Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil).
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/5516 https://doi.org/10.1016/j.mrfmmm.2014.02.006 |
Resumo: | Allyl isothiocyanate (AITC) is present in plants of the cruciferous family and is abundant in mustard seed.Due to its high bioavailability in urine after ingestion, AITC has been considered a promising antineoplasticagent against bladder cancer. Because TP53 mutations are the most common alterations in bladder cancercells and are frequently detected in in situ carcinomas, in this study, we investigated whether the AITCeffects in bladder cancer cells are dependent on the TP53 status. Two bladder transitional carcinoma celllines were used: RT4, with wild-type TP53; and T24, mutated TP53 gene. AITC was tested at concentrationsof 0.005, 0.0625, 0.0725, 0.0825, 0.0925, 0.125 and 0.25 _M in cytotoxicity, cell and clonogenic survivalassays, comet and micronucleus assays and for its effects on cell cycle and apoptosis by flow cytometry andon TP53 gene expression. The data showed increased primary DNA damage in both cell lines; however,lower concentrations of AITC were able to induce genotoxicity in the mutant cells for the TP53 gene.Furthermore, the results demonstrated increased apoptosis and necrosis rates in the wild-type cells, butnot in mutated TP53 cells, and cell cycle arrest in the G2 phase for mutated cells after AITC treatment.No significant differences were detected in TP53 gene expression in the two cell lines. In conclusion,AITC caused cell cycle arrest, increased apoptosis rates and varying genotoxicity dependent on the TP53status. However, we cannot rule out the possibility that those differences could reflect other intrinsicgenetic alterations in the examined cell lines, which may also carry mutations in genes other than TP53.Therefore, further studies using other molecular targets need to be performed to better understand themechanisms by which AITC may exert its antineoplastic properties against tumor cells. |
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Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil).Allyl isothiocyanateApoptosisBladder cancerCell cycleGenotoxicityAllyl isothiocyanate (AITC) is present in plants of the cruciferous family and is abundant in mustard seed.Due to its high bioavailability in urine after ingestion, AITC has been considered a promising antineoplasticagent against bladder cancer. Because TP53 mutations are the most common alterations in bladder cancercells and are frequently detected in in situ carcinomas, in this study, we investigated whether the AITCeffects in bladder cancer cells are dependent on the TP53 status. Two bladder transitional carcinoma celllines were used: RT4, with wild-type TP53; and T24, mutated TP53 gene. AITC was tested at concentrationsof 0.005, 0.0625, 0.0725, 0.0825, 0.0925, 0.125 and 0.25 _M in cytotoxicity, cell and clonogenic survivalassays, comet and micronucleus assays and for its effects on cell cycle and apoptosis by flow cytometry andon TP53 gene expression. The data showed increased primary DNA damage in both cell lines; however,lower concentrations of AITC were able to induce genotoxicity in the mutant cells for the TP53 gene.Furthermore, the results demonstrated increased apoptosis and necrosis rates in the wild-type cells, butnot in mutated TP53 cells, and cell cycle arrest in the G2 phase for mutated cells after AITC treatment.No significant differences were detected in TP53 gene expression in the two cell lines. In conclusion,AITC caused cell cycle arrest, increased apoptosis rates and varying genotoxicity dependent on the TP53status. However, we cannot rule out the possibility that those differences could reflect other intrinsicgenetic alterations in the examined cell lines, which may also carry mutations in genes other than TP53.Therefore, further studies using other molecular targets need to be performed to better understand themechanisms by which AITC may exert its antineoplastic properties against tumor cells.2015-05-26T18:29:35Z2015-05-26T18:29:35Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSÁVIO, A. L. V. et al. Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). Mutation Research, v. 762, p. 40-46, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0027510714000451>. Acesso em: 22 mai. 2015.0027-5107http://www.repositorio.ufop.br/handle/123456789/5516https://doi.org/10.1016/j.mrfmmm.2014.02.006O periódico Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3635910980871.info:eu-repo/semantics/openAccessSávio, André Luiz VenturaSilva, Glenda Nicioli daCamargo, Elaine Aparecida deSalvadori, Daisy Maria Fáveroengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-07-26T15:14:58Zoai:repositorio.ufop.br:123456789/5516Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-07-26T15:14:58Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). |
