Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/1010 |
Resumo: | The factors involved in the reactivation of chronic Chagas disease infection are not clear enough and may be related to host immune unbalance and/or parasite genetic diversity. To evaluate the role of the Trypanosoma cruzi genetic background in the Chagas disease reactivation, we inoculated Cyclophosphamide-immunos upressed (CyI) Swiss mice with clonal stocks from T. cruzi I (Cuica cl1, P209 cl1, Gamba cl1, SP104 cl1), T. cruzi II (IVV cl4, MVB cl8) and T. cruzi (Bug2148 cl1, MN cl2) lineages. We used the parasitemia as the parameter for Chagas disease reactivation and observed that CyI animals infected with T. cruzi stocks showed no reactivation and those infected with T. cruzi II stocks showed only 5% of reactivation. In contrast, immunosuppressed mice infected with stocks from T. cruzi I lineage showed 77.5 and 51.25% reactivation of the infection when Cyclophosphamide treatment was performed 60 and 180 days after inoculation, respectively. Next, we evaluated the efficacy of the Benznidazole (Bz) pre-treatment in reducing or preventing the recurrence of the infection in these CyI animals. In general, the percentage of the parasite recurrence was not altered among the CyI mice that received the Bz pretreatment during the acute phase of the infection. Interestingly, when pre-Bz treatment was performed during the chronic phase, we observed two different patterns of response: (i) an increased protection among the animals inoculated with the SP104 cl1 (genotype 19) and Cuica cl1 (genotype 20) stocks; (ii) an increased percentage of parasitemia reactivation among mice inoculated with Gamba cl1 (genotype 19) and P209 cl1 (genotype 20) T. cruzi stocks. Our results corroborate our hypothesis by showing that the T. cruzi genetic background in combination with specific Bz treatment has an important role in the Chagas disease reactivation in immunosuppressed animals. |
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Santos, Daniela Maria dosMartins, Tassiane Assíria FontesCaldas, Ivo SantanaDiniz, Lívia de FigueiredoCoelho, George Luiz Lins MachadoCarneiro, Cláudia MartinsOliveira, Riva de PaulaSilva, André Talvani Pedrosa daLana, Marta deBahia, Maria Terezinha2012-07-09T15:30:28Z2012-07-09T15:30:28Z2010SANTOS, D. M. dos et al. Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. Acta Tropica, v. 113, n. 2, p. 134-138, fev. 2010. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X09003271>. Acesso em: 09 jul. 2012.0001706Xhttp://www.repositorio.ufop.br/handle/123456789/1010The factors involved in the reactivation of chronic Chagas disease infection are not clear enough and may be related to host immune unbalance and/or parasite genetic diversity. To evaluate the role of the Trypanosoma cruzi genetic background in the Chagas disease reactivation, we inoculated Cyclophosphamide-immunos upressed (CyI) Swiss mice with clonal stocks from T. cruzi I (Cuica cl1, P209 cl1, Gamba cl1, SP104 cl1), T. cruzi II (IVV cl4, MVB cl8) and T. cruzi (Bug2148 cl1, MN cl2) lineages. We used the parasitemia as the parameter for Chagas disease reactivation and observed that CyI animals infected with T. cruzi stocks showed no reactivation and those infected with T. cruzi II stocks showed only 5% of reactivation. In contrast, immunosuppressed mice infected with stocks from T. cruzi I lineage showed 77.5 and 51.25% reactivation of the infection when Cyclophosphamide treatment was performed 60 and 180 days after inoculation, respectively. Next, we evaluated the efficacy of the Benznidazole (Bz) pre-treatment in reducing or preventing the recurrence of the infection in these CyI animals. In general, the percentage of the parasite recurrence was not altered among the CyI mice that received the Bz pretreatment during the acute phase of the infection. Interestingly, when pre-Bz treatment was performed during the chronic phase, we observed two different patterns of response: (i) an increased protection among the animals inoculated with the SP104 cl1 (genotype 19) and Cuica cl1 (genotype 20) stocks; (ii) an increased percentage of parasitemia reactivation among mice inoculated with Gamba cl1 (genotype 19) and P209 cl1 (genotype 20) T. cruzi stocks. Our results corroborate our hypothesis by showing that the T. cruzi genetic background in combination with specific Bz treatment has an important role in the Chagas disease reactivation in immunosuppressed animals.Cyclophosphamide immunosuppressionBenznidazoleTrypanosoma cruziGenetic diversityBenznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3280860188365.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPORIGINALARTIGO_BenznidazoleAltersPattern.pdfARTIGO_BenznidazoleAltersPattern.pdfapplication/pdf321263http://www.repositorio.ufop.br/bitstream/123456789/1010/1/ARTIGO_BenznidazoleAltersPattern.pdfaff4ad0c8c0e055425dbf67bfbae3ac4MD51123456789/10102019-02-25 12:40:45.865oai:localhost:123456789/1010Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-02-25T17:40:45Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. |
