Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/3998 http://dx.doi.org/10.1590/0037-8682-0077-2013 |
Resumo: | The biological diversity of Trypanosoma cruzi strains plays an important role in the clinical and epidemiological features of Chagas disease. Methods: Eight T. cruzi strains isolated from children living in a Chagas disease vector-controlled area of Jequitinhonha Valley, State of Minas Gerais, Brazil, were genetically and biologically characterized. Results: The characterizations demonstrated that all of the strains belonged to T. cruzi II, and showed high infectivity and a variable mean maximum peak of parasitemia. Six strains displayed low parasitemia, and two displayed moderate parasitemia. Later peaks of parasitemia and a predominance of intermediate and large trypomastigotes in all T. cruzi strains were observed. The mean prepatent period was relatively short (4.2±0.25 to 13.7±3.08 days), whereas the patent period ranged from 3.3±1.08 to 34.5±3.52 days. Mortality was observed only in animals infected with strain 806 (62.5%). Histopathological analysis of the heart showed that strains 501 and 806 caused infl ammation, but fi brosis was observed only in animals infected with strain 806. Conclusions: The results indicate the presence of an association between the biological behavior in mice and the genetic characteristics of the parasites. The study also confi rmed general data from Brazil where T. cruzi II lineage is the most prevalent in the domiciliary cycle and generally has low virulence, with some strains capable of inducing infl ammatory processes and fi brosis. |
id |
UFOP_440c6f6ea13dd071c57eb7d22008e304 |
---|---|
oai_identifier_str |
oai:localhost:123456789/3998 |
network_acronym_str |
UFOP |
network_name_str |
Repositório Institucional da UFOP |
repository_id_str |
3233 |
spelling |
Silva, Jaquelline Carla Valamiel de Oliveira eAssis, Girley Francisco Machado deOliveira, Maykon Tavares deValadares, Helder Magno SilvaValle, Ítalo Faria doPaiva, Nívia Carolina Nogueira deMartins, Helen RodriguesLana, Marta de2014-11-20T15:48:44Z2014-11-20T15:48:44Z2013SILVA, J. C. V. de O. e et al. Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. Revista da Sociedade Brasileira de Medicina Tropical v. 46, p. 433-440, 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000400433&lng=en&nrm=iso&tlng=en>. Acesso em: 20 ago. 2014.0037-8682http://www.repositorio.ufop.br/handle/123456789/3998http://dx.doi.org/10.1590/0037-8682-0077-2013The biological diversity of Trypanosoma cruzi strains plays an important role in the clinical and epidemiological features of Chagas disease. Methods: Eight T. cruzi strains isolated from children living in a Chagas disease vector-controlled area of Jequitinhonha Valley, State of Minas Gerais, Brazil, were genetically and biologically characterized. Results: The characterizations demonstrated that all of the strains belonged to T. cruzi II, and showed high infectivity and a variable mean maximum peak of parasitemia. Six strains displayed low parasitemia, and two displayed moderate parasitemia. Later peaks of parasitemia and a predominance of intermediate and large trypomastigotes in all T. cruzi strains were observed. The mean prepatent period was relatively short (4.2±0.25 to 13.7±3.08 days), whereas the patent period ranged from 3.3±1.08 to 34.5±3.52 days. Mortality was observed only in animals infected with strain 806 (62.5%). Histopathological analysis of the heart showed that strains 501 and 806 caused infl ammation, but fi brosis was observed only in animals infected with strain 806. Conclusions: The results indicate the presence of an association between the biological behavior in mice and the genetic characteristics of the parasites. The study also confi rmed general data from Brazil where T. cruzi II lineage is the most prevalent in the domiciliary cycle and generally has low virulence, with some strains capable of inducing infl ammatory processes and fi brosis.Trypanosoma cruzi strainsJequitinhonha ValleyMolecular characterizationBiological characterizationMolecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleA Revista da Sociedade Brasileira de Medicina Tropical permite o depósito das versões pré-print e pós-print de um artigo. Permite remixagem, adaptação e nova criação a partir da obra para fins não comerciais desde que seja atribuído o crédito ao autor (CC BY-NC). Fonte: Diadorim <http://diadorim.ibict.br/handle/1/127>. Acesso em: 10 fev. 2020.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/3998/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_MolecularBiologicalCharacterization.pdfARTIGO_MolecularBiologicalCharacterization.pdfapplication/pdf1662354http://www.repositorio.ufop.br/bitstream/123456789/3998/1/ARTIGO_MolecularBiologicalCharacterization.pdf021c7473b063e200b68022e713e3aaa3MD51123456789/39982020-03-10 09:45:48.703oai:localhost:123456789/3998Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-03-10T13:45:48Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. |
