Angela Ornelas deJesus, E. E. V.Atta, Maria Luiza Brito de SousaAlves, L. C.Berne, M. E. A.Atta, Ajax Mercês2012-07-17T15:40:05Z2005-06-150165-2427http://www.repositorio.ufba.br/ri/handle/ri/6444v. 106, n. 1–2Trabalho completo: acesso restrito, p.151–158Visceral leishmaniasis (VL) presents vigorous Th2 immune response, which is mainly characterized in human by augmented expression of Il-4, polyclonal B cell activation, intense hypergammaglobulinemia and production of antileishmanial IgE antibodies. However, few aspects of this type of immune response have been demonstrated in studies of canine visceral leishmaniasis (CVL). This work investigated by ELISA and western immunoblotting the production of antileishmanial IgE antibodies (IgE Ab) in symptomatic and asymptomatic dogs naturally infected by Leishmania chagasi, and also compared this IgE immune response with those of IgG, IgG1 and IgG2 antibodies. Three groups of dogs were evaluated: 12 VL dogs with positive Leishmania biopsies (GI), 44 dogs with a positive leishmanial indirect fluorescent antibody test (IFAT), 30 of them presenting clinical signs of VL and 14 asymptomatic (GII) and 21 healthy dogs living in kennels located in leishmaniasis endemic areas (GIII), which were seronegative in the IFAT. Eighteen dogs from an area free of CVL were used as controls (GIV). Antileishmanial IgE antibodies were detected in 4 of 12 VL dogs from group I (33%) and 14 of 30 symptomatic dogs from group II (47%). While all asymptomatic dogs from group II (100%) were seronegative for antileishmanial IgE Ab, 7 of 21 healthy animals from group III (33%) had these immunoglobulins. A strong correlation was verified between antileishmanial IgG and IgG2 antibody titers in all symptomatic dogs, but only 15 of these 42 animals (36%) produced simultaneously IgE, IgG, IgG1 and IgG2 antibodies to Leishmania. IgE antibodies recognized leishmanial antigens of 12, 36, 61, 81 and 118 KDD, while a more complex pattern of immunoblotting was verified mainly for IgG and IgG2 antibodies from symptomatic animals. IgG1 and IgG2 antibodies shared the recognition of L. chagasi polypeptides of 118, 81, 61, 36, 18, 14 and 12 KDD, being more intense the immune reactions between IgG1 Ab and the leishmanial polypeptides of 61 and 36 KDD, and also between IgG2 antibodies and the antigens of 26, 21, 18, 14 and 12 KDD. Our results suggest that the polyclonal production of antileishmanial antibodies that includes IgE Ab could characterize a Th2 immune response in CVL and can help the laboratory diagnosis of this disease.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-07-17T15:40:05Z No. of bitstreams: 1 (128).pdf: 252129 bytes, checksum: 0e800aa8fe6b85066696676181dcdda0 (MD5)Made available in DSpace on 2012-07-17T15:40:05Z (GMT). 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dc.title.pt_BR.fl_str_mv Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
dc.title.alternative.pt_BR.fl_str_mv Veterinary Immunology and Immunopathology
title Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
spellingShingle Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
Almeida, Maria Angela Ornelas de
Canine visceral leishmaniasis
Leishmania chagasi
IgE antibody
title_short Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
title_full Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
title_fullStr Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
title_full_unstemmed Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
title_sort Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi
author Almeida, Maria Angela Ornelas de
author_facet Almeida, Maria Angela Ornelas de
Jesus, E. E. V.
Atta, Maria Luiza Brito de Sousa
Alves, L. C.
Berne, M. E. A.
Atta, Ajax Mercês
author_role author
author2 Jesus, E. E. V.
Atta, Maria Luiza Brito de Sousa
Alves, L. C.
Berne, M. E. A.
Atta, Ajax Mercês
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Maria Angela Ornelas de
Jesus, E. E. V.
Atta, Maria Luiza Brito de Sousa
Alves, L. C.
Berne, M. E. A.
Atta, Ajax Mercês
Almeida, Maria Angela Ornelas de
Jesus, E. E. V.
Atta, Maria Luiza Brito de Sousa
Alves, L. C.
Berne, M. E. A.
Atta, Ajax Mercês
dc.subject.por.fl_str_mv Canine visceral leishmaniasis
Leishmania chagasi
IgE antibody
topic Canine visceral leishmaniasis
Leishmania chagasi
IgE antibody
description Trabalho completo: acesso restrito, p.151–158
publishDate 2005
dc.date.issued.fl_str_mv 2005-06-15
dc.date.accessioned.fl_str_mv 2012-07-17T15:40:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6444
dc.identifier.issn.none.fl_str_mv 0165-2427
dc.identifier.number.pt_BR.fl_str_mv v. 106, n. 1–2
identifier_str_mv 0165-2427
v. 106, n. 1–2
url http://www.repositorio.ufba.br/ri/handle/ri/6444
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.pt_BR.fl_str_mv ttp://dx.doi.org/10.1016/j.vetimm.2004.08.024
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