Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum.
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/4323 https://doi.org/10.1016/j.ejphar.2008.06.001 |
Resumo: | Protein kinase C (PKC) is involved in the regulation of vascular smooth muscle contraction. However, the role of PKC in erectile function is poorly understood. This study investigated whether PKC mediates agonistinduced contractions in mouse penile tissue (corpora cavernosa). We also compared the effects of PKC activators and inhibitors on contractile responses in mouse corpus cavernosum with those in mouse aorta. Aortic rings and corpus cavernosal strips from C57BL/6J mice were mounted in the organ bath for isometric tension recording. Our data showed that a PKCα/β selective inhibitor, Gö6976 (10 μM), inhibited phenylephrine and 9,11-dideoxy-11α,9α-epoxymethanoprostaglandin F2α (U46619, a thromboxane mimetic)- induced contractions in mouse aorta, reducing the maximum contraction by 94% and 17%, respectively. A non-selective PKC inhibitor, chelerythrine (30 μM), also significantly reduced phenylephrineand U46619-induced maximum contractions in mouse aorta. However, Gö6976 and chelerythrine had no significant effects on phenylephrine- and U46619-induced contractions in corpus cavernosum. Furthermore, a PKC activator, phorbol-12,13-dibutyrate (0.1 μM), significantly increased contractions in aorta (208±14% of KCl-induced maximum contraction) but failed to cause contractions in corpus cavernosum at 1 and 10 μM. Western blot analysis data suggested that protein expression of PKC was similar in aorta and corpus cavernosum. Taken together, our data indicate that PKC does not have a significant role in agonist-induced contractions in mouse corpus cavernosum, whereas it mediates the contractile response to agonists in the aorta. |
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Jin, LimingTeixeira, Cleber E.Webb, Robert ClintonLeite, Romulo2015-01-21T18:36:53Z2015-01-21T18:36:53Z2008JIN, L. et al. Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. European Journal of Pharmacology, v. 590, p. 363-368, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0014299908006122>. Acesso em: 20 ago. 2014.0014-2999http://www.repositorio.ufop.br/handle/123456789/4323https://doi.org/10.1016/j.ejphar.2008.06.001Protein kinase C (PKC) is involved in the regulation of vascular smooth muscle contraction. However, the role of PKC in erectile function is poorly understood. This study investigated whether PKC mediates agonistinduced contractions in mouse penile tissue (corpora cavernosa). We also compared the effects of PKC activators and inhibitors on contractile responses in mouse corpus cavernosum with those in mouse aorta. Aortic rings and corpus cavernosal strips from C57BL/6J mice were mounted in the organ bath for isometric tension recording. Our data showed that a PKCα/β selective inhibitor, Gö6976 (10 μM), inhibited phenylephrine and 9,11-dideoxy-11α,9α-epoxymethanoprostaglandin F2α (U46619, a thromboxane mimetic)- induced contractions in mouse aorta, reducing the maximum contraction by 94% and 17%, respectively. A non-selective PKC inhibitor, chelerythrine (30 μM), also significantly reduced phenylephrineand U46619-induced maximum contractions in mouse aorta. However, Gö6976 and chelerythrine had no significant effects on phenylephrine- and U46619-induced contractions in corpus cavernosum. Furthermore, a PKC activator, phorbol-12,13-dibutyrate (0.1 μM), significantly increased contractions in aorta (208±14% of KCl-induced maximum contraction) but failed to cause contractions in corpus cavernosum at 1 and 10 μM. Western blot analysis data suggested that protein expression of PKC was similar in aorta and corpus cavernosum. Taken together, our data indicate that PKC does not have a significant role in agonist-induced contractions in mouse corpus cavernosum, whereas it mediates the contractile response to agonists in the aorta.ProteinErectileErectileComparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico European Journal of Pharmacology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3456621455220.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/4323/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_ComparisomInvolvementProtein.pdfARTIGO_ComparisomInvolvementProtein.pdfapplication/pdf639670http://www.repositorio.ufop.br/bitstream/123456789/4323/1/ARTIGO_ComparisomInvolvementProtein.pdfdadc7951d804b8af34282c6d1cd77a1aMD51123456789/43232019-06-11 13:25:29.559oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-11T17:25:29Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. |
title |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. |
spellingShingle |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. Jin, Liming Protein Erectile Erectile |
title_short |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. |
title_full |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. |
title_fullStr |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. |
title_full_unstemmed |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. |
title_sort |
Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. |
author |
Jin, Liming |
author_facet |
Jin, Liming Teixeira, Cleber E. Webb, Robert Clinton Leite, Romulo |
author_role |
author |
author2 |
Teixeira, Cleber E. Webb, Robert Clinton Leite, Romulo |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Jin, Liming Teixeira, Cleber E. Webb, Robert Clinton Leite, Romulo |
dc.subject.por.fl_str_mv |
Protein Erectile Erectile |
topic |
Protein Erectile Erectile |
description |
Protein kinase C (PKC) is involved in the regulation of vascular smooth muscle contraction. However, the role of PKC in erectile function is poorly understood. This study investigated whether PKC mediates agonistinduced contractions in mouse penile tissue (corpora cavernosa). We also compared the effects of PKC activators and inhibitors on contractile responses in mouse corpus cavernosum with those in mouse aorta. Aortic rings and corpus cavernosal strips from C57BL/6J mice were mounted in the organ bath for isometric tension recording. Our data showed that a PKCα/β selective inhibitor, Gö6976 (10 μM), inhibited phenylephrine and 9,11-dideoxy-11α,9α-epoxymethanoprostaglandin F2α (U46619, a thromboxane mimetic)- induced contractions in mouse aorta, reducing the maximum contraction by 94% and 17%, respectively. A non-selective PKC inhibitor, chelerythrine (30 μM), also significantly reduced phenylephrineand U46619-induced maximum contractions in mouse aorta. However, Gö6976 and chelerythrine had no significant effects on phenylephrine- and U46619-induced contractions in corpus cavernosum. Furthermore, a PKC activator, phorbol-12,13-dibutyrate (0.1 μM), significantly increased contractions in aorta (208±14% of KCl-induced maximum contraction) but failed to cause contractions in corpus cavernosum at 1 and 10 μM. Western blot analysis data suggested that protein expression of PKC was similar in aorta and corpus cavernosum. Taken together, our data indicate that PKC does not have a significant role in agonist-induced contractions in mouse corpus cavernosum, whereas it mediates the contractile response to agonists in the aorta. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2015-01-21T18:36:53Z |
dc.date.available.fl_str_mv |
2015-01-21T18:36:53Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
JIN, L. et al. Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. European Journal of Pharmacology, v. 590, p. 363-368, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0014299908006122>. Acesso em: 20 ago. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/4323 |
dc.identifier.issn.none.fl_str_mv |
0014-2999 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.ejphar.2008.06.001 |
identifier_str_mv |
JIN, L. et al. Comparison of the involvement of protein kinase C in agonist-induced contractions in mouse aorta and corpus cavernosum. European Journal of Pharmacology, v. 590, p. 363-368, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0014299908006122>. Acesso em: 20 ago. 2014. 0014-2999 |
url |
http://www.repositorio.ufop.br/handle/123456789/4323 https://doi.org/10.1016/j.ejphar.2008.06.001 |
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eng |
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