Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/11071 |
Resumo: | This study evaluates whether intrathecal MVIIA injection after spinal cord injury (SCI) elicits neuroprotective effects. The test rats were randomly distributed into six groups— sham, placebo, MVIIA 2.5 μM, MVIIA 5 μM, MVIIA 10 μM, and MVIIA 20 μM—and were administered the treatment four hours after SCI. After the optimal MVIIA dose (MVIIA 10 μM) was defined, the best time for application, one or four hours, was analyzed. Locomotor hind limb function and side effects were assessed. Forty-eight hours after the injury and immediately after euthanasia, spinal cord segments were removed from the test rats. Cell viability, reactive oxygen species, lipid peroxidation, and glutamate release were investigated. To examine the MVIIA mechanism of action, the gene expressions of pro-apoptotic (Bax, nNOS, and caspase-3, -8, -9, -12) and anti-apoptotic (Bcl-xl) factors in the spinal cord tissue samples were determined by real-time PCR, and the activities of antioxidant enzymes were also investigated. Application of intrathecal MVIIA 10 μM four hours after SCI prompted a neuroprotective effect: neuronal death decreased (22.46%), oxidative stress diminished, pro-apoptotic factors (Bax, nNOS, and caspase-3, -8) were expressed to a lesser extent, and mitochondrial viability as well as anti-apoptotic factor (Bcl-xl) expression increased. These results suggested that MVIIA provided neuroprotection through antioxidant effects. Indeed, superoxide dismutase (188.41%), and glutathione peroxidase (199.96%), reductase (193.86%), and transferase (175.93%) expressions increased. Therefore, intrathecal MVIIA (MVIIA 10 μM, 4 h) application has neuroprotective potential, and the possible mechanisms are related to antioxidant agent modulation and to intrinsic and extrinsic apoptotic pathways. |
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Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats.This study evaluates whether intrathecal MVIIA injection after spinal cord injury (SCI) elicits neuroprotective effects. The test rats were randomly distributed into six groups— sham, placebo, MVIIA 2.5 μM, MVIIA 5 μM, MVIIA 10 μM, and MVIIA 20 μM—and were administered the treatment four hours after SCI. After the optimal MVIIA dose (MVIIA 10 μM) was defined, the best time for application, one or four hours, was analyzed. Locomotor hind limb function and side effects were assessed. Forty-eight hours after the injury and immediately after euthanasia, spinal cord segments were removed from the test rats. Cell viability, reactive oxygen species, lipid peroxidation, and glutamate release were investigated. To examine the MVIIA mechanism of action, the gene expressions of pro-apoptotic (Bax, nNOS, and caspase-3, -8, -9, -12) and anti-apoptotic (Bcl-xl) factors in the spinal cord tissue samples were determined by real-time PCR, and the activities of antioxidant enzymes were also investigated. Application of intrathecal MVIIA 10 μM four hours after SCI prompted a neuroprotective effect: neuronal death decreased (22.46%), oxidative stress diminished, pro-apoptotic factors (Bax, nNOS, and caspase-3, -8) were expressed to a lesser extent, and mitochondrial viability as well as anti-apoptotic factor (Bcl-xl) expression increased. These results suggested that MVIIA provided neuroprotection through antioxidant effects. Indeed, superoxide dismutase (188.41%), and glutathione peroxidase (199.96%), reductase (193.86%), and transferase (175.93%) expressions increased. Therefore, intrathecal MVIIA (MVIIA 10 μM, 4 h) application has neuroprotective potential, and the possible mechanisms are related to antioxidant agent modulation and to intrinsic and extrinsic apoptotic pathways.2019-04-22T11:17:07Z2019-04-22T11:17:07Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA, K. M. de et al. Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. PLoS One, v. 13, n. 10, p. 1-26, out. 2018. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204948>. Acesso em: 25 fev. 2019.1935-2735http://www.repositorio.ufop.br/handle/123456789/11071This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: o próprio artigo.info:eu-repo/semantics/openAccessOliveira, Karen Maciel deBinda, Nancy ScarduaLavor, Mário Sérgio Lima deSilva, Carla Maria OsórioRosado, Isabel RodriguesAlves, Endrigo Gabellini LeonelSilva, Juliana Figueira daOliveira, Camila M.Melo, Marilia MartinsGomez, Marcus ViniciusMelo, Eliane Gonçalves deengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-04-22T11:17:07Zoai:repositorio.ufop.br:123456789/11071Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-04-22T11:17:07Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. |