title |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). |
spellingShingle |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). Sávio, André Luiz Ventura Allyl isothiocyanate Apoptosis Bladder cancer Cell cycle Genotoxicity |
title_short |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). |
title_full |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). |
title_fullStr |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). |
title_full_unstemmed |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). |
title_sort |
Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). |
author |
Sávio, André Luiz Ventura |
author_facet |
Sávio, André Luiz Ventura Silva, Glenda Nicioli da Camargo, Elaine Aparecida de Salvadori, Daisy Maria Fávero |
author_role |
author |
author2 |
Silva, Glenda Nicioli da Camargo, Elaine Aparecida de Salvadori, Daisy Maria Fávero |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Sávio, André Luiz Ventura Silva, Glenda Nicioli da Camargo, Elaine Aparecida de Salvadori, Daisy Maria Fávero |
dc.subject.por.fl_str_mv |
Allyl isothiocyanate Apoptosis Bladder cancer Cell cycle Genotoxicity |
topic |
Allyl isothiocyanate Apoptosis Bladder cancer Cell cycle Genotoxicity |
description |
Allyl isothiocyanate (AITC) is present in plants of the cruciferous family and is abundant in mustard seed.Due to its high bioavailability in urine after ingestion, AITC has been considered a promising antineoplasticagent against bladder cancer. Because TP53 mutations are the most common alterations in bladder cancercells and are frequently detected in in situ carcinomas, in this study, we investigated whether the AITCeffects in bladder cancer cells are dependent on the TP53 status. Two bladder transitional carcinoma celllines were used: RT4, with wild-type TP53; and T24, mutated TP53 gene. AITC was tested at concentrationsof 0.005, 0.0625, 0.0725, 0.0825, 0.0925, 0.125 and 0.25 _M in cytotoxicity, cell and clonogenic survivalassays, comet and micronucleus assays and for its effects on cell cycle and apoptosis by flow cytometry andon TP53 gene expression. The data showed increased primary DNA damage in both cell lines; however,lower concentrations of AITC were able to induce genotoxicity in the mutant cells for the TP53 gene.Furthermore, the results demonstrated increased apoptosis and necrosis rates in the wild-type cells, butnot in mutated TP53 cells, and cell cycle arrest in the G2 phase for mutated cells after AITC treatment.No significant differences were detected in TP53 gene expression in the two cell lines. In conclusion,AITC caused cell cycle arrest, increased apoptosis rates and varying genotoxicity dependent on the TP53status. However, we cannot rule out the possibility that those differences could reflect other intrinsicgenetic alterations in the examined cell lines, which may also carry mutations in genes other than TP53.Therefore, further studies using other molecular targets need to be performed to better understand themechanisms by which AITC may exert its antineoplastic properties against tumor cells. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-05-26T18:29:35Z 2015-05-26T18:29:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SÁVIO, A. L. V. et al. Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). Mutation Research, v. 762, p. 40-46, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0027510714000451>. Acesso em: 22 mai. 2015. 0027-5107 http://www.repositorio.ufop.br/handle/123456789/5516 https://doi.org/10.1016/j.mrfmmm.2014.02.006 |
identifier_str_mv |
SÁVIO, A. L. V. et al. Cell cycle kinetics, apoptosis rates, DNA damage and TP53 geneexpression in bladder cancer cells treated with allyl isothiocyanate(mustard essential oil). Mutation Research, v. 762, p. 40-46, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0027510714000451>. Acesso em: 22 mai. 2015. 0027-5107 |
url |
http://www.repositorio.ufop.br/handle/123456789/5516 https://doi.org/10.1016/j.mrfmmm.2014.02.006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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1813002819079241728 |