Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.

Detalhes bibliográficos
Autor(a) principal: Kalapothakis, Evanguedes
Data de Publicação: 2001
Outros Autores: Jardim, S., Magalhães, Ana Cristina Ribeiro, Mendes, Thais Melo, Marco, Luiz de, Afonso, Luís Carlos Crocco, Olórtegui, Carlos Delfin Chávez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4615
https://doi.org/10.1016/S0041-0101(00)00194-X
Resumo: The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing showed 93% similarity with the Tityus bahiensis TbTx IV-5 neurotoxin. The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects.
id UFOP_53f9c971cb0e672da8f4754b8675b501
oai_identifier_str oai:repositorio.ufop.br:123456789/4615
network_acronym_str UFOP
network_name_str Repositório Institucional da UFOP
repository_id_str 3233
spelling Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.ScreeningExpression libraryGenomeProteomeThe present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing showed 93% similarity with the Tityus bahiensis TbTx IV-5 neurotoxin. The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects.2015-03-12T18:54:12Z2015-03-12T18:54:12Z2001info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfKalapothakis, E. et al. Screening of expression libraries using ELISA: identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom. Toxicon, v. 39, p. 679-685, 2001. Disponível em: <http://www.sciencedirect.com/science/article/pii/S004101010000194X>. Acesso em: 08 nov. 2014.0041-0101http://www.repositorio.ufop.br/handle/123456789/4615https://doi.org/10.1016/S0041-0101(00)00194-XO periódico Toxicon concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3530901407398.info:eu-repo/semantics/openAccessKalapothakis, EvanguedesJardim, S.Magalhães, Ana Cristina RibeiroMendes, Thais MeloMarco, Luiz deAfonso, Luís Carlos CroccoOlórtegui, Carlos Delfin Chávezengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2024-11-11T01:48:06Zoai:repositorio.ufop.br:123456789/4615Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332024-11-11T01:48:06Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
title Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
spellingShingle Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
Kalapothakis, Evanguedes
Screening
Expression library
Genome
Proteome
title_short Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
title_full Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
title_fullStr Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
title_full_unstemmed Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
title_sort Screening of expression libraries using ELISA : identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
author Kalapothakis, Evanguedes
author_facet Kalapothakis, Evanguedes
Jardim, S.
Magalhães, Ana Cristina Ribeiro
Mendes, Thais Melo
Marco, Luiz de
Afonso, Luís Carlos Crocco
Olórtegui, Carlos Delfin Chávez
author_role author
author2 Jardim, S.
Magalhães, Ana Cristina Ribeiro
Mendes, Thais Melo
Marco, Luiz de
Afonso, Luís Carlos Crocco
Olórtegui, Carlos Delfin Chávez
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kalapothakis, Evanguedes
Jardim, S.
Magalhães, Ana Cristina Ribeiro
Mendes, Thais Melo
Marco, Luiz de
Afonso, Luís Carlos Crocco
Olórtegui, Carlos Delfin Chávez
dc.subject.por.fl_str_mv Screening
Expression library
Genome
Proteome
topic Screening
Expression library
Genome
Proteome
description The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing showed 93% similarity with the Tityus bahiensis TbTx IV-5 neurotoxin. The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects.
publishDate 2001
dc.date.none.fl_str_mv 2001
2015-03-12T18:54:12Z
2015-03-12T18:54:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Kalapothakis, E. et al. Screening of expression libraries using ELISA: identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom. Toxicon, v. 39, p. 679-685, 2001. Disponível em: <http://www.sciencedirect.com/science/article/pii/S004101010000194X>. Acesso em: 08 nov. 2014.
0041-0101
http://www.repositorio.ufop.br/handle/123456789/4615
https://doi.org/10.1016/S0041-0101(00)00194-X
identifier_str_mv Kalapothakis, E. et al. Screening of expression libraries using ELISA: identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom. Toxicon, v. 39, p. 679-685, 2001. Disponível em: <http://www.sciencedirect.com/science/article/pii/S004101010000194X>. Acesso em: 08 nov. 2014.
0041-0101
url http://www.repositorio.ufop.br/handle/123456789/4615
https://doi.org/10.1016/S0041-0101(00)00194-X
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
_version_ 1823329453725450240

Registros relacionados