Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/7458 https://doi.org/10.2147/JPR.S36870 |
Resumo: | Background: Celecoxib exerted analgesic effects (hypoalgesia) reversed by opioid receptor antagonists in a model of inflammatory pain. To analyze this celecoxib-induced hypoalgesia further, we assessed the effects of several disruptors of cytoskeletal components in our model of inflammation. Methods: Hyperalgesia to mechanical stimuli was induced in rat hind paws by intraplantar injection of carrageenan and measured using the Randall-Selitto method over the next 8 hours. The effects of systemic pretreatment with celecoxib and a range of cytoskeletal disruptors (colchicine, nocodazole, cytochalasin B, latrunculin B, acrylamide, each given by intraplantar injection) on carrageenan-induced hyperalgesia were similarly investigated. Morphine and other selective cyclo-oxygenase 1 (SC-560), cyclo-oxygenase 2 (SC-236), and nonselective cyclo-oxygenase (indomethacin) inhibitors were also tested under similar conditions. Results: None of the cytoskeletal disruptors affected the peak intensity of carrageenan-induced hyperalgesia, and its duration was increased only by nocodazole and colchicine. Pretreatment with celecoxib 30 minutes before carrageenan reversed the hyperalgesia and raised the nociceptive threshold (hypoalgesia). All analgesic effects of celecoxib were blocked by nocodazole, colchicine, cytochalasin B, and latrunculin B. Pretreatment with morphine also induced hypoalgesia in carrageenan-inflamed paws, an effect reversed by colchicine and cytochalasin B. However, the analgesic effects of indomethacin were not reversed by disruption of actin filaments with cytochalasin B or latrunculin B. Conclusion: These data strengthen the correlation between cytoskeletal structures and the processes of pain and analgesia. |
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Lima, Patrícia PaivaRezende, Rafael MachadoLeite, RomuloDuarte, Igor Dimitri GamaBakhle, Yeshwant ShriharshFrancischi, Janetti Nogueira de2017-03-24T16:19:49Z2017-03-24T16:19:49Z2012LIMA, P. P. et al. Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. Journal of Pain Research, p. 535-545, 2012. Disponível em: <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500925/pdf/jpr-5-535.pdf>. Acesso em: 10 jan. 2017.1178-7090http://www.repositorio.ufop.br/handle/123456789/7458https://doi.org/10.2147/JPR.S36870Background: Celecoxib exerted analgesic effects (hypoalgesia) reversed by opioid receptor antagonists in a model of inflammatory pain. To analyze this celecoxib-induced hypoalgesia further, we assessed the effects of several disruptors of cytoskeletal components in our model of inflammation. Methods: Hyperalgesia to mechanical stimuli was induced in rat hind paws by intraplantar injection of carrageenan and measured using the Randall-Selitto method over the next 8 hours. The effects of systemic pretreatment with celecoxib and a range of cytoskeletal disruptors (colchicine, nocodazole, cytochalasin B, latrunculin B, acrylamide, each given by intraplantar injection) on carrageenan-induced hyperalgesia were similarly investigated. Morphine and other selective cyclo-oxygenase 1 (SC-560), cyclo-oxygenase 2 (SC-236), and nonselective cyclo-oxygenase (indomethacin) inhibitors were also tested under similar conditions. Results: None of the cytoskeletal disruptors affected the peak intensity of carrageenan-induced hyperalgesia, and its duration was increased only by nocodazole and colchicine. Pretreatment with celecoxib 30 minutes before carrageenan reversed the hyperalgesia and raised the nociceptive threshold (hypoalgesia). All analgesic effects of celecoxib were blocked by nocodazole, colchicine, cytochalasin B, and latrunculin B. Pretreatment with morphine also induced hypoalgesia in carrageenan-inflamed paws, an effect reversed by colchicine and cytochalasin B. However, the analgesic effects of indomethacin were not reversed by disruption of actin filaments with cytochalasin B or latrunculin B. Conclusion: These data strengthen the correlation between cytoskeletal structures and the processes of pain and analgesia.This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. Fonte: o próprio artigo.info:eu-repo/semantics/openAccessHypoalgesiaCytoskeletonCyclo-oxygenasesCrucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/7458/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_CrucialInvolvementActin.pdfARTIGO_CrucialInvolvementActin.pdfapplication/pdf428716http://www.repositorio.ufop.br/bitstream/123456789/7458/1/ARTIGO_CrucialInvolvementActin.pdf66233222ae914d6f3c8f3e81033060e4MD51123456789/74582019-11-06 11:11:50.883oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-11-06T16:11:50Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. |