title |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. |
spellingShingle |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. Oliveira, Karen Maciel de |
title_short |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. |
title_full |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. |
title_fullStr |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. |
title_full_unstemmed |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. |
title_sort |
Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. |
author |
Oliveira, Karen Maciel de |
author_facet |
Oliveira, Karen Maciel de Binda, Nancy Scardua Lavor, Mário Sérgio Lima de Silva, Carla Maria Osório Rosado, Isabel Rodrigues Alves, Endrigo Gabellini Leonel Silva, Juliana Figueira da Oliveira, Camila M. Melo, Marilia Martins Gomez, Marcus Vinicius Melo, Eliane Gonçalves de |
author_role |
author |
author2 |
Binda, Nancy Scardua Lavor, Mário Sérgio Lima de Silva, Carla Maria Osório Rosado, Isabel Rodrigues Alves, Endrigo Gabellini Leonel Silva, Juliana Figueira da Oliveira, Camila M. Melo, Marilia Martins Gomez, Marcus Vinicius Melo, Eliane Gonçalves de |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Karen Maciel de Binda, Nancy Scardua Lavor, Mário Sérgio Lima de Silva, Carla Maria Osório Rosado, Isabel Rodrigues Alves, Endrigo Gabellini Leonel Silva, Juliana Figueira da Oliveira, Camila M. Melo, Marilia Martins Gomez, Marcus Vinicius Melo, Eliane Gonçalves de |
description |
This study evaluates whether intrathecal MVIIA injection after spinal cord injury (SCI) elicits neuroprotective effects. The test rats were randomly distributed into six groups— sham, placebo, MVIIA 2.5 μM, MVIIA 5 μM, MVIIA 10 μM, and MVIIA 20 μM—and were administered the treatment four hours after SCI. After the optimal MVIIA dose (MVIIA 10 μM) was defined, the best time for application, one or four hours, was analyzed. Locomotor hind limb function and side effects were assessed. Forty-eight hours after the injury and immediately after euthanasia, spinal cord segments were removed from the test rats. Cell viability, reactive oxygen species, lipid peroxidation, and glutamate release were investigated. To examine the MVIIA mechanism of action, the gene expressions of pro-apoptotic (Bax, nNOS, and caspase-3, -8, -9, -12) and anti-apoptotic (Bcl-xl) factors in the spinal cord tissue samples were determined by real-time PCR, and the activities of antioxidant enzymes were also investigated. Application of intrathecal MVIIA 10 μM four hours after SCI prompted a neuroprotective effect: neuronal death decreased (22.46%), oxidative stress diminished, pro-apoptotic factors (Bax, nNOS, and caspase-3, -8) were expressed to a lesser extent, and mitochondrial viability as well as anti-apoptotic factor (Bcl-xl) expression increased. These results suggested that MVIIA provided neuroprotection through antioxidant effects. Indeed, superoxide dismutase (188.41%), and glutathione peroxidase (199.96%), reductase (193.86%), and transferase (175.93%) expressions increased. Therefore, intrathecal MVIIA (MVIIA 10 μM, 4 h) application has neuroprotective potential, and the possible mechanisms are related to antioxidant agent modulation and to intrinsic and extrinsic apoptotic pathways. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2019-04-22T11:17:07Z 2019-04-22T11:17:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA, K. M. de et al. Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. PLoS One, v. 13, n. 10, p. 1-26, out. 2018. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204948>. Acesso em: 25 fev. 2019. 1935-2735 http://www.repositorio.ufop.br/handle/123456789/11071 |
identifier_str_mv |
OLIVEIRA, K. M. de et al. Conotoxin MVIIA improves cell viability and antioxidant system after spinal cord injury in rats. PLoS One, v. 13, n. 10, p. 1-26, out. 2018. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204948>. Acesso em: 25 fev. 2019. 1935-2735 |
url |
http://www.repositorio.ufop.br/handle/123456789/11071 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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1813002835551322112 |