title |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. |
spellingShingle |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. Santos, Daniela Maria dos Cyclophosphamide immunosuppression Benznidazole Trypanosoma cruzi Genetic diversity |
title_short |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. |
title_full |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. |
title_fullStr |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. |
title_full_unstemmed |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. |
title_sort |
Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. |
author |
Santos, Daniela Maria dos |
author_facet |
Santos, Daniela Maria dos Martins, Tassiane Assíria Fontes Caldas, Ivo Santana Diniz, Lívia de Figueiredo Coelho, George Luiz Lins Machado Carneiro, Cláudia Martins Oliveira, Riva de Paula Silva, André Talvani Pedrosa da Lana, Marta de Bahia, Maria Terezinha |
author_role |
author |
author2 |
Martins, Tassiane Assíria Fontes Caldas, Ivo Santana Diniz, Lívia de Figueiredo Coelho, George Luiz Lins Machado Carneiro, Cláudia Martins Oliveira, Riva de Paula Silva, André Talvani Pedrosa da Lana, Marta de Bahia, Maria Terezinha |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Daniela Maria dos Martins, Tassiane Assíria Fontes Caldas, Ivo Santana Diniz, Lívia de Figueiredo Coelho, George Luiz Lins Machado Carneiro, Cláudia Martins Oliveira, Riva de Paula Silva, André Talvani Pedrosa da Lana, Marta de Bahia, Maria Terezinha |
dc.subject.por.fl_str_mv |
Cyclophosphamide immunosuppression Benznidazole Trypanosoma cruzi Genetic diversity |
topic |
Cyclophosphamide immunosuppression Benznidazole Trypanosoma cruzi Genetic diversity |
description |
The factors involved in the reactivation of chronic Chagas disease infection are not clear enough and may be related to host immune unbalance and/or parasite genetic diversity. To evaluate the role of the Trypanosoma cruzi genetic background in the Chagas disease reactivation, we inoculated Cyclophosphamide-immunos upressed (CyI) Swiss mice with clonal stocks from T. cruzi I (Cuica cl1, P209 cl1, Gamba cl1, SP104 cl1), T. cruzi II (IVV cl4, MVB cl8) and T. cruzi (Bug2148 cl1, MN cl2) lineages. We used the parasitemia as the parameter for Chagas disease reactivation and observed that CyI animals infected with T. cruzi stocks showed no reactivation and those infected with T. cruzi II stocks showed only 5% of reactivation. In contrast, immunosuppressed mice infected with stocks from T. cruzi I lineage showed 77.5 and 51.25% reactivation of the infection when Cyclophosphamide treatment was performed 60 and 180 days after inoculation, respectively. Next, we evaluated the efficacy of the Benznidazole (Bz) pre-treatment in reducing or preventing the recurrence of the infection in these CyI animals. In general, the percentage of the parasite recurrence was not altered among the CyI mice that received the Bz pretreatment during the acute phase of the infection. Interestingly, when pre-Bz treatment was performed during the chronic phase, we observed two different patterns of response: (i) an increased protection among the animals inoculated with the SP104 cl1 (genotype 19) and Cuica cl1 (genotype 20) stocks; (ii) an increased percentage of parasitemia reactivation among mice inoculated with Gamba cl1 (genotype 19) and P209 cl1 (genotype 20) T. cruzi stocks. Our results corroborate our hypothesis by showing that the T. cruzi genetic background in combination with specific Bz treatment has an important role in the Chagas disease reactivation in immunosuppressed animals. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2012-07-09T15:30:28Z |
dc.date.available.fl_str_mv |
2012-07-09T15:30:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, D. M. dos et al. Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. Acta Tropica, v. 113, n. 2, p. 134-138, fev. 2010. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X09003271>. Acesso em: 09 jul. 2012. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/1010 |
dc.identifier.issn.none.fl_str_mv |
0001706X |
identifier_str_mv |
SANTOS, D. M. dos et al. Benznidazole alters the pattern of Cyclophosphamide-induced reactivation in experimental Trypanosoma cruzi-dependent lineage infection. Acta Tropica, v. 113, n. 2, p. 134-138, fev. 2010. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X09003271>. Acesso em: 09 jul. 2012. 0001706X |
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http://www.repositorio.ufop.br/handle/123456789/1010 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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