title |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. |
spellingShingle |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. Silva, Jaquelline Carla Valamiel de Oliveira e Trypanosoma cruzi strains Jequitinhonha Valley Molecular characterization Biological characterization |
title_short |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. |
title_full |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. |
title_fullStr |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. |
title_full_unstemmed |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. |
title_sort |
Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. |
author |
Silva, Jaquelline Carla Valamiel de Oliveira e |
author_facet |
Silva, Jaquelline Carla Valamiel de Oliveira e Assis, Girley Francisco Machado de Oliveira, Maykon Tavares de Valadares, Helder Magno Silva Valle, Ítalo Faria do Paiva, Nívia Carolina Nogueira de Martins, Helen Rodrigues Lana, Marta de |
author_role |
author |
author2 |
Assis, Girley Francisco Machado de Oliveira, Maykon Tavares de Valadares, Helder Magno Silva Valle, Ítalo Faria do Paiva, Nívia Carolina Nogueira de Martins, Helen Rodrigues Lana, Marta de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Jaquelline Carla Valamiel de Oliveira e Assis, Girley Francisco Machado de Oliveira, Maykon Tavares de Valadares, Helder Magno Silva Valle, Ítalo Faria do Paiva, Nívia Carolina Nogueira de Martins, Helen Rodrigues Lana, Marta de |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi strains Jequitinhonha Valley Molecular characterization Biological characterization |
topic |
Trypanosoma cruzi strains Jequitinhonha Valley Molecular characterization Biological characterization |
description |
The biological diversity of Trypanosoma cruzi strains plays an important role in the clinical and epidemiological features of Chagas disease. Methods: Eight T. cruzi strains isolated from children living in a Chagas disease vector-controlled area of Jequitinhonha Valley, State of Minas Gerais, Brazil, were genetically and biologically characterized. Results: The characterizations demonstrated that all of the strains belonged to T. cruzi II, and showed high infectivity and a variable mean maximum peak of parasitemia. Six strains displayed low parasitemia, and two displayed moderate parasitemia. Later peaks of parasitemia and a predominance of intermediate and large trypomastigotes in all T. cruzi strains were observed. The mean prepatent period was relatively short (4.2±0.25 to 13.7±3.08 days), whereas the patent period ranged from 3.3±1.08 to 34.5±3.52 days. Mortality was observed only in animals infected with strain 806 (62.5%). Histopathological analysis of the heart showed that strains 501 and 806 caused infl ammation, but fi brosis was observed only in animals infected with strain 806. Conclusions: The results indicate the presence of an association between the biological behavior in mice and the genetic characteristics of the parasites. The study also confi rmed general data from Brazil where T. cruzi II lineage is the most prevalent in the domiciliary cycle and generally has low virulence, with some strains capable of inducing infl ammatory processes and fi brosis. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-11-20T15:48:44Z |
dc.date.available.fl_str_mv |
2014-11-20T15:48:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, J. C. V. de O. e et al. Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. Revista da Sociedade Brasileira de Medicina Tropical v. 46, p. 433-440, 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000400433&lng=en&nrm=iso&tlng=en>. Acesso em: 20 ago. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/3998 |
dc.identifier.issn.none.fl_str_mv |
0037-8682 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0077-2013 |
identifier_str_mv |
SILVA, J. C. V. de O. e et al. Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil. Revista da Sociedade Brasileira de Medicina Tropical v. 46, p. 433-440, 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000400433&lng=en&nrm=iso&tlng=en>. Acesso em: 20 ago. 2014. 0037-8682 |
url |
http://www.repositorio.ufop.br/handle/123456789/3998 http://dx.doi.org/10.1590/0037-8682-0077-2013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
bitstream.url.fl_str_mv |
http://www.repositorio.ufop.br/bitstream/123456789/3998/2/license.txt http://www.repositorio.ufop.br/bitstream/123456789/3998/1/ARTIGO_MolecularBiologicalCharacterization.pdf |
bitstream.checksum.fl_str_mv |
8a4605be74aa9ea9d79846c1fba20a33 021c7473b063e200b68022e713e3aaa3 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
_version_ |
1801685761509031936 |