Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/4312 https://doi.org/10.1371/journal.pone.0091009 |
Resumo: | The techniques used for diagnosis of canine visceral leishmaniasis (CVL) in Brazil ELISA and IFAT have been extensively questioned because of the accuracy of these tests. A recent change in the diagnosis protocol excluded IFAT and included the Dual-Path Platform (DPP). We evaluated the prevalence and incidence rates of Leishmania spp. before and after the change in the protocol. In addition, based on our results, we propose a new alternative that is less expensive for the screening and confirmation of CVL. Plasma samples were obtained from a serobank from dogs evaluated in a cross-sectional study (1,226 dogs) and in a cohort study of susceptible animals (n = 447), followed for 26 months. Serology testing wasperformed using ELISA, IFAT, and DPP. The incidence and prevalence of CVL were determined by using the protocol of the Visceral Leishmaniasis Control and Surveillance Program until 2012 (ELISA and IFAT using filter paper) and the protocol used after 2012 (DPP and ELISA using plasma). The prevalence was 6.2% and the incidence was 2.8 per 1,000 dog-months for the protocol used until 2012. For the new diagnosis protocol for CVL resulted in an incidence of 5.4 per 1,000 dog-months and a prevalence of 8.1%. Our results showed that the prevalence and incidence of infection were far greater than suggested by the previously used protocol and that the magnitude of infection in endemic areas has been underestimated. As tests are performed sequentially and euthanasia of dogs is carried out when the serological results are positive in both tests, the sequence does not affect the number of animals to be eliminated by the Control Program. Then we suggest to municipalities with a large demand of exams to use ELISA for screening and DPP for confirmation, since this allows easier performance and reduced cost. |
id |
UFOP_8ad1046ebefe0c3efe717c9a90edc1bf |
---|---|
oai_identifier_str |
oai:repositorio.ufop.br:123456789/4312 |
network_acronym_str |
UFOP |
network_name_str |
Repositório Institucional da UFOP |
repository_id_str |
3233 |
spelling |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis.Teste - ELISACanine visceral leishmaniasisThe techniques used for diagnosis of canine visceral leishmaniasis (CVL) in Brazil ELISA and IFAT have been extensively questioned because of the accuracy of these tests. A recent change in the diagnosis protocol excluded IFAT and included the Dual-Path Platform (DPP). We evaluated the prevalence and incidence rates of Leishmania spp. before and after the change in the protocol. In addition, based on our results, we propose a new alternative that is less expensive for the screening and confirmation of CVL. Plasma samples were obtained from a serobank from dogs evaluated in a cross-sectional study (1,226 dogs) and in a cohort study of susceptible animals (n = 447), followed for 26 months. Serology testing wasperformed using ELISA, IFAT, and DPP. The incidence and prevalence of CVL were determined by using the protocol of the Visceral Leishmaniasis Control and Surveillance Program until 2012 (ELISA and IFAT using filter paper) and the protocol used after 2012 (DPP and ELISA using plasma). The prevalence was 6.2% and the incidence was 2.8 per 1,000 dog-months for the protocol used until 2012. For the new diagnosis protocol for CVL resulted in an incidence of 5.4 per 1,000 dog-months and a prevalence of 8.1%. Our results showed that the prevalence and incidence of infection were far greater than suggested by the previously used protocol and that the magnitude of infection in endemic areas has been underestimated. As tests are performed sequentially and euthanasia of dogs is carried out when the serological results are positive in both tests, the sequence does not affect the number of animals to be eliminated by the Control Program. Then we suggest to municipalities with a large demand of exams to use ELISA for screening and DPP for confirmation, since this allows easier performance and reduced cost.2015-01-21T18:19:55Z2015-01-21T18:19:55Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVITAL, W. C. et al. Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. Plos One, v. 9, n. 3, p. e91009, 2014. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091009>. Acesso em: 12 ago. 2014.1932-6203http://www.repositorio.ufop.br/handle/123456789/4312https://doi.org/10.1371/journal.pone.0091009Os trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <https://www.plos.org/open-access>. Acesso em: 03 jan. 2017.info:eu-repo/semantics/openAccessVital, Wendel CouraKer, Henrique GamaRoatt, Bruno MendesSoares, Rodrigo Dian de Oliveira AguiarLeal, Gleisiane Gomes de AlmeidaMoreira, Nádia das DoresOliveira, Laser Antônio Machado deMachado, Evandro Marques de MenezesMorais, Maria Helena FrancoOliveira, Rodrigo Corrêa deCarneiro, MariângelaReis, Alexandre Barbosaengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-11T16:56:45Zoai:repositorio.ufop.br:123456789/4312Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-11T16:56:45Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. |
