Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/9931 https://www.sciencedirect.com/science/article/pii/S1385894717319472 |
Resumo: | This study was designed to obtain simultaneous sulfate (SO4 2−) and As(III) ions removal by non-traditional sulfate-reducing bacteria (SRB) growing in the presence of a protein biomass (PCF), a solid residual material produced by the poultry industry. Microbial cells and PCF were immobilized into calcium alginate beads to reduce mass losses. Microbial consortium efficiency was tested under different operational conditions: sodium lactate, glycerol and PCF as carbon sources; increasing As(III) content; acidic pH (5.0). Microbial diversity was evaluated by PCR-DGGE. Pantoea agglomerans, Enterobacter sp., Citrobacter sp., Cupriavidus metallidurans, Ralstonia sp. and Burkholderia cepacia were found. With the aim to obtain SO4 2− and As(III) simultaneous removal, three semi-continuous up-flow reactors were constructed and operated for more than 100 days to prove their feasibility and reliability. Both SO4 2− and As(III) ions were removed with 74.8% and 80% efficiency, respectively. Solid products were characterized by SEM-EDX, confirming PCF and non-traditional SRB roles during arsenic immobilization. Arsenic/sulfur compounds (possibly As/sulfides) were produced by reaction between As(III) and biogenic sulfide. Microbial consortium proved its ability of growing in an acidic environment without losing its sulfate reductive capacity by using glycerol and PCF as alternative carbon sources and tap water as micronutrients source. The non-traditional SRB culture removed both SO4 2− and As(III) ions simultaneously, with no prior step needed to guarantee As(III) oxidation to As(V). Two different mechanisms could be involved: (i) precipitation as arsenic sulfide or, (ii) adsorption onto PCF/cells calcium alginate beads. |
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Matos, Letícia Paiva deCosta, Patrícia FreitasMore, MarianaGomes, Paula Cristine SilvaSilva, Silvana de QueirozGurgel, Leandro Vinícius AlvesTeixeira, Mônica Cristina2018-05-14T14:45:06Z2018-05-14T14:45:06Z2017MATOS, L. P. de et al. Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. Chemical Engineering Journal, v. 334, p. 1630-1641, 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1385894717319472>. Acesso em: 05 abr. 2018. 13858947http://www.repositorio.ufop.br/handle/123456789/9931https://www.sciencedirect.com/science/article/pii/S1385894717319472This study was designed to obtain simultaneous sulfate (SO4 2−) and As(III) ions removal by non-traditional sulfate-reducing bacteria (SRB) growing in the presence of a protein biomass (PCF), a solid residual material produced by the poultry industry. Microbial cells and PCF were immobilized into calcium alginate beads to reduce mass losses. Microbial consortium efficiency was tested under different operational conditions: sodium lactate, glycerol and PCF as carbon sources; increasing As(III) content; acidic pH (5.0). Microbial diversity was evaluated by PCR-DGGE. Pantoea agglomerans, Enterobacter sp., Citrobacter sp., Cupriavidus metallidurans, Ralstonia sp. and Burkholderia cepacia were found. With the aim to obtain SO4 2− and As(III) simultaneous removal, three semi-continuous up-flow reactors were constructed and operated for more than 100 days to prove their feasibility and reliability. Both SO4 2− and As(III) ions were removed with 74.8% and 80% efficiency, respectively. Solid products were characterized by SEM-EDX, confirming PCF and non-traditional SRB roles during arsenic immobilization. Arsenic/sulfur compounds (possibly As/sulfides) were produced by reaction between As(III) and biogenic sulfide. Microbial consortium proved its ability of growing in an acidic environment without losing its sulfate reductive capacity by using glycerol and PCF as alternative carbon sources and tap water as micronutrients source. The non-traditional SRB culture removed both SO4 2− and As(III) ions simultaneously, with no prior step needed to guarantee As(III) oxidation to As(V). Two different mechanisms could be involved: (i) precipitation as arsenic sulfide or, (ii) adsorption onto PCF/cells calcium alginate beads.GlycerolSemi-continuous up-flow bioreactorArseniteCalcium alginate beadsSimultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/9931/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_SimultaneousRemovalSulfate.pdfARTIGO_SimultaneousRemovalSulfate.pdfapplication/pdf1532353http://www.repositorio.ufop.br/bitstream/123456789/9931/1/ARTIGO_SimultaneousRemovalSulfate.pdf5a3d8844533714f1ba266605cdf98af4MD51123456789/99312018-05-14 10:45:30.898oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332018-05-14T14:45:30Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. |
