Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/1403 |
Resumo: | Iron chelators have been employed in various studies aimed at evaluating the relationship between the iron status of the host and the development of infection. In the present study, the effects of benznidazole (BZ) therapy in combination with the iron chelator desferrioxamine (DFO) on the development of infection in mice inoculated with Trypanosoma cruzi Y strain have been investigated. Infected mice treated with DFO presented lower levels of parasitemia compared with infected untreated animals. Therapy with BZ for 21 days, with or without DFO, led to decreased parasitemia and reduced mortality, but BZ in combination with DFO treatment for 35 days (BZ/DFO-35) gave 0% mortality. All infected groups presented lower levels of iron in the liver, but serum iron concentrations were greater in DFO-35 and BZ/DFO-35, whereas hemoglobin levels were higher in BZ/DFO-35 and lower in DFO-35 compared with other treated groups. The percentage cure, determined from negative hemoculture and PCR results in animals that had survived for 60 days post-infection, was 18% for BZ and BZ/DFO-35, 42% for BZ combined with DFO for 21 days, and 67% for DFO-35. The results demonstrate that modification in iron stores increases BZ efficacy. |
id |
UFOP_b7d0652617d776d4b0b911cdfb233d2d |
---|---|
oai_identifier_str |
oai:localhost:123456789/1403 |
network_acronym_str |
UFOP |
network_name_str |
Repositório Institucional da UFOP |
repository_id_str |
3233 |
spelling |
Francisco, Amanda FortesVieira, Paula Melo de AbreuArantes, Jerusa MarildaPedrosa, Maria LúciaMartins, Helen RodriguesSilva, MaísaVeloso, Vanja MariaLana, Marta deBahia, Maria TerezinhaTafuri, Washington LuizCarneiro, Cláudia Martins2012-09-19T15:32:02Z2012-09-19T15:32:02Z2008FRANCISCO, A. M. et al. Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. Experimental Parasitology, v. 120, n. 4, p. 314-319, dez. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0014489408001975>. Acesso em: 19 ago. 2012.00144894http://www.repositorio.ufop.br/handle/123456789/1403Iron chelators have been employed in various studies aimed at evaluating the relationship between the iron status of the host and the development of infection. In the present study, the effects of benznidazole (BZ) therapy in combination with the iron chelator desferrioxamine (DFO) on the development of infection in mice inoculated with Trypanosoma cruzi Y strain have been investigated. Infected mice treated with DFO presented lower levels of parasitemia compared with infected untreated animals. Therapy with BZ for 21 days, with or without DFO, led to decreased parasitemia and reduced mortality, but BZ in combination with DFO treatment for 35 days (BZ/DFO-35) gave 0% mortality. All infected groups presented lower levels of iron in the liver, but serum iron concentrations were greater in DFO-35 and BZ/DFO-35, whereas hemoglobin levels were higher in BZ/DFO-35 and lower in DFO-35 compared with other treated groups. The percentage cure, determined from negative hemoculture and PCR results in animals that had survived for 60 days post-infection, was 18% for BZ and BZ/DFO-35, 42% for BZ combined with DFO for 21 days, and 67% for DFO-35. The results demonstrate that modification in iron stores increases BZ efficacy.Trypanosoma cruziChagas' diseaseReactive oxygen speciesAnalysis of varianceDeoxyribonucleic acidTrypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Experimental Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3281990828453.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/1403/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALARTIGO_TrypanosomaCruziEffect.pdfARTIGO_TrypanosomaCruziEffect.pdfapplication/pdf266779http://www.repositorio.ufop.br/bitstream/123456789/1403/1/ARTIGO_TrypanosomaCruziEffect.pdf9bdc190a3a98a61ded3ea8022cc93bbcMD51123456789/14032019-03-08 11:30:35.766oai:localhost:123456789/1403Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-03-08T16:30:35Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. |
title |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. |
