Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/5213 https://doi.org/10.1016/j.foodres.2012.03.003 |
Resumo: | This paper describes the investigation of the cytotoxic and antioxidant activities and in vivo mutagenic/ antimutagenic potential of different concentrations of the hexane extract (EHP) and isolated molecule 7-epi-clusianone (MI) of Rheedia brasiliensis. The in vitro antioxidant activity of MI was investigated by monitoring the reduction of radical scavenging and metal chelating activity of DPPH (1,1-diphenyl-2-picrylhydrazil). Cytotoxic activity was assessed by measuring the mortality of brine shrimp in the presence and absence of the compounds. The mutagenic, antimutagenic and cytotoxic effects of these compounds were evaluated by a micronucleus test. During the antioxidant activity assessment, the 7-epi-clusianone was significantly higher than that of EHP at all concentrations in three assays. From the results obtained with the assessment of cytotoxic activity, all samples had a mortality rate (LC50b100 mg/mL) lower than the positive control (thymol). The results of the micronucleus test revealed that MI at 5, 10 and 15 mg/kg b.w. is antimutagenic. In conclusion, these results suggest that in the future, the EHP and MI could be used as prophylactic agents in cancer prevention. |
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Silva, Luciano Bruno CarvalhoOliveira, Maysa do ValeGontijo, Vanessa SilvaOliveira, Williana Fernandes deDerogis, Priscilla Bento Matos CruzStringheta, Paulo CésarNagem, Tanus JorgeBrigagão, Maísa R. P. L.Santos, Marcelo Henrique dos2015-04-30T13:58:06Z2015-04-30T13:58:06Z2012SILVA, L. B. C. et al. Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. Food Research International, v. 48, p. 180-186, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0963996912000981>. Acesso em: 19 fev. 2015.0963-9969http://www.repositorio.ufop.br/handle/123456789/5213https://doi.org/10.1016/j.foodres.2012.03.003This paper describes the investigation of the cytotoxic and antioxidant activities and in vivo mutagenic/ antimutagenic potential of different concentrations of the hexane extract (EHP) and isolated molecule 7-epi-clusianone (MI) of Rheedia brasiliensis. The in vitro antioxidant activity of MI was investigated by monitoring the reduction of radical scavenging and metal chelating activity of DPPH (1,1-diphenyl-2-picrylhydrazil). Cytotoxic activity was assessed by measuring the mortality of brine shrimp in the presence and absence of the compounds. The mutagenic, antimutagenic and cytotoxic effects of these compounds were evaluated by a micronucleus test. During the antioxidant activity assessment, the 7-epi-clusianone was significantly higher than that of EHP at all concentrations in three assays. From the results obtained with the assessment of cytotoxic activity, all samples had a mortality rate (LC50b100 mg/mL) lower than the positive control (thymol). The results of the micronucleus test revealed that MI at 5, 10 and 15 mg/kg b.w. is antimutagenic. In conclusion, these results suggest that in the future, the EHP and MI could be used as prophylactic agents in cancer prevention.Medicinal plantsAntioxidantMicronucleus testMutationsAntioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Food Research International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3582471067314.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-82636http://www.repositorio.ufop.br/bitstream/123456789/5213/2/license.txtc2ffdd99e58acf69202dff00d361f23aMD52ORIGINALARTIGO_AntioxidantCytotoxicAntimutagenic.pdfARTIGO_AntioxidantCytotoxicAntimutagenic.pdfapplication/pdf276531http://www.repositorio.ufop.br/bitstream/123456789/5213/1/ARTIGO_AntioxidantCytotoxicAntimutagenic.pdfe152025c069c1d6f36aa761ceee5e16dMD51123456789/52132019-07-16 12:57:57.779oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-07-16T16:57:57Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. |
title |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. |
spellingShingle |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. Silva, Luciano Bruno Carvalho Medicinal plants Antioxidant Micronucleus test Mutations |
title_short |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. |
title_full |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. |
title_fullStr |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. |
title_full_unstemmed |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. |
title_sort |
Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. |
author |
Silva, Luciano Bruno Carvalho |
author_facet |
Silva, Luciano Bruno Carvalho Oliveira, Maysa do Vale Gontijo, Vanessa Silva Oliveira, Williana Fernandes de Derogis, Priscilla Bento Matos Cruz Stringheta, Paulo César Nagem, Tanus Jorge Brigagão, Maísa R. P. L. Santos, Marcelo Henrique dos |
author_role |
author |
author2 |
Oliveira, Maysa do Vale Gontijo, Vanessa Silva Oliveira, Williana Fernandes de Derogis, Priscilla Bento Matos Cruz Stringheta, Paulo César Nagem, Tanus Jorge Brigagão, Maísa R. P. L. Santos, Marcelo Henrique dos |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Luciano Bruno Carvalho Oliveira, Maysa do Vale Gontijo, Vanessa Silva Oliveira, Williana Fernandes de Derogis, Priscilla Bento Matos Cruz Stringheta, Paulo César Nagem, Tanus Jorge Brigagão, Maísa R. P. L. Santos, Marcelo Henrique dos |
dc.subject.por.fl_str_mv |
Medicinal plants Antioxidant Micronucleus test Mutations |
topic |
Medicinal plants Antioxidant Micronucleus test Mutations |
description |
This paper describes the investigation of the cytotoxic and antioxidant activities and in vivo mutagenic/ antimutagenic potential of different concentrations of the hexane extract (EHP) and isolated molecule 7-epi-clusianone (MI) of Rheedia brasiliensis. The in vitro antioxidant activity of MI was investigated by monitoring the reduction of radical scavenging and metal chelating activity of DPPH (1,1-diphenyl-2-picrylhydrazil). Cytotoxic activity was assessed by measuring the mortality of brine shrimp in the presence and absence of the compounds. The mutagenic, antimutagenic and cytotoxic effects of these compounds were evaluated by a micronucleus test. During the antioxidant activity assessment, the 7-epi-clusianone was significantly higher than that of EHP at all concentrations in three assays. From the results obtained with the assessment of cytotoxic activity, all samples had a mortality rate (LC50b100 mg/mL) lower than the positive control (thymol). The results of the micronucleus test revealed that MI at 5, 10 and 15 mg/kg b.w. is antimutagenic. In conclusion, these results suggest that in the future, the EHP and MI could be used as prophylactic agents in cancer prevention. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2015-04-30T13:58:06Z |
dc.date.available.fl_str_mv |
2015-04-30T13:58:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, L. B. C. et al. Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. Food Research International, v. 48, p. 180-186, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0963996912000981>. Acesso em: 19 fev. 2015. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/5213 |
dc.identifier.issn.none.fl_str_mv |
0963-9969 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.foodres.2012.03.003 |
identifier_str_mv |
SILVA, L. B. C. et al. Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis. Food Research International, v. 48, p. 180-186, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0963996912000981>. Acesso em: 19 fev. 2015. 0963-9969 |
url |
http://www.repositorio.ufop.br/handle/123456789/5213 https://doi.org/10.1016/j.foodres.2012.03.003 |
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eng |
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