title |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. |
spellingShingle |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. Vital, Wendel Coura Teste - ELISA Canine visceral leishmaniasis |
title_short |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. |
title_full |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. |
title_fullStr |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. |
title_full_unstemmed |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. |
title_sort |
Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. |
author |
Vital, Wendel Coura |
author_facet |
Vital, Wendel Coura Ker, Henrique Gama Roatt, Bruno Mendes Soares, Rodrigo Dian de Oliveira Aguiar Leal, Gleisiane Gomes de Almeida Moreira, Nádia das Dores Oliveira, Laser Antônio Machado de Machado, Evandro Marques de Menezes Morais, Maria Helena Franco Oliveira, Rodrigo Corrêa de Carneiro, Mariângela Reis, Alexandre Barbosa |
author_role |
author |
author2 |
Ker, Henrique Gama Roatt, Bruno Mendes Soares, Rodrigo Dian de Oliveira Aguiar Leal, Gleisiane Gomes de Almeida Moreira, Nádia das Dores Oliveira, Laser Antônio Machado de Machado, Evandro Marques de Menezes Morais, Maria Helena Franco Oliveira, Rodrigo Corrêa de Carneiro, Mariângela Reis, Alexandre Barbosa |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vital, Wendel Coura Ker, Henrique Gama Roatt, Bruno Mendes Soares, Rodrigo Dian de Oliveira Aguiar Leal, Gleisiane Gomes de Almeida Moreira, Nádia das Dores Oliveira, Laser Antônio Machado de Machado, Evandro Marques de Menezes Morais, Maria Helena Franco Oliveira, Rodrigo Corrêa de Carneiro, Mariângela Reis, Alexandre Barbosa |
dc.subject.por.fl_str_mv |
Teste - ELISA Canine visceral leishmaniasis |
topic |
Teste - ELISA Canine visceral leishmaniasis |
description |
The techniques used for diagnosis of canine visceral leishmaniasis (CVL) in Brazil ELISA and IFAT have been extensively questioned because of the accuracy of these tests. A recent change in the diagnosis protocol excluded IFAT and included the Dual-Path Platform (DPP). We evaluated the prevalence and incidence rates of Leishmania spp. before and after the change in the protocol. In addition, based on our results, we propose a new alternative that is less expensive for the screening and confirmation of CVL. Plasma samples were obtained from a serobank from dogs evaluated in a cross-sectional study (1,226 dogs) and in a cohort study of susceptible animals (n = 447), followed for 26 months. Serology testing wasperformed using ELISA, IFAT, and DPP. The incidence and prevalence of CVL were determined by using the protocol of the Visceral Leishmaniasis Control and Surveillance Program until 2012 (ELISA and IFAT using filter paper) and the protocol used after 2012 (DPP and ELISA using plasma). The prevalence was 6.2% and the incidence was 2.8 per 1,000 dog-months for the protocol used until 2012. For the new diagnosis protocol for CVL resulted in an incidence of 5.4 per 1,000 dog-months and a prevalence of 8.1%. Our results showed that the prevalence and incidence of infection were far greater than suggested by the previously used protocol and that the magnitude of infection in endemic areas has been underestimated. As tests are performed sequentially and euthanasia of dogs is carried out when the serological results are positive in both tests, the sequence does not affect the number of animals to be eliminated by the Control Program. Then we suggest to municipalities with a large demand of exams to use ELISA for screening and DPP for confirmation, since this allows easier performance and reduced cost. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-01-21T18:19:55Z 2015-01-21T18:19:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VITAL, W. C. et al. Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. Plos One, v. 9, n. 3, p. e91009, 2014. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091009>. Acesso em: 12 ago. 2014. 1932-6203 http://www.repositorio.ufop.br/handle/123456789/4312 https://doi.org/10.1371/journal.pone.0091009 |
identifier_str_mv |
VITAL, W. C. et al. Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. Plos One, v. 9, n. 3, p. e91009, 2014. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091009>. Acesso em: 12 ago. 2014. 1932-6203 |
url |
http://www.repositorio.ufop.br/handle/123456789/4312 https://doi.org/10.1371/journal.pone.0091009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
_version_ |
1813002800303439872 |