spellingShingle |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. Francisco, Amanda Fortes Trypanosoma cruzi Chagas' disease Reactive oxygen species Analysis of variance Deoxyribonucleic acid |
title_short |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. |
title_full |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. |
title_fullStr |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. |
title_full_unstemmed |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. |
title_sort |
Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. |
author |
Francisco, Amanda Fortes |
author_facet |
Francisco, Amanda Fortes Vieira, Paula Melo de Abreu Arantes, Jerusa Marilda Pedrosa, Maria Lúcia Martins, Helen Rodrigues Silva, Maísa Veloso, Vanja Maria Lana, Marta de Bahia, Maria Terezinha Tafuri, Washington Luiz Carneiro, Cláudia Martins |
author_role |
author |
author2 |
Vieira, Paula Melo de Abreu Arantes, Jerusa Marilda Pedrosa, Maria Lúcia Martins, Helen Rodrigues Silva, Maísa Veloso, Vanja Maria Lana, Marta de Bahia, Maria Terezinha Tafuri, Washington Luiz Carneiro, Cláudia Martins |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Francisco, Amanda Fortes Vieira, Paula Melo de Abreu Arantes, Jerusa Marilda Pedrosa, Maria Lúcia Martins, Helen Rodrigues Silva, Maísa Veloso, Vanja Maria Lana, Marta de Bahia, Maria Terezinha Tafuri, Washington Luiz Carneiro, Cláudia Martins |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Chagas' disease Reactive oxygen species Analysis of variance Deoxyribonucleic acid |
topic |
Trypanosoma cruzi Chagas' disease Reactive oxygen species Analysis of variance Deoxyribonucleic acid |
description |
Iron chelators have been employed in various studies aimed at evaluating the relationship between the iron status of the host and the development of infection. In the present study, the effects of benznidazole (BZ) therapy in combination with the iron chelator desferrioxamine (DFO) on the development of infection in mice inoculated with Trypanosoma cruzi Y strain have been investigated. Infected mice treated with DFO presented lower levels of parasitemia compared with infected untreated animals. Therapy with BZ for 21 days, with or without DFO, led to decreased parasitemia and reduced mortality, but BZ in combination with DFO treatment for 35 days (BZ/DFO-35) gave 0% mortality. All infected groups presented lower levels of iron in the liver, but serum iron concentrations were greater in DFO-35 and BZ/DFO-35, whereas hemoglobin levels were higher in BZ/DFO-35 and lower in DFO-35 compared with other treated groups. The percentage cure, determined from negative hemoculture and PCR results in animals that had survived for 60 days post-infection, was 18% for BZ and BZ/DFO-35, 42% for BZ combined with DFO for 21 days, and 67% for DFO-35. The results demonstrate that modification in iron stores increases BZ efficacy. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2012-09-19T15:32:02Z |
dc.date.available.fl_str_mv |
2012-09-19T15:32:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FRANCISCO, A. M. et al. Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. Experimental Parasitology, v. 120, n. 4, p. 314-319, dez. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0014489408001975>. Acesso em: 19 ago. 2012. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/1403 |
dc.identifier.issn.none.fl_str_mv |
00144894 |
identifier_str_mv |
FRANCISCO, A. M. et al. Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice. Experimental Parasitology, v. 120, n. 4, p. 314-319, dez. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0014489408001975>. Acesso em: 19 ago. 2012. 00144894 |
url |
http://www.repositorio.ufop.br/handle/123456789/1403 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
bitstream.url.fl_str_mv |
http://www.repositorio.ufop.br/bitstream/123456789/1403/5/license.txt http://www.repositorio.ufop.br/bitstream/123456789/1403/1/ARTIGO_TrypanosomaCruziEffect.pdf |
bitstream.checksum.fl_str_mv |
8a4605be74aa9ea9d79846c1fba20a33 9bdc190a3a98a61ded3ea8022cc93bbc |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
_version_ |
1801685738595549184 |