Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/9410 https://doi.org/10.1038/srep33588 |
Resumo: | The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. |
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Munk, MicheleLadeira, Luiz OrlandoCarvalho, Bruno Campos deRaposo, Nádia Rezende BarbosaSerapião, Raquel V.Quintão, Carolina Capobiango RomanoSilva, Saulo RodriguesSoares, Jaqueline dos SantosJorio, AdoBrandão, Humberto de Mello2018-02-01T14:06:09Z2018-02-01T14:06:09Z2016MUNK, M. et al. Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. Scientific Reports, v. 6, p. 1-10, 2016. Disponível em: <https://www.nature.com/articles/srep33588>. Acesso em: 16 jan. 2018.2045-2322http://www.repositorio.ufop.br/handle/123456789/9410https://doi.org/10.1038/srep33588The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.Os trabalhos publicados no periódico Scientific Reports estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho desde que sejam citados o autor e o licenciante. Fonte: Sherpa/Romeo <http://www.sherpa.ac.uk/romeo/search.php?issn=2045-2322>. Acesso em: 27 fev. 2020.info:eu-repo/semantics/openAccessEfficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/9410/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_EfficientDeliveryDNA.pdfARTIGO_EfficientDeliveryDNA.pdfapplication/pdf1010167http://www.repositorio.ufop.br/bitstream/123456789/9410/1/ARTIGO_EfficientDeliveryDNA.pdfd585e35a6ea7d53b33a8aa99eb2e3fa9MD51123456789/94102020-02-27 09:28:22.695oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-02-27T14:28:22Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
title |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
spellingShingle |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. Munk, Michele |
title_short |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
title_full |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
title_fullStr |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
title_full_unstemmed |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
title_sort |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
author |
Munk, Michele |
author_facet |
Munk, Michele Ladeira, Luiz Orlando Carvalho, Bruno Campos de Raposo, Nádia Rezende Barbosa Serapião, Raquel V. Quintão, Carolina Capobiango Romano Silva, Saulo Rodrigues Soares, Jaqueline dos Santos Jorio, Ado Brandão, Humberto de Mello |
author_role |
author |
author2 |
Ladeira, Luiz Orlando Carvalho, Bruno Campos de Raposo, Nádia Rezende Barbosa Serapião, Raquel V. Quintão, Carolina Capobiango Romano Silva, Saulo Rodrigues Soares, Jaqueline dos Santos Jorio, Ado Brandão, Humberto de Mello |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Munk, Michele Ladeira, Luiz Orlando Carvalho, Bruno Campos de Raposo, Nádia Rezende Barbosa Serapião, Raquel V. Quintão, Carolina Capobiango Romano Silva, Saulo Rodrigues Soares, Jaqueline dos Santos Jorio, Ado Brandão, Humberto de Mello |
description |
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2018-02-01T14:06:09Z |
dc.date.available.fl_str_mv |
2018-02-01T14:06:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MUNK, M. et al. Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. Scientific Reports, v. 6, p. 1-10, 2016. Disponível em: <https://www.nature.com/articles/srep33588>. Acesso em: 16 jan. 2018. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/9410 |
dc.identifier.issn.none.fl_str_mv |
2045-2322 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1038/srep33588 |
identifier_str_mv |
MUNK, M. et al. Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. Scientific Reports, v. 6, p. 1-10, 2016. Disponível em: <https://www.nature.com/articles/srep33588>. Acesso em: 16 jan. 2018. 2045-2322 |
url |
http://www.repositorio.ufop.br/handle/123456789/9410 https://doi.org/10.1038/srep33588 |
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eng |
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eng |
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