Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.

Detalhes bibliográficos
Autor(a) principal: Dornas, Waleska Claudia Amaral
Data de Publicação: 2012
Outros Autores: Lima, Wanderson Geraldo de, Santos, Rinaldo Cardoso dos, Souza, Melina Oliveira de, Silva, Maísa, Diniz, Mirla Fiuza, Silva, Marcelo Eustáquio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4626
https://doi.org/10.1186/1743-7075-9-63
Resumo: Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P<0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation.
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spelling Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.Fructose fed ratsHigh salt dietParaoxonaseAtherosclerosisParaoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P<0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation.2015-03-13T13:15:37Z2015-03-13T13:15:37Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfDORNAS, W. C. et al. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. Nutrition & Metabolism, v. 9, p. 63, 2012. Disponível em: <http://www.nutritionandmetabolism.com/content/9/1/63>. Acesso em: 08 nov. 2014.1743-7075http://www.repositorio.ufop.br/handle/123456789/4626https://doi.org/10.1186/1743-7075-9-63This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: o próprio artigo.info:eu-repo/semantics/openAccessDornas, Waleska Claudia AmaralLima, Wanderson Geraldo deSantos, Rinaldo Cardoso dosSouza, Melina Oliveira deSilva, MaísaDiniz, Mirla FiuzaSilva, Marcelo Eustáquioengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-24T19:07:13Zoai:repositorio.ufop.br:123456789/4626Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-24T19:07:13Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
title Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
spellingShingle Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
Dornas, Waleska Claudia Amaral
Fructose fed rats
High salt diet
Paraoxonase
Atherosclerosis
title_short Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
title_full Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
title_fullStr Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
title_full_unstemmed Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
title_sort Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
author Dornas, Waleska Claudia Amaral
author_facet Dornas, Waleska Claudia Amaral
Lima, Wanderson Geraldo de
Santos, Rinaldo Cardoso dos
Souza, Melina Oliveira de
Silva, Maísa
Diniz, Mirla Fiuza
Silva, Marcelo Eustáquio
author_role author
author2 Lima, Wanderson Geraldo de
Santos, Rinaldo Cardoso dos
Souza, Melina Oliveira de
Silva, Maísa
Diniz, Mirla Fiuza
Silva, Marcelo Eustáquio
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dornas, Waleska Claudia Amaral
Lima, Wanderson Geraldo de
Santos, Rinaldo Cardoso dos
Souza, Melina Oliveira de
Silva, Maísa
Diniz, Mirla Fiuza
Silva, Marcelo Eustáquio
dc.subject.por.fl_str_mv Fructose fed rats
High salt diet
Paraoxonase
Atherosclerosis
topic Fructose fed rats
High salt diet
Paraoxonase
Atherosclerosis
description Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P<0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation.
publishDate 2012
dc.date.none.fl_str_mv 2012
2015-03-13T13:15:37Z
2015-03-13T13:15:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv DORNAS, W. C. et al. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. Nutrition & Metabolism, v. 9, p. 63, 2012. Disponível em: <http://www.nutritionandmetabolism.com/content/9/1/63>. Acesso em: 08 nov. 2014.
1743-7075
http://www.repositorio.ufop.br/handle/123456789/4626
https://doi.org/10.1186/1743-7075-9-63
identifier_str_mv DORNAS, W. C. et al. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity. Nutrition & Metabolism, v. 9, p. 63, 2012. Disponível em: <http://www.nutritionandmetabolism.com/content/9/1/63>. Acesso em: 08 nov. 2014.
1743-7075
url http://www.repositorio.ufop.br/handle/123456789/4626
https://doi.org/10.1186/1743-7075-9-63
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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