Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/4626 https://doi.org/10.1186/1743-7075-9-63 |
Resumo: | Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P<0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation. |
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Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.Fructose fed ratsHigh salt dietParaoxonaseAtherosclerosisParaoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P<0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation.2015-03-13T13:15:37Z2015-03-13T13:15:37Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfDORNAS, W. C. et al. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. Nutrition & Metabolism, v. 9, p. 63, 2012. Disponível em: <http://www.nutritionandmetabolism.com/content/9/1/63>. Acesso em: 08 nov. 2014.1743-7075http://www.repositorio.ufop.br/handle/123456789/4626https://doi.org/10.1186/1743-7075-9-63This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: o próprio artigo.info:eu-repo/semantics/openAccessDornas, Waleska Claudia AmaralLima, Wanderson Geraldo deSantos, Rinaldo Cardoso dosSouza, Melina Oliveira deSilva, MaísaDiniz, Mirla FiuzaSilva, Marcelo Eustáquioengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-24T19:07:13Zoai:repositorio.ufop.br:123456789/4626Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-24T19:07:13Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. |
title |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. |
spellingShingle |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. Dornas, Waleska Claudia Amaral Fructose fed rats High salt diet Paraoxonase Atherosclerosis |
title_short |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. |
title_full |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. |
title_fullStr |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. |
title_full_unstemmed |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. |
title_sort |
Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. |
author |
Dornas, Waleska Claudia Amaral |
author_facet |
Dornas, Waleska Claudia Amaral Lima, Wanderson Geraldo de Santos, Rinaldo Cardoso dos Souza, Melina Oliveira de Silva, Maísa Diniz, Mirla Fiuza Silva, Marcelo Eustáquio |
author_role |
author |
author2 |
Lima, Wanderson Geraldo de Santos, Rinaldo Cardoso dos Souza, Melina Oliveira de Silva, Maísa Diniz, Mirla Fiuza Silva, Marcelo Eustáquio |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Dornas, Waleska Claudia Amaral Lima, Wanderson Geraldo de Santos, Rinaldo Cardoso dos Souza, Melina Oliveira de Silva, Maísa Diniz, Mirla Fiuza Silva, Marcelo Eustáquio |
dc.subject.por.fl_str_mv |
Fructose fed rats High salt diet Paraoxonase Atherosclerosis |
topic |
Fructose fed rats High salt diet Paraoxonase Atherosclerosis |
description |
Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P<0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2015-03-13T13:15:37Z 2015-03-13T13:15:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
DORNAS, W. C. et al. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. Nutrition & Metabolism, v. 9, p. 63, 2012. Disponível em: <http://www.nutritionandmetabolism.com/content/9/1/63>. Acesso em: 08 nov. 2014. 1743-7075 http://www.repositorio.ufop.br/handle/123456789/4626 https://doi.org/10.1186/1743-7075-9-63 |
identifier_str_mv |
DORNAS, W. C. et al. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. Nutrition & Metabolism, v. 9, p. 63, 2012. Disponível em: <http://www.nutritionandmetabolism.com/content/9/1/63>. Acesso em: 08 nov. 2014. 1743-7075 |
url |
http://www.repositorio.ufop.br/handle/123456789/4626 https://doi.org/10.1186/1743-7075-9-63 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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1813002816234455040 |