title |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. |
spellingShingle |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. Matos, Letícia Paiva de Glycerol Semi-continuous up-flow bioreactor Arsenite Calcium alginate beads |
title_short |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. |
title_full |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. |
title_fullStr |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. |
title_full_unstemmed |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. |
title_sort |
Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. |
author |
Matos, Letícia Paiva de |
author_facet |
Matos, Letícia Paiva de Costa, Patrícia Freitas More, Mariana Gomes, Paula Cristine Silva Silva, Silvana de Queiroz Gurgel, Leandro Vinícius Alves Teixeira, Mônica Cristina |
author_role |
author |
author2 |
Costa, Patrícia Freitas More, Mariana Gomes, Paula Cristine Silva Silva, Silvana de Queiroz Gurgel, Leandro Vinícius Alves Teixeira, Mônica Cristina |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Matos, Letícia Paiva de Costa, Patrícia Freitas More, Mariana Gomes, Paula Cristine Silva Silva, Silvana de Queiroz Gurgel, Leandro Vinícius Alves Teixeira, Mônica Cristina |
dc.subject.por.fl_str_mv |
Glycerol Semi-continuous up-flow bioreactor Arsenite Calcium alginate beads |
topic |
Glycerol Semi-continuous up-flow bioreactor Arsenite Calcium alginate beads |
description |
This study was designed to obtain simultaneous sulfate (SO4 2−) and As(III) ions removal by non-traditional sulfate-reducing bacteria (SRB) growing in the presence of a protein biomass (PCF), a solid residual material produced by the poultry industry. Microbial cells and PCF were immobilized into calcium alginate beads to reduce mass losses. Microbial consortium efficiency was tested under different operational conditions: sodium lactate, glycerol and PCF as carbon sources; increasing As(III) content; acidic pH (5.0). Microbial diversity was evaluated by PCR-DGGE. Pantoea agglomerans, Enterobacter sp., Citrobacter sp., Cupriavidus metallidurans, Ralstonia sp. and Burkholderia cepacia were found. With the aim to obtain SO4 2− and As(III) simultaneous removal, three semi-continuous up-flow reactors were constructed and operated for more than 100 days to prove their feasibility and reliability. Both SO4 2− and As(III) ions were removed with 74.8% and 80% efficiency, respectively. Solid products were characterized by SEM-EDX, confirming PCF and non-traditional SRB roles during arsenic immobilization. Arsenic/sulfur compounds (possibly As/sulfides) were produced by reaction between As(III) and biogenic sulfide. Microbial consortium proved its ability of growing in an acidic environment without losing its sulfate reductive capacity by using glycerol and PCF as alternative carbon sources and tap water as micronutrients source. The non-traditional SRB culture removed both SO4 2− and As(III) ions simultaneously, with no prior step needed to guarantee As(III) oxidation to As(V). Two different mechanisms could be involved: (i) precipitation as arsenic sulfide or, (ii) adsorption onto PCF/cells calcium alginate beads. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-05-14T14:45:06Z |
dc.date.available.fl_str_mv |
2018-05-14T14:45:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MATOS, L. P. de et al. Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. Chemical Engineering Journal, v. 334, p. 1630-1641, 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1385894717319472>. Acesso em: 05 abr. 2018. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/9931 |
dc.identifier.issn.none.fl_str_mv |
13858947 |
dc.identifier.uri2.pt_BR.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S1385894717319472 |
identifier_str_mv |
MATOS, L. P. de et al. Simultaneous removal of sulfate and arsenic using immobilized nontraditional SRB mixed culture and alternative low-cost carbon sources. Chemical Engineering Journal, v. 334, p. 1630-1641, 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1385894717319472>. Acesso em: 05 abr. 2018. 13858947 |
url |
http://www.repositorio.ufop.br/handle/123456789/9931 https://www.sciencedirect.com/science/article/pii/S1385894717319472 |
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eng |
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