title |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. |
spellingShingle |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. Lima, Patrícia Paiva Hypoalgesia Cytoskeleton Cyclo-oxygenases |
title_short |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. |
title_full |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. |
title_fullStr |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. |
title_full_unstemmed |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. |
title_sort |
Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. |
author |
Lima, Patrícia Paiva |
author_facet |
Lima, Patrícia Paiva Rezende, Rafael Machado Leite, Romulo Duarte, Igor Dimitri Gama Bakhle, Yeshwant Shriharsh Francischi, Janetti Nogueira de |
author_role |
author |
author2 |
Rezende, Rafael Machado Leite, Romulo Duarte, Igor Dimitri Gama Bakhle, Yeshwant Shriharsh Francischi, Janetti Nogueira de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Patrícia Paiva Rezende, Rafael Machado Leite, Romulo Duarte, Igor Dimitri Gama Bakhle, Yeshwant Shriharsh Francischi, Janetti Nogueira de |
dc.subject.por.fl_str_mv |
Hypoalgesia Cytoskeleton Cyclo-oxygenases |
topic |
Hypoalgesia Cytoskeleton Cyclo-oxygenases |
description |
Background: Celecoxib exerted analgesic effects (hypoalgesia) reversed by opioid receptor antagonists in a model of inflammatory pain. To analyze this celecoxib-induced hypoalgesia further, we assessed the effects of several disruptors of cytoskeletal components in our model of inflammation. Methods: Hyperalgesia to mechanical stimuli was induced in rat hind paws by intraplantar injection of carrageenan and measured using the Randall-Selitto method over the next 8 hours. The effects of systemic pretreatment with celecoxib and a range of cytoskeletal disruptors (colchicine, nocodazole, cytochalasin B, latrunculin B, acrylamide, each given by intraplantar injection) on carrageenan-induced hyperalgesia were similarly investigated. Morphine and other selective cyclo-oxygenase 1 (SC-560), cyclo-oxygenase 2 (SC-236), and nonselective cyclo-oxygenase (indomethacin) inhibitors were also tested under similar conditions. Results: None of the cytoskeletal disruptors affected the peak intensity of carrageenan-induced hyperalgesia, and its duration was increased only by nocodazole and colchicine. Pretreatment with celecoxib 30 minutes before carrageenan reversed the hyperalgesia and raised the nociceptive threshold (hypoalgesia). All analgesic effects of celecoxib were blocked by nocodazole, colchicine, cytochalasin B, and latrunculin B. Pretreatment with morphine also induced hypoalgesia in carrageenan-inflamed paws, an effect reversed by colchicine and cytochalasin B. However, the analgesic effects of indomethacin were not reversed by disruption of actin filaments with cytochalasin B or latrunculin B. Conclusion: These data strengthen the correlation between cytoskeletal structures and the processes of pain and analgesia. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2017-03-24T16:19:49Z |
dc.date.available.fl_str_mv |
2017-03-24T16:19:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, P. P. et al. Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. Journal of Pain Research, p. 535-545, 2012. Disponível em: <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500925/pdf/jpr-5-535.pdf>. Acesso em: 10 jan. 2017. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/7458 |
dc.identifier.issn.none.fl_str_mv |
1178-7090 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.2147/JPR.S36870 |
identifier_str_mv |
LIMA, P. P. et al. Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain. Journal of Pain Research, p. 535-545, 2012. Disponível em: <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500925/pdf/jpr-5-535.pdf>. Acesso em: 10 jan. 2017. 1178-7090 |
url |
http://www.repositorio.ufop.br/handle/123456789/7458 https://doi.org/10.2147/JPR.S36870 |
